Mouse Anti-HBEGF Recombinant Antibody (clone 8d71f62f7) (CAT#: HPAB-0095-LS)

Figure 1 The results of an invasion assay on MDA-231 breast carcinoma cell line.

The invasiveness of MDA-231 cells through matrigel in the presence of 500 nM cross-specific monoclonal antibody (8d7 1c8 1e9 i.e. "1E9", 8d7 1f6 2f7 i.e. "2F7" or 8d7 1f6 2b3 i.e. "2B3" and negative control (iso-1) antibodies was assessed. The cells were left for 24 hours before being stained by Hoescht and number of invaded cells per field of view counted. Panel a is a barchart summarising the results with each antibody with Panel b showing representative images obtained using negative control antibodies and 2B3 antibodies ** indicates P<0.001. * indicates p<0.05.

Figure 2 The results of a migration assay on MDA-231 breast carcinoma cell line.

Assessed in the presence of 300 nM cross-specific monoclonal antibodies (8d7 1f6 2f7 i.e. "2F7" or 8d7 1c8 1e9 i.e. "1E9") or isotype control. The assay was left for 17 hrs before wound width was measured. The right hand panels showing representative images obtained in the absence of treatment and in the presence of 8d7 1f6 2b3 i.e. "2B3"

Figure 3 The results of an invasion assay of HUVEC cells through matrigel in the presence of 500 nM Bi-specific monoclonal antibody ((8d7 1f6 2f7 i.e. “2F7” or 8d7 1c8 1e9 i.e. “1E9” and negative control antibodies.

The cells were left for 24 hours before being stained by Hoescht and number of invaded cells per field of view counted.

Figure 4 Tube assay analysis of cross-specific antibody.

Huvec cells were seeded in the presence of cross-specific antibodies or control antibodies in wells coated with matrigel. Tube structure was analysed after 17 hrs. Images were taken and branching points around nodes counted. The lower panels show representative images with the upper panel illustrating in barchart format the number of branching points around nodes for untreated cells, cells treated with a control antibody and cells treated with the 8d7 1f6 2f7 antibodies.

Figure 5 The results of a migration assay of HUVEC cells in the presence of 500 nM cross-specific monoclonal antibody and negative control antibodies using a Boyden chamber assay.

The cells were left for 24 hours before being stained by Hoescht and number of invaded cells per field of view counted. * p<0.05, ** p<0.001 8d7 1c8 1e9 antibody, 8d7 1f6 2f7 antibody and 8d7 1f6 2b3