Recombinant Mouse Anti-HBV preS2 Antibody (F124) (CAT#: PABZ-054)

Recombinant Mouse Antibody (F124) is capable of binding to HBV preS2, expressed in Chinese Hamster Ovary cells (CHO). F124 recognizes an epitope contained within the preS2 segment between residues 120 and 132 of the surface antigen of hepatitis B virus.


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  • Published Data
  • Tested Data
  • Datasheet
  • MSDS
  • COA

Figure 1 PreS2-mediated translocation across the plasma membrane into the cytoplasm is an energy-independent process.

Figure 1 PreS2-mediated translocation across the plasma membrane into the cytoplasm is an energy-independent process.

Western blot analysis of the cytosolic fraction of HepG2 and 293 cells after 10 min and 30 min incubation with PreS1-PreS2 at concentrations of 2 mm at 37°C and 4°C. Western blot analysis was carried out using a primary antibody mixture (Q19–10, F124, HBV 116). The upper band in the left panel correlates with the PreS1-PreS2 dimer.

Oess, S., & Hildt, E. (2000). Novel cell permeable motif derived from the PreS2-domain of hepatitis-B virus surface antigens. Gene therapy, 7(9), 750.

Figure 2 PreS2 cell permeability is not restricted to hepatocytes.

Figure 2 PreS2 cell permeability is not restricted to hepatocytes.

(a–i) Immunofluorescence of HeLa cells (top panels a–c) after 30 min incubation and 293 cells (middle panels d–f) after 120 min incubation (a) without exogenous peptide; (d) with unrelated peptide (2.5 μm); (b,e) PreS2 (2.5 μm); (c,f) PreS2–3S-mutant (2.5 μm); (g–i) Immunofluorescence of HepG2 cells after 60 min incubation (g) without exogenous peptide at 37°C; (h) with 2 μm PreS1-PreS2 at 37°C and (i) with 2 μm PreS1-PreS2 at 4°C.

Oess, S., & Hildt, E. (2000). Novel cell permeable motif derived from the PreS2-domain of hepatitis-B virus surface antigens. Gene therapy, 7(9), 750.


Specifications

  • Immunogen
  • HBV PreS1
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native HBV preS2
  • Species Reactivity
  • HBV
  • Clone
  • F124
  • Applications
  • WB 1:1000-1:5000
    IF 1:100-1:500
    FuncS Assay-Dependent

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody F124 has been reported in applications of WB, IF, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • long surface protein; PreS1; L protein; LHBs; large S protein; pre-S1/pre-S2/S regions; L glycoprotein; L-HBsAG; LHBs

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone F124"

See other products for "HBV PreS2"

Recombinant Antibody

CAT Product Name Application Type
MOB-438 Recombinant Anti-HBV PreS2 Antibody ELISA, WB, FuncS IgG
MHH-438 Recombinant Human Anti-HBV PreS2 Antibody ELISA, IP, FuncS IgG
MRO-647CQ Recombinant Mouse Anti-HBV preS2 Antibody (S9) IHC-P, IHC-Fr, WB, ELISA, IF Mouse antibody
MRO-648CQ Recombinant Mouse Anti-HBV preS2 Antibody (M0071926) ELISA Mouse antibody
FAMAB-0246JF Mouse Anti-HBV preS2 Recombinant Antibody (clone 1F6) WB, ELISA, IHC, FC Mouse IgG

Chimeric Antibody

CAT Product Name Application Type
MRO-463LC Anti-HBV Pre-S2 Recombinant Antibody (A-44) ELISA Chimeric antibody (mouse/human)
MRO-463LC-S(P) Anti-HBV Pre-S2 Recombinant Antibody scFv Fragment (A-44) ELISA Chimeric antibody (mouse/human)
MRO-464LC-S(P) Anti-HBV Pre-S2 Recombinant Antibody scFv Fragment (6-31) ELISA Chimeric antibody (mouse/human)
MRO-463LC-F(E) Anti-HBV Pre-S2 Recombinant Antibody Fab Fragment (A-44) ELISA Chimeric antibody (mouse/human)
MRO-464LC-F(E) Anti-HBV Pre-S2 Recombinant Antibody Fab Fragment (6-31) ELISA Chimeric antibody (mouse/human)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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