Recombinant Mouse Anti-HCV E2 Antibody (AP33) (CAT#: PABL-496)

Recombinant Mouse Antibody (AP33) is capable of binding to HCV E2 , expressed in Chinese Hamster Ovary cells (CHO). This mouse monoclonal antibody recognizes a conserved, linear epitope on E2 and potently neutralizes a broad range of HCV genotypes.


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FC

Figure 1 Inhibition of HCV-LPs binding to cells by anti-E1 and anti-E2 antibodies.

Figure 1 Inhibition of HCV-LPs binding to cells by anti-E1 and anti-E2 antibodies.

SYTO-labeled HCV-LPs were pre-incubated with 20 to 100μg of anti-E2 (AP33) for 2h at 4°C. The HCV-LP-antibody mixtures were then incubated with Molt-4 cells for 1 h. Flow cytometry histogram of HCV-LPs binding inthe presence (20 μg/ml) (open graph) or absence (black graph) of antibodies. The background binding is shown as the gray graph.

Triyatni, M., Saunier, B., Maruvada, P., Davis, A. R., Ulianich, L., Heller, T., ... & Liang, T. J. (2002). Interaction of hepatitis C virus-like particles and cells: a model system for studying viral binding and entry. Journal of virology, 76(18), 9335-9344.

Neut

Figure 2 Neutralization of HCVpp of genotype 1a using AP33 and e509 combinations.

Figure 2 Neutralization of HCVpp of genotype 1a using AP33 and e509 combinations.

Increasing concentrations of AP33 alone and in combination with e509 mAb at a fixed saturating concentration (10 μg/mL). The concentration of each mAb, alone or in combination, is reported on x axis. The neutralizing activity (y axis) is expressed as percent reduction infectivity of HCVpp of genotype 1a (H77 strain) without mAbs.

Sautto, G., Mancini, N., Diotti, R. A., Solforosi, L., Clementi, M., & Burioni, R. (2012). Anti-hepatitis C virus E2 (HCV/E2) glycoprotein monoclonal antibodies and neutralization interference. Antiviral research, 96(1), 82-89.

FuncS

Figure 3 Identification and characterization of mutant Jc1 and Con1/C3-neo HCVcc resistant to E2412-423-specific neutralizing antibody (AP33).

Figure 3 Identification and characterization of mutant Jc1 and Con1/C3-neo HCVcc resistant to E2412-423-specific neutralizing antibody (AP33).

Supernatants and cells from Jc1 (squares) and Con1/C3-neo (circles) HCVcc-infected Huh7.5 cells were sequentially cultured in the absence (filled symbols) or presence (open symbols) of increasing concentrations of AP33, and HCV RNA replication was measured at various times post infection.

Pantua, H., Diao, J., Ultsch, M., Hazen, M., Mathieu, M., McCutcheon, K., ... & Hass, P. (2013). Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies. Journal of molecular biology, 425(11), 1899-1914.


Specifications

  • Immunogen
  • HCV E2 envelope glycoprotein
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native HCV E2
  • Species Reactivity
  • HCV
  • Clone
  • AP33
  • Applications
  • FC, Neut, FuncS

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • This antibody has been reported for use in Neutralization, Flow Cytometry and Function Assay.

Target

  • Alternative Names
  • E2; E2 (HCV); HCV E2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
MOB-446 Recombinant Anti-HCV E2 Antibody ELISA, WB, Dot, FuncS IgG
MRO-686CQ Recombinant Mouse Anti-HCV E2 Antibody (4F6/2) ELISA, IF, IP, WB Mouse antibody
MRO-687CQ Recombinant Mouse Anti-HCV E2 Antibody (M02061822) ELISA, WB, IF Mouse antibody
MRO-688CQ Recombinant Mouse Anti-HCV E2 Antibody (1876) ELISA, IF, WB Mouse antibody
MRO-689CQ Recombinant Mouse Anti-HCV E2 Antibody (6F6/I8) ELISA Mouse antibody

Mouse Antibody

CAT Product Name Application Type
MRO-1191LC Anti-HCV E2 Recombinant Antibody (A10F2) ELISA, Inhib Mouse Antibody
MRO-1192LC Anti-HCV E2 Recombinant Antibody (H6D3) ELISA, Inhib, WB Mouse Antibody
MRO-1193LC Anti-HCV E2 Recombinant Antibody (E3D8) ELISA, Inhib, WB Mouse Antibody
MRO-1194LC Anti-HCV E2 Recombinant Antibody (A7A6H9) ELISA, Inhib Mouse Antibody
MRO-1195LC Anti-HCV E2 Recombinant Antibody (G9E1) ELISA, Inhib Mouse Antibody

MHC Tetramer for Virology

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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