Recombinant Mouse Antibody (AP33) is capable of binding to HCV E2 , expressed in Chinese Hamster Ovary cells (CHO). This mouse monoclonal antibody recognizes a conserved, linear epitope on E2 and potently neutralizes a broad range of HCV genotypes.
Figure 1 Inhibition of HCV-LPs binding to cells by anti-E1 and anti-E2 antibodies.
SYTO-labeled HCV-LPs were pre-incubated with 20 to 100μg of anti-E2 (AP33) for 2h at 4°C. The HCV-LP-antibody mixtures were then incubated with Molt-4 cells for 1 h. Flow cytometry histogram of HCV-LPs binding inthe presence (20 μg/ml) (open graph) or absence (black graph) of antibodies. The background binding is shown as the gray graph.
Triyatni, M., Saunier, B., Maruvada, P., Davis, A. R., Ulianich, L., Heller, T., ... & Liang, T. J. (2002). Interaction of hepatitis C virus-like particles and cells: a model system for studying viral binding and entry. Journal of virology, 76(18), 9335-9344.
Figure 2 Neutralization of HCVpp of genotype 1a using AP33 and e509 combinations.
Increasing concentrations of AP33 alone and in combination with e509 mAb at a fixed saturating concentration (10 μg/mL). The concentration of each mAb, alone or in combination, is reported on x axis. The neutralizing activity (y axis) is expressed as percent reduction infectivity of HCVpp of genotype 1a (H77 strain) without mAbs.
Sautto, G., Mancini, N., Diotti, R. A., Solforosi, L., Clementi, M., & Burioni, R. (2012). Anti-hepatitis C virus E2 (HCV/E2) glycoprotein monoclonal antibodies and neutralization interference. Antiviral research, 96(1), 82-89.
Figure 3 Identification and characterization of mutant Jc1 and Con1/C3-neo HCVcc resistant to E2412-423-specific neutralizing antibody (AP33).
Supernatants and cells from Jc1 (squares) and Con1/C3-neo (circles) HCVcc-infected Huh7.5 cells were sequentially cultured in the absence (filled symbols) or presence (open symbols) of increasing concentrations of AP33, and HCV RNA replication was measured at various times post infection.
Pantua, H., Diao, J., Ultsch, M., Hazen, M., Mathieu, M., McCutcheon, K., ... & Hass, P. (2013). Glycan shifting on hepatitis C virus (HCV) E2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies. Journal of molecular biology, 425(11), 1899-1914.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-446 | Recombinant Anti-HCV E2 Antibody | ELISA, WB, Dot, FuncS | IgG |
MRO-686CQ | Recombinant Mouse Anti-HCV E2 Antibody (4F6/2) | ELISA, IF, IP, WB | Mouse antibody |
MRO-687CQ | Recombinant Mouse Anti-HCV E2 Antibody (M02061822) | ELISA, WB, IF | Mouse antibody |
MRO-688CQ | Recombinant Mouse Anti-HCV E2 Antibody (1876) | ELISA, IF, WB | Mouse antibody |
MRO-689CQ | Recombinant Mouse Anti-HCV E2 Antibody (6F6/I8) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-476LC | Human Anti-HCV E2 Protein Recombinant Antibody (MRO-476LC) | ELISA, Inhib | Chimeric (mouse/human) IgG |
MRO-476LC-S(P) | Mouse Anti-HCV E2 Protein Recombinant Antibody; scFv Fragment (MRO-476LC-S(P)) | ELISA | Mouse scFv |
MRO-476LC-F(E) | Human Anti-HCV E2 Protein Recombinant Antibody; Fab Fragment (MRO-476LC-F(E)) | ELISA | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-477LC | Human Anti-HCV E2 Protein Recombinant Antibody (MRO-477LC) | ELISA | Humanized IgG1, κ |
MRO-478LC | Human Anti-HCV E2 Protein Recombinant Antibody (MRO-478LC) | ELISA | Humanized IgG1, κ |
MRO-479LC | Human Anti-HCV E2 Protein Recombinant Antibody (MRO-479LC) | ELISA | Humanized IgG1, κ |
MRO-477LC-S(P) | Human Anti-HCV E2 Protein Recombinant Antibody; scFv Fragment (MRO-477LC-S(P)) | ELISA | Humanized scFv |
MRO-478LC-S(P) | Human Anti-HCV E2 Protein Recombinant Antibody; scFv Fragment (MRO-478LC-S(P)) | ELISA | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-485LC-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (AB 68) | ELISA | Human antibody |
MRO-488LC-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (CBH-4D) | ELISA, WB | Human antibody |
MRO-489LC-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (CBH-4B) | ELISA, WB | Human antibody |
MRO-490LC-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (CBH-4G) | ELISA, WB | Human antibody |
MRO-491LC-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (CBH-5) | ELISA, WB | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-1191LC | Anti-HCV E2 Recombinant Antibody (A10F2) | ELISA, Inhib | Mouse Antibody |
MRO-1192LC | Anti-HCV E2 Recombinant Antibody (H6D3) | ELISA, Inhib, WB | Mouse Antibody |
MRO-1193LC | Anti-HCV E2 Recombinant Antibody (E3D8) | ELISA, Inhib, WB | Mouse Antibody |
MRO-1194LC | Anti-HCV E2 Recombinant Antibody (A7A6H9) | ELISA, Inhib | Mouse Antibody |
MRO-1195LC | Anti-HCV E2 Recombinant Antibody (G9E1) | ELISA, Inhib | Mouse Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC428 | PE-A*24:02/HCV E2 (EYVLLLFLL) MHC Tetramer | FCM | |
MHC-LC429 | APC-A*24:02/HCV E2 (EYVLLLFLL) MHC Tetramer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0065LC | Recombinant Mouse Anti-HCV E2 protein Antibody (JS-81) | ELISA | IgG1 |
EPAF-1294LC | Recombinant Human Anti-HCV E2 protein Antibody (4c2) | ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBC-319 | Recombinant Mouse Anti-HCV E2 Antibody scFv Fragment (DAO5) | ELISA, FC | Mouse scFv |
HPAB-1125-CN-S(P) | Recombinant Human Anti-HCV E2 Antibody scFv Fragment (MRCT10.1) | WB, ELISA, IF | Human scFv |
HPAB-1126-CN-S(P) | Recombinant Human Anti-HCV E2 Antibody scFv Fragment (MRCT10.V79) | WB, ELISA, IF | Human scFv |
PSBS-0067 | Human Anti-HCV E2 Recombinant Antibody (Clone HC84-26); scFv Fragment | ELISA, FuncS | Human scFv |
HPAB-1523WJ-S(P) | Human Anti-HCV E2 protein Recombinant Antibody; scFv Fragment (clone A12) | ELISA, WB | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0156-YJ-F(E) | Recombinant Human Anti-HCV E2 Protein Antibody Fab Fragment (e2d066) | ELISA, Inhib | Human Fab |
HPAB-0084-WJ-F(E) | Mouse Anti-HCV E2 Protein Recombinant Antibody; Fab Fragment (HPAB-0084-WJ-F(E)) | ELISA, WB | Mouse Fab |
HPAB-0085-WJ-F(E) | Mouse Anti-HCV E2 Protein Recombinant Antibody; Fab Fragment (HPAB-0085-WJ-F(E)) | ELISA, WB | Mouse Fab |
FAMAB-0198JF-F(E) | Human Anti-HCV E2 Recombinant Antibody (clone L1); Fab Fragment | WB, ELISA, FuncS | Human Fab |
FAMAB-0358JF-F(E) | Human Anti-HCV E2 Recombinant Antibody (clone 9e1); Fab Fragment | ELISA, FuncS | Human Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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