Recombinant Anti-HCV E2 VHH Single Domain Antibody (D03) (CAT#: PNBL-019)

Figure 1 Neutralization activity of sdAbs.

The neutralizing ability of each sdAb (10 lg/mL) was determined using 100 focus-forming units of JFH-1 particles before inoculating Huh7.5 target cells. Neutralization was compared with control neutralizing mAb 1:7 and an irrelevant antitetanus toxin sdAb.

Tarr, A. W., Lafaye, P., Meredith, L., Damier‐Piolle, L., Urbanowicz, R. A., Meola, A., ... & Ball, J. K. (2013). An alpaca sdAb inhibits hepatitis C virus entry and cell‐to‐cell transmission. Hepatology, 58(3), 932-939.

Figure 2 D03 inhibits cell-to-cell transmission of HCV

Transmission between HCV JFH-1–infected Huh-7.5 cells and na€ıve Huh-7.5 cells in the presence of inhibitors was assessed via flow cytometry. Extracellular virus after 24-hour incubation was quantified via end-point titration, and a horizontal black line indicates the number of infected target cells in the presence of an antibody dose that eliminated>95% of cell-free virus. Inhibition of cell-to-cell transmission was defined as reduced infection below this threshold. D03 demonstrated dose-dependent inhibition of direct infection.

Tarr, A. W., Lafaye, P., Meredith, L., Damier‐Piolle, L., Urbanowicz, R. A., Meola, A., ... & Ball, J. K. (2013). An alpaca sdAb inhibits hepatitis C virus entry and cell‐to‐cell transmission. Hepatology, 58(3), 932-939.

Figure 3 Genotype 1 clones show both resistant and sensitive phenotypes.

HCVpp supplemented with genotypes were neutralized by increasing concentrations of mAb nB D03 and the resulting infectivity plotted against antibody concentration.

Urbanowicz, R. A., McClure, C. P., Brown, R. J., Tsoleridis, T., Persson, M. A., Krey, T., ... & Tarr, A. W. (2016). A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance. Journal of virology, 90(7), 3288-3301.

Figure 4 Normally distributed IC50 values for each antibody.

Frequency distribution plots of log10 IC50 values, and the derived Gaussian curve, from all 81 clones for mAb D03. Mean log10 IC50 value for each antibody is represented by a red dashed line, mean ± 2 standard deviations by a solid red line and mean ± 3 standard deviations by the grey fill. Each plot fitted a normal distribution curve as shown by the D'Agostino & Pearson omnibus normality test.

Urbanowicz, R. A., McClure, C. P., Brown, R. J., Tsoleridis, T., Persson, M. A., Krey, T., ... & Tarr, A. W. (2016). A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance. Journal of virology, 90(7), 3288-3301.