Anti-HCV E2 VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Figure 1 Neutralization activity of sdAbs.
The neutralizing ability of each sdAb (10 lg/mL) was determined using 100 focus-forming units of JFH-1 particles before inoculating Huh7.5 target cells. Neutralization was compared with control neutralizing mAb 1:7 and an irrelevant antitetanus toxin sdAb.
Tarr, A. W., Lafaye, P., Meredith, L., Damier‐Piolle, L., Urbanowicz, R. A., Meola, A., ... & Ball, J. K. (2013). An alpaca sdAb inhibits hepatitis C virus entry and cell‐to‐cell transmission. Hepatology, 58(3), 932-939.
Figure 2 D03 inhibits cell-to-cell transmission of HCV
Transmission between HCV JFH-1–infected Huh-7.5 cells and na€ıve Huh-7.5 cells in the presence of inhibitors was assessed via flow cytometry. Extracellular virus after 24-hour incubation was quantified via end-point titration, and a horizontal black line indicates the number of infected target cells in the presence of an antibody dose that eliminated>95% of cell-free virus. Inhibition of cell-to-cell transmission was defined as reduced infection below this threshold. D03 demonstrated dose-dependent inhibition of direct infection.
Tarr, A. W., Lafaye, P., Meredith, L., Damier‐Piolle, L., Urbanowicz, R. A., Meola, A., ... & Ball, J. K. (2013). An alpaca sdAb inhibits hepatitis C virus entry and cell‐to‐cell transmission. Hepatology, 58(3), 932-939.
Figure 3 Genotype 1 clones show both resistant and sensitive phenotypes.
HCVpp supplemented with genotypes were neutralized by increasing concentrations of mAb nB D03 and the resulting infectivity plotted against antibody concentration.
Urbanowicz, R. A., McClure, C. P., Brown, R. J., Tsoleridis, T., Persson, M. A., Krey, T., ... & Tarr, A. W. (2016). A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance. Journal of virology, 90(7), 3288-3301.
Figure 4 Normally distributed IC50 values for each antibody.
Frequency distribution plots of log10 IC50 values, and the derived Gaussian curve, from all 81 clones for mAb D03. Mean log10 IC50 value for each antibody is represented by a red dashed line, mean ± 2 standard deviations by a solid red line and mean ± 3 standard deviations by the grey fill. Each plot fitted a normal distribution curve as shown by the D'Agostino & Pearson omnibus normality test.
Urbanowicz, R. A., McClure, C. P., Brown, R. J., Tsoleridis, T., Persson, M. A., Krey, T., ... & Tarr, A. W. (2016). A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance. Journal of virology, 90(7), 3288-3301.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-118 | Recombinant Rat Anti-HCV E2 Antibody (3/11) | WB, ELISA, Neut, FuncS | IgG |
PABL-119 | Recombinant Human Anti-HCV E2 Antibody (HC33.1) | Neut, FuncS | IgG |
PABL-120 | Recombinant Human Anti-HCV E2 Antibody (HC84-1) | WB, ELISA, Neut, FuncS | IgG |
PABL-121 | Recombinant Mouse Anti-HCV E2 Antibody (HC84-27) | Neut, FuncS | IgG |
PABL-122 | Recombinant Mouse Anti-HCV E2 Antibody (mAb12) | WB, ELISA, FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-040 | Recombinant Mouse Anti-CHIKV E2 glycoprotein Antibody scFv Fragment (5F-10) | Neut, FuncS | scFv |
PSBL-357 | Recombinant Human Anti-VEEV E2 Antibody scFv Fragment (F5) | WB, ELISA, Neut, FuncS | scFv |
PFBC-184 | Recombinant Chimeric (Human/Mouse) Anti-HCV glycoprotein E2 Antibody Fab Fragment (mAb1:7) | Neut | Fab |
HPAB-N0072-YC-S(P) | Recombinant Human Anti-E2 Antibody scFv Fragment (HC84.26.5D) | Neut, ELISA, FuncS | Human scFv |
HPAB-2105-FY-S(P) | Human Anti-E2 Recombinant Antibody (clone D12); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-119 | Recombinant Human Anti-HCV E2 Antibody Fab Fragment (HC33.1) | Neut, FuncS | Fab |
PFBL-120 | Recombinant Human Anti-HCV E2 Antibody Fab Fragment (HC84-1) | WB, ELISA, Neut, FuncS | Fab |
PFBL-121 | Recombinant Mouse Anti-HCV E2 Antibody Fab Fragment (HC84-27) | Neut, FuncS | Fab |
PFBL-122 | Recombinant Mouse Anti-HCV E2 Antibody Fab Fragment (mAb12) | WB, ELISA, FuncS | Fab |
PFBL-123 | Recombinant Mouse Anti-HCV E2 Antibody Fab Fragment (mAb8) | WB, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-238CT | Anti-HCV E2 Recombinant Antibody (Y47F) | ELISA, WB | Human antibody |
TAB-238CT-S(P) | Anti-HCV E2 Recombinant Antibody scFv Fragment (Y47F) | ELISA, WB | Human antibody |
TAB-239CT-S(P) | Anti-HCV E2 Recombinant Antibody scFv Fragment (Y47W) | ELISA, WB | Human antibody |
TAB-238CT-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (Y47F) | ELISA, WB | Human antibody |
TAB-239CT-F(E) | Anti-HCV E2 Recombinant Antibody Fab Fragment (Y47W) | ELISA, WB | Human antibody |
There are currently no Customer reviews or questions for PNBL-019. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.