Anti-HER2/neu Antibody-Cytokine Fusion Protein, IgG (L19)-GM-CSF (CAT#: ACFP-SH047)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-HER2/neu IgG to GM-CSF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the HER2/neu as well as the biological activity of GM-CSF. Pharmacokinetic studies showed that anti-HER2/neu IgG3-(GM-CSF) is stable in the blood. In addition, anti-HER2/neu IgG3-(GM-CSF) was able to target the HER2/neu-expressing tumor and enhance the immune response against the targeted Ag HER2/neu. Anti-HER2/neu IgG3-(GM-CSF) is able to enhance both Th1-and Th2-mediated immune responses. This immunocytokine was designed for treating HER-2/neu over-expressing cancer.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Figure 3 High expression in esophagus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Figure 5 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Figure 6 Kidney
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
(Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Figure 7 Breast
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
Specifications
- Target
- HER2/neu
- Molecule Class
- IgG3-GM-CSF
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- L19
- Related Disease
- HER-2/neu over-expressing cancer
- Cytokine 1
- GM-CSF
- Cytokines 1 Description
- Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2; neuro/glioblastoma derived oncogene homolog
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Downloads
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Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-005 | Anti-Human ErbB2 Recombinant Antibody (Trastuzumab) | FC, IP, ELISA, Neut, FuncS, IF, IHC | IgG1 - kappa |
| TAB-H70 | Anti-Human ERBB2 Recombinant Antibody (Trastuzumab Emtansine) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG1 - kappa |
| TAB-053 | Anti-Human ERBB2 Recombinant Antibody (Pertuzumab) | FuncS, IF, Neut, ELISA, FC, IP, WB | IgG1 - kappa |
| TAB-033CT | Human Anti-ERBB2 Recombinant Antibody (TAB-033CT) | ELISA, WB | Human IgG1, κ |
| TAB-038CT | Human Anti-ERBB2 Recombinant Antibody (TAB-038CT) | FuncS, FC | Humanized IgG1 |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
| TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
| TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-085 | Human Anti-ERBB2 Recombinant Antibody (PABL-085) | ELISA, WB, FuncS | Human IgG |
| PABL-126 | Human Anti-ERBB2 Recombinant Antibody (PABL-126) | ELISA, WB, FuncS | Human IgG |
| PABZ-041 | Human Anti-ERBB2 Recombinant Antibody (clone bH1) | WB, IF, FuncS | Human IgG |
| PABC-157 | Human Anti-ERBB2 Recombinant Antibody (clone F0178C1) | Neut | Human IgG |
| HPAB-0021-YC | Human Anti-ERBB2 Recombinant Antibody (HPAB-0021-YC) | ELISA, Neut, FC, FuncS | Human IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (Gr3) | SPR | Llama VHH |
| PNBL-067 | Recombinant Anti-HER2 VHH Single Domain Antibody (Gr6) | SPR | Llama VHH |
| HPAB-0201-FY | Recombinant llama Anti-ERBB2 Antibody (VHH6) | E | llama VHH |
| HPAB-0202-FY | Recombinant llama Anti-ERBB2 Antibody (VHH1) | E | llama VHH |
| HPAB-0203-FY | Recombinant llama Anti-ERBB2 Antibody | IF | llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-041 | Human Anti-ERBB2 Recombinant Antibody (clone bH1); Fab Fragment | WB, IF, FuncS | Human Fab |
| PFBL-463 | Human Anti-ERBB2 Recombinant Antibody (clone mAb37); Fab Fragment | WB | Human Fab |
| PSBW-161 | Mouse Anti-ERBB2 Recombinant Antibody (clone chA21); scFv Fragment | FC, WB | Mouse scFv |
| FAMAB-0098-YC-F(E) | Mouse Anti-ERBB2 Recombinant Antibody (clone E402); Fab Fragment | Cyt | Mouse Fab |
| FAMAB-0099-YC-F(E) | Human Anti-ERBB2 Recombinant Antibody (clone CH402); Fab Fragment | Cyt | Human Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0193CL-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-0193CL-F(E)) | ELISA, Cyt, Internalization | Human Fab |
| TAB-0194CL-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-0194CL-F(E)) | ELISA, Cyt, Internalization | Human Fab |
| TAB-055CT | Anti-Human HER2/neu Recombinant Antibody | WB | Human antibody |
| TAB-040CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (C6.5) | ELISA, WB | Human antibody |
| PABX-081-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr3) | WB, ELISA, FuncS | scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034CT | Anti-Human HER2/neu Recombinant Antibody (pertuzumab ) | WB | |
| TAB-036CT | Anti-Human HER2/neu Recombinant Antibody (6B2) | ELISA, WB | |
| TAB-270CQ-S(P) | Mouse Anti-ERBB2 Recombinant Antibody; scFv Fragment (TAB-270CQ-S(P)) | WB, Inhib | Mouse scFv |
| TAB-270CQ-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-270CQ-F(E)) | WB, Inhib | Mouse Fab |
| PABX-082-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr6) | WB, ELISA, FuncS | scFv |
Fab Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-118LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
| Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2956 | APC-A*03:01/Human ERBB2 (VLRENTSPK) MHC Tetramer | FCM | |
| MHC-LC2957 | APC-A*11:01/Human ERBB2 (VLRENTSPK) MHC Tetramer | FCM | |
| MHC-LC2958 | APC-A*31:01/Human ERBB2 (VLRENTSPK) MHC Tetramer | FCM | |
| MHC-YF221 | A*02:01/Human Her-2/neu (KIFGSLAFL) MHC Monomer | MHC Multimer | |
| MHC-YF222 | A*02:01/Human Her-2/neu (RLLQETELV) MHC Monomer | MHC Multimer |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1333WJ-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone 3.F2); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-1334WJ-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone 1.D2); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-S0027-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone F5); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
| HPAB-S0028-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C1); scFv Fragment | ELISA, Inhib, Internalization | Human scFv |
| HPAB-1855-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-1855-FY-F(E)) | ELISA | Humanized Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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