Recombinant monoclonal antibody to HER2/neu. This antibody intended for the prophylaxis and treatment of ovarian carcinoma xenografts.
Figure 1 Expression and identification of fused proteins.
Western blot analysis of purified proteins. 1: MIL5scFv; lane 2: MIL5scFv-Arg9. The predicted molecular weight of MIL5scFv-Arg9 was about 49 kDa, which was almost the same with that of MIL5scFv.
Hu, Y., Qiao, C., Lv, M., Feng, J., Yu, M., Shen, B., ... & Li, Y. (2012). Arg9 facilitates the translocation and downstream signal inhibition of an anti-HER2 single chain antibody. BMC research notes, 5(1), 336.
Figure 2 Flow cytometry analysis and confocal microscopy observation of MIL5scFv-Arg9 to penetrate NIH3T3 cells (HER2 negative).
Cells treated with MIL5scFv-FITC. The intensity of fluorescence signal was evaluated with MFI value marked on the right top of each panel. According to the fluorescence intensity, Arg9 could help MIL5scFv penetrate NIH3T3 cells. Data are representative of two independent experiments.
Hu, Y., Qiao, C., Lv, M., Feng, J., Yu, M., Shen, B., ... & Li, Y. (2012). Arg9 facilitates the translocation and downstream signal inhibition of an anti-HER2 single chain antibody. BMC research notes, 5(1), 336.
Figure 3 Flow cytometry analysis and confocal microscopy observation of MIL5scFv-Arg9 to penetrate NIH3T3 cells (HER2 negative).
Confocal microscopy observation of MIL5scFv-Arg9 to penetrate NIH3T3 cells. Cells were incubated with PBS (Control) or herceptin-FITC as negative control, MIL5scFv-Arg9-FITC (1:10) or MIL5scFv-FITC (1:10) at 37°C and collected consecutively in 0.5 h, 1 h, 2 h and 5 h. The distribution of fluorescence signal in the cells was observed under a laser scanning confocal microscopy. Fluorescent signal could be detected after 0.5 hour in MIL5scFv-Arg9 treated samples, while in MIL5scFv treated samples, weak signal could be seen after 5 hours. Each panel was merged with two photos: FITC conjugated protein (green) and nuclei (blue, cells were stained with Hoechst 33258), which were not shown respectively here.
Hu, Y., Qiao, C., Lv, M., Feng, J., Yu, M., Shen, B., ... & Li, Y. (2012). Arg9 facilitates the translocation and downstream signal inhibition of an anti-HER2 single chain antibody. BMC research notes, 5(1), 336.
Figure 4 Enhanced translocation and stronger activity of MIL5scFv-Arg9 to downregulate phospho-Akt in SKOV3 cells (HER2 positive).
Densities of phosphor-Akt were normalized based on densities of Akt shown in Figure 4B, suggesting satisfactory enhanced effect of Arg9 in inhibiting the expression level of phospho-Akt in SKOV3 cells. The data shown are calculate from two independent experiments.
Hu, Y., Qiao, C., Lv, M., Feng, J., Yu, M., Shen, B., ... & Li, Y. (2012). Arg9 facilitates the translocation and downstream signal inhibition of an anti-HER2 single chain antibody. BMC research notes, 5(1), 336.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-059 | Recombinant Anti-Human ERBB2 VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
HPAB-0201-FY | Recombinant llama Anti-ERBB2 Antibody (VHH6) | E | llama VHH |
HPAB-0202-FY | Recombinant llama Anti-ERBB2 Antibody (VHH1) | E | llama VHH |
HPAB-0203-FY | Recombinant llama Anti-ERBB2 Antibody | IF | llama VHH |
HPAB-1026WJ | Recombinant Camelid Anti-ERBB2 Single Domain Antibody | ELISA | Camelid VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
TAB-049CT-F(E) | Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H70 | Anti-Human ERBB2 Recombinant Antibody (Trastuzumab Emtansine) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG1 - kappa |
TAB-053 | Anti-Human ERBB2 Recombinant Antibody (Pertuzumab) | FuncS, IF, Neut, ELISA, FC, IP, WB | IgG1 - kappa |
TAB-033CT | Human Anti-ERBB2 Recombinant Antibody (TAB-033CT) | ELISA, WB | Human IgG1, κ |
TAB-038CT | Human Anti-ERBB2 Recombinant Antibody (TAB-038CT) | FuncS, FC | Humanized IgG1 |
TAB-039CT | Anti-Human HER2/neu Recombinant Antibody (h1E11) | WB | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-126 | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (PFBL-126) | ELISA, WB, FuncS | Human Fab |
PFBZ-041 | Human Anti-ERBB2 Recombinant Antibody (clone bH1); Fab Fragment | WB, IF, FuncS | Human Fab |
PFBL-463 | Human Anti-ERBB2 Recombinant Antibody (clone mAb37); Fab Fragment | WB | Human Fab |
HPAB-0021-YC-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0021-YC-F(E)) | ELISA, Neut, FC | Human Fab |
HPAB-0010-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-0010-FY-F(E)) | ELISA, FC | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-055CT | Anti-Human HER2/neu Recombinant Antibody | WB | Human antibody |
TAB-056CT | Human Anti-ERBB2 Recombinant Antibody (TAB-056CT) | WB | Human IgG |
TAB-056CT-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-056CT-F(E)) | WB | Human Fab |
PABX-081-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr3) | WB, ELISA, FuncS | scFv |
PABX-081-F (E) | Recombinant Human Anti-HER2 Antibody Fab Fragment (Gr3 ) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
Gly-039LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-117LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
Gly-118LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Mouse antibody |
Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
BRD-0695MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
BRD-0779MZ | Chicken Anti-ErbB2 Polyclonal IgY | ICC, IF, IHC, IP | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC3036 | PE-A*24:02/Human ERBB2 (SYGVTVWEL) MHC Tetramer | FCM | |
MHC-LC3037 | PE-A*24:02/Human ERBB2 (VYMIMVKCW) MHC Tetramer | FCM | |
MHC-LC3050 | PE-A*02:01/Human ERBB2 (ALCRWGLLL) MHC Tetramer | FCM | |
MHC-YF049 | DRB1*01:01/Human HER2 (KVPIKWMALESILRRRF) MHC Monomer | MHC Multimer | |
MHC-YF055 | DRB1*04:01/Human HER2 (KVPIKWMALESILRRRF) MHC Monomer | MHC Multimer |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0098YC | Mouse Anti-ERBB2 Recombinant Antibody (clone E402) | Cyt | Mouse IgG1, κ |
FAMAB-0099YC | Human Anti-ERBB2 Recombinant Antibody (clone CH402) | Cyt | Chimeric (mouse/human) IgG1 |
HPAB-0193-WJ | Mouse Anti-ERBB2 Recombinant Antibody (clone 6H8) | ELISA | Mouse IgG1 |
HPAB-AP082-YC | Human Anti-ERBB2 Recombinant Antibody (HPAB-AP082-YC) | ELISA, FuncS | Human IgG |
HPAB-AP083-YC | Human Anti-ERBB2 Recombinant Antibody (HPAB-AP083-YC) | ELISA, FuncS | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1525-FY-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-1525-FY-F(E)) | ELISA | Humanized Fab |
HPAB-1265WJ-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-1265WJ-S(P)) | ELISA | Humanized scFv |
FAMAB-0173JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone C6H211); scFv Fragment | ELISA, FuncS | Human scFv |
FAMAB-0359JF-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone FRc13); scFv Fragment | DB, ELISA, FuncS | Human scFv |
PSBS-0070 | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (PSBS-0070) | ELISA, FuncS | Human scFv |
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