Human Anti-HIV-1 gp120 Recombinant Antibody (clone N5-i5) (CAT#: PABX-100)

Anti-HIV-1 antibody is a Human antibody of IgG class that binds to an HIV-1.


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Figure 1 ADCC assay using cells infectedwithHIV-1 BaL IMC.

Figure 1 ADCC assay using cells infectedwithHIV-1 BaL IMC.

EGFP-CEM-NKr-CCR5-SNAP cellswere spinoculatedwith HIV-1 Balmolecular clone at 2000 rpmfor 2 h at 12 °C.

Orlandi, C., Flinko, R., & Lewis, G. K. (2016). A new cell line for high throughput HIV-specific antibody-dependent cellular cytotoxicity (ADCC) and cell-to-cell virus transmission studies. Journal of immunological methods, 433, 51-58.

Figure 2 Flow cytometry analysis.

Figure 2 Flow cytometry analysis.

After 5 days of co-culturewith the virus, cellswerewashed twice, labeled with Alexa Fluor 647-SNAP tag dye and incubated with dilutions ofmAbs (C11, N5-i5,N10-U1 or Synagis) for 15min at RT, then PBMC were added to the reaction for 3 h at 37 °C. At the end of the incubation, the samples were washed with PBS, fixed with 1% paraformaldehyde and analyzed by flow cytometry. The ADCC data represent the typical results obtained in three independent experiments. Upper right panel. The efficiency of the infection was evaluated by staining of the cells with live/dead (not shown), CD4-APC and p24-PE. Lower right panel. Binding of infected EGFP-CEM-NKr-CCR5-SNAP cells with 5 μg/ml Alexa Fluor-647-conjugated mAbs C11 (panel A), N5-i5 (panel B) or N10U1 (panel C).

Orlandi, C., Flinko, R., & Lewis, G. K. (2016). A new cell line for high throughput HIV-specific antibody-dependent cellular cytotoxicity (ADCC) and cell-to-cell virus transmission studies. Journal of immunological methods, 433, 51-58.

Figure 3 Crystal structures of the N5-i5 Fab-gp12093TH057 coree-d1d2CD4 and 2.2c Fab-gp12089.6P coree-d1d2CD4 complexes.

Figure 3 Crystal structures of the N5-i5 Fab-gp12093TH057 coree-d1d2CD4 and 2.2c Fab-gp12089.6P coree-d1d2CD4 complexes.

The light/heavy chains of N5-i5 Fab and 2.2c Fab are shown in light blue/dark blue and light pink/dark pink, respectively, and the complementarity-determining regions (CDRs) are shown in black (CDR L1), brown (CDR L2), light blue (CDR L3), gray (CDR H1), green (CDR H2), and cyan (CDR H3). The gp120 inner domain is shown in yellow, and the outer domain is in orange. See also Fig. S1A in the supplemental material.

Acharya, P., Tolbert, W. D., Gohain, N., Wu, X., Yu, L., Liu, T., ... & Luongo, T. S. (2014). Structural definition of an antibody-dependent cellular cytotoxicity response implicated in reduced risk for HIV-1 infection. Journal of virology, 88(21), 12895-12906.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Tested positive against HIV-1
  • Species Reactivity
  • HIV-1
  • Clone
  • N5-i5
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS (Assay-dependent)

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Assay-dependent

Target

  • Alternative Names
  • HIV-1; human immunodeficiency virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Recombinant Antibody

Single-domain Antibody

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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