Recombinant Human Anti-HIV-1 Antibody scFv Fragment (447-52D) (CAT#: PABX-088-S (P))

Anti-HIV-1 antibody is a Human of scFv class that binds to an HIV-1. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date.


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Figure 1 Binding of the mutated 447-52D IgG to the indicated gp142 trimers as measured by ELISA.

Figure 1 Binding of the mutated 447-52D IgG to the indicated gp142 trimers as measured by ELISA.

McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.

Figure 2 The area under the curve of the Ca2 flux response (determined in panel C) through the mutated 447-52D BCR correlates with the affinity of sIgG 447-54D (determined in panel A).

Figure 2 The area under the curve of the Ca2 flux response (determined in panel C) through the mutated 447-52D BCR correlates with the affinity of sIgG 447-54D (determined in panel A).

McGuire, A. T., Glenn, J. A., Lippy, A., & Stamatatos, L. (2014). Diverse recombinant HIV-1 Envs fail to activate B cells expressing the germline B cell receptors of the broadly neutralizing anti-HIV-1 antibodies PG9 and 447-52D. Journal of virology, 88(5), 2645-2657.

Figure 3 Neutralization of chimeric psVs by antibodies 447-52D (purple) and 2221 (blue).

Figure 3 Neutralization of chimeric psVs by antibodies 447-52D (purple) and 2221 (blue).

The psV consist of the SF162 strain with its V3 loop replaced by the indicated V3 loop sequence. Consensus B-R18Q is a psV consisting of the consensus subtype B V3 loop sequence with position 18 in the V5 loop mutated from a subtype B consensus arginine t

Almond, D., Krachmarov, C., Swetnam, J., Zolla-Pazner, S., & Cardozo, T. (2012). Resistance of subtype C HIV-1 strains to anti-V3 loop antibodies. Advances in virology, 2014.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • scFv
  • Specificity
  • Tested positive against native DENV-1
  • Species Reactivity
  • Human immunodeficiency virus 1
  • Clone
  • 447-52D
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    IF 1:100 -1:500
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • HIV-1; human immunodeficiency virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NAB-1889-sdAb Recombinant Anti-HIV-1 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH
PNBL-038 Recombinant Anti-HIV-1 C186 gp120 VHH Single Domain Antibody (A12) WB, FuncS Llama VHH

Humanized Antibody

Immunotoxin

CAT Product Name Application Type
AGTO-G037E Anti-HIV-1 immunotoxin 3B3 (scFv)-PE Cytotoxicity assay, Function study
AGTO-G037D Anti-HIV-1 immunotoxin 3B3 (scFv)-DT Cytotoxicity assay, Function study

Chimeric Antibody

CAT Product Name Application Type
PABX-093-S (P) Recombinant Mouse Anti-HIV-1 Antibody scFv Fragment (Fab28) WB, ELISA, FuncS scFv

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4221 Rabbit Anti-HIV-1 Recombinant Antibody (clone SI300DS) ELISA, ICC, IF, FC Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-068 Afuco™ Anti-HIV-1 ADCC Recombinant Antibody (Suvizumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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