Recombinant Human Anti-HIV-1 Antibody scFv Fragment (Fab2219) (CAT#: PABX-091-S (P))

Anti-HIV-1 antibody is a Human of scFv class that binds to an HIV-1. This antibody was originally selected for binding to a V3 fusion protein and can neutralize primary isolates from subtypes B, A, and F. Thus, it represents a cross-reactive, human anti-V3 antibody. It developed From a human immunodeficiency virus type 1-infected individual.


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Figure 1 Measurement of Env incorporation by Western blot.

Figure 1 Measurement of Env incorporation by Western blot.

WT and mutant viruses were produced in transfected 293T cells, lysed, and analyzed by SDS-PAGE (4±20%) and Western blot. An anti-gp120 MAb cocktail (V3: 391/95-D, 694/98-D, 2219, 2558; C2: 847-D, 1006-30D; C5: 450-D, 670-D) and a p24 Gag MAb (91±5) were used to detect the relative levels of Env and Gag associated with virions. The ratios of Env/Gag were calculated.

Upadhyay, C., Feyznezhad, R., Yang, W., Zhang, H., Zolla-Pazner, S., & Hioe, C. E. (2018). Alterations of HIV-1 envelope phenotype and antibody-mediated neutralization by signal peptide mutations. PLoS pathogens, 14(1), e1006812.

Figure 2 Reactivities of MAbs with V3 peptides by ELISA.

Figure 2 Reactivities of MAbs with V3 peptides by ELISA.

The binding curves for anti-V3 MAbs 2219 and 447-52D are shown with filled squares and triangles, respectively. Data for the human anti-parvovirus B19 MAb 1418, used as a negative control, are shown as open circles. O.D., optical density; *, value obtained by extrapolation because the binding curve did not reach the saturation point; NR, not reactive.

Stanfield, R. L., Gorny, M. K., Zolla-Pazner, S., & Wilson, I. A. (2006). Crystal structures of human immunodeficiency virus type 1 (HIV-1) neutralizing antibody 2219 in complex with three different V3 peptides reveal a new binding mode for HIV-1 cross-reactivity. Journal of virology, 80(12), 6093-6105.

Figure 3 The neutralization of nine HIV-1 pseudoviruses by mAb 2191 using U87 as target cells.

Figure 3 The neutralization of nine HIV-1 pseudoviruses by mAb 2191 using U87 as target cells.

The neutralization curves of anti-V3 mAb 2191 against nine selected HIV-1 pseudoviruses are shown with their corresponding AUC and IC50 values. Fifty percent neutralization is denoted
by the dashed line. Significant neutralization at the confidence level of p,0.001 (denoted with * after AUC values) was determined statistically based on comparison with the AUC values of the negative controls together with the slopes of the titration curves as described in Materials and Methods.
For viruses coming from patients where the date of infection is known, viruses are denoted as coming from acutely- or chronically-infected patients,
and the clade of the virus is denoted by the capital letter in its name (A, B, C or D).
doi:10.1371/journal.pone.0010254.g001

Hioe, C. E., Wrin, T., Seaman, M. S., Yu, X., Wood, B., Self, S., ... & Zolla-Pazner, S. (2010). Anti-V3 monoclonal antibodies display broad neutralizing activities against multiple HIV-1 subtypes. PloS one, 5(4), e10254.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • scFv
  • Specificity
  • Tested positive against native E. coli DsbB
  • Species Reactivity
  • Human immunodeficiency virus 1
  • Clone
  • Fab2219
  • Applications
  • ELISA 1:10000-1:20000
    Neut(assay-depended)
    FuncS(assay-depended)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • HIV-1; human immunodeficiency virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NAB-1889-sdAb Recombinant Anti-HIV-1 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH
PNBL-038 Recombinant Anti-HIV-1 C186 gp120 VHH Single Domain Antibody (A12) WB, FuncS Llama VHH

Humanized Antibody

Immunotoxin

CAT Product Name Application Type
AGTO-G037E Anti-HIV-1 immunotoxin 3B3 (scFv)-PE Cytotoxicity assay, Function study
AGTO-G037D Anti-HIV-1 immunotoxin 3B3 (scFv)-DT Cytotoxicity assay, Function study

Chimeric Antibody

CAT Product Name Application Type
PABX-093-S (P) Recombinant Mouse Anti-HIV-1 Antibody scFv Fragment (Fab28) WB, ELISA, FuncS scFv

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4221 Rabbit Anti-HIV-1 Recombinant Antibody (clone SI300DS) ELISA, ICC, IF, FC Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-068 Afuco™ Anti-HIV-1 ADCC Recombinant Antibody (Suvizumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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