Anti-HIV-1 gp120 Recombinant Antibody (CH08) (CAT#: MRO-149LC)

This product is a recombinant human anti-HIV-1 monoclonal antibody. CH08 specifically binds to gp120 and can be potentially used in the treatment of acquired immunodeficiency syndrome (AIDS), a condition in humans characterized by clinical features including wasting syndromes, central nervous system degeneration and profound immunosuppression that results in life-threatening opportunistic infections and malignancies.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA
Neut

Figure 1 HIV-1 pseudovirus neutralization by mAbs CH07 and CH08.

Figure 1 HIV-1 pseudovirus neutralization by mAbs CH07 and CH08.

A panel of tier 1 and tier 2 HIV-1 pseudoviruses were tested against mAbs CH07, CH08, 17b, and 412-D in TZM-bl cells. CH07 weakly neutralized a single virus (92TH023.CRF01_AE). In contrast, CH08 neutralized multiple tier 1 and tier 2 viruses in a pattern similar to that of CD4i mAbs 17b and 412-D. A fragment (Fab) of CH08 neutralized with a potency similar to that of the intact mAb, suggesting that the binding site for mAb CH08 was not inaccessible for most of the isolates tested. An additional panel of 13 tier 2 viruses was not neutralized by any mAb tested.

Isolation of HIV-1-Neutralizing Mucosal Monoclonal Antibodies from Human Colostrum

ADCC

Figure 2 ADCC and virus capture by mAbs CH07 and CH08.

Figure 2 ADCC and virus capture by mAbs CH07 and CH08.

CH07 and CH08 were tested for ADCC activity compared with anti-RSV mAb (Palivizumab) and A32. CH07 did not mediate ADCC, while both A32 and CH08 stimulated granzyme B activity NK effector cells when reacted with infected and gp120-coated targets. Each bar represents the percent of target cells containing granzyme B.

Isolation of HIV-1-Neutralizing Mucosal Monoclonal Antibodies from Human Colostrum

SPR

Figure 3 MAb CH08 is a CD4i HIV Env-specific antibody.

Figure 3 MAb CH08 is a CD4i HIV Env-specific antibody.

CH08 mAb binding to gp140Con.S after capture by mAb T8 (HIV gp120-specific mAb), soluble CD4 (sCD4), or mAb A32 (CD4 binding site mAb) was measured by SPR.

Isolation of HIV-1-Neutralizing Mucosal Monoclonal Antibodies from Human Colostrum


Specifications

  • Host Species
  • Human
  • Type
  • Human antibody
  • Specificity
  • HIV-1
  • Clone
  • CH08
  • Applications
  • ELISA, Neut, ADCC, SPR
  • Related Disease
  • AIDS

Applications

  • Application Notes
  • The HIV-1 gp120 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization, Antibody-dependent Cell-mediated Cytotoxicity, Surface Plasmon Resonance.

Target

  • Alternative Names
  • Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP120; gp120; HIV envelope

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone CH08"

See other products for "HIV-1 gp120"

Single-domain Antibody

Chimeric Antibody

CAT Product Name Application Type
MRO-135LC Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) ELISA, WB, Neut Chimeric antibody (mouse/human)
MRO-135LC-S(P) Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)
MRO-135LC-F(E) Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) ELISA, WB Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4220 Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) WB, ELISA Rabbit IgG

Humanized Antibody

CAT Product Name Application Type
PABX-101-S(P) Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment WB, ELISA, FuncS Human scFv

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for MRO-149LC. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare