Human Anti-HIV-1 gp120 Recombinant Antibody (clone F425-B4e8) (CAT#: PABZ-064)
Recombinant Human Antibody (F425-B4e8) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG2 and a light chain (LC) encoding VL from anti-HIV-1 gp120 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. Human mAb B4e8 neutralizes a subset of viruses from HIV-1 subtypes B, C and D.
Specific Inquiry
Figure 1 Competition of PG9 with antibodies against the V2 loop (10/76b), V3 loop (F425/b4e8), and the CD4i site (X5) for gp120 binding.
Antigens were coated directly onto ELISA wells in the experiments shown, but similar results were also obtained when antigens were captured by antibodies against noncompetitive epitopes.
Walker, L. M., Phogat, S. K., Chan-Hui, P. Y., Wagner, D., Phung, P., Goss, J. L., ... & Lehrman, J. K. (2009). Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine target. Science, 326(5950), 285-289.
Figure 2 Comparison of the neutralization breadth of mAbs F425-B4e8 and 447-52D
IC₅₀ values below 50 μg/ml are denoted in bold type.
Pantophlet, R., Aguilar-Sino, R. O., Wrin, T., Cavacini, L. A., & Burton, D. R. (2007). Analysis of the neutralization breadth of the anti-V3 antibody F425-B4e8 and re-assessment of its epitope fine specificity by scanning mutagenesis. Virology, 364(2), 441-453.
Specifications
- Host Species
- Human
- Type
- Human IgG2, κ
- Specificity
- Tested positive against HIV-1 gp120
- Species Reactivity
- HIV-1
- Clone
- F425-B4e8
- Applications
- ELISA Assay-Dependent
Neut Assay-Dependent
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody F425-B4e8 has been reported in applications of ELISA, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-135 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT135) | ELISA, Neut | Human IgG |
| PABL-142 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 412d) | Neut, WB, ELISA, FuncS | Human IgG |
| PABL-155 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC03) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-156 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone VRC-CH31) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-160 | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 59.1) | WB, ELISA, FuncS | Mouse IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-164 | Human Anti-HIV-1 gp120 Recombinant Antibody; scFv Fragment (PSBZ-164) | Neut | Human scFv |
| PSBZ-165 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone GL-VRC01); scFv Fragment | Neut | Human scFv |
| PSBZ-209 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 19.3H-L3); scFv Fragment | Neut | Human scFv |
| PSBZ-210 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 19.3H-L1); scFv Fragment | Neut | Human scFv |
| MRO-3081CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH276); Fab Fragment | Neut | Human Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
| MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
| MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
| MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
| MRO-032LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91H9) | ELISA | Single domain antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
| MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
| MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
| MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
| MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
| MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
| MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-041LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (D59/A2) | ELISA | Mouse antibody |
| MRO-042LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (F58/H3) | ELISA | Mouse antibody |
| MRO-043LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P1/D12) | ELISA | Mouse antibody |
| MRO-044LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P4/D10) | ELISA | Mouse antibody |
| MRO-045LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (A47/B1) | ELISA | Mouse antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-3410CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 1.9E); scFv Fragment | ELISA | Human scFv |
| MRO-3411CQ-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 1.9F); scFv Fragment | ELISA | Human scFv |
| MRO-3647CQ-S(P) | Rat Anti-HIV-1 gp120 Recombinant Antibody (clone 11/68b); scFv Fragment | Neut | Rat scFv |
| MRO-3648CQ-S(P) | Rat Anti-HIV-1 gp120 Recombinant Antibody (clone 41.1 (ICR41.1i, ICR41. ICR 41.1i)); scFv Fragment | Neut | Rat scFv |
| MRO-3649CQ-S(P) | Rat Anti-HIV-1 gp120 Recombinant Antibody (clone 62c); scFv Fragment | ELISA | Rat scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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