Recombinant Human Antibody (GL-VRC01) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-HIV-1 gp120 proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. VRC01-class bNAbs all derive from the human VH1-2*02 variable heavy gene, but differ significantly in amino acid sequence and complementarity-determining region H3 (CDRH3) length and use a few different variable light chain genes.
Figure 1 Binding of GL and mature (Mat) antibodies to gp120 and eOD variants.
Jardine, J., Julien, J. P., Menis, S., Ota, T., Kalyuzhniy, O., McGuire, A., ... & Nieusma, T. (2013). Rational HIV immunogen design to target specific germline B cell receptors. Science, 1234150.
Figure 2 Generation of VRC01 gH mice and outline of priming experiments.
(A) Flow cytometry analysis of spleen cells showing B cell frequencies in VRC01 gH mice and WT littermates. (B) Next-generation sequencing of splenic cDNA from VRC01 gH mice revealed VH1-2*02 usage compared with mouse VH gene usage. (C) Summary of the time course for experiments and analysis. Mice were given a single prime of immunogen in adjuvant and then serum immune responses were evaluated at days 14, 28, and 42 postimmunization. Two of five mice per group were sacrificed for B cell sorting analysis at day 14, and the remaining three of five mice were sacrificed at day 42. In other animals, splenic B cells were collected at days 5, 10, or 31 for hybridoma generation. (D) Overview of the immunization groups listed by immunogen (eOD-GT8 60mer, eOD-GT8 3mer, eOD17 60mer, and BG505 SOSIP), multimeric state (nanoparticle or trimer), and adjuvant (Alum, Iscomatrix, or Ribi), along with the number of mice used to test each group. All groups were tested in both VRC01 gH and WTmice, except for BG505 SOSIP, which was tested only in VRC01 gH mice. Five mice per group were used for B cell sorting and/or ELISA, and an additional 13 VRC01 gH mice were employed for hybridoma generation after immunization with eOD-GT8 60mer (6 for Alum, 7 for Ribi).
Jardine, J. G., Ota, T., Sok, D., Pauthner, M., Kulp, D. W., Kalyuzhniy, O., ... & Menis, S. (2015). Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen. Science, 349(6244), 156-161.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
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• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-082 | Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
MRO-028LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91C2) | ELISA | Single domain antibody |
MRO-029LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91F10) | ELISA | Single domain antibody |
MRO-030LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91E1) | ELISA | Single domain antibody |
MRO-031LC-VHH | Anti-HIV-1 gp120 Recombinant Antibody (L91B5) | ELISA | Single domain antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-164 | Human Anti-HIV-1 gp120 Recombinant Antibody; scFv Fragment (PSBZ-164) | Neut | Human scFv |
PSBZ-165 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone GL-VRC01); scFv Fragment | Neut | Human scFv |
PSBZ-209 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 19.3H-L3); scFv Fragment | Neut | Human scFv |
PSBZ-210 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone 19.3H-L1); scFv Fragment | Neut | Human scFv |
FAMAB-0177CQ-F(E) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone BAT123); Fab Fragment | Neut | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-532 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT122); scFv Fragment | Neut | Human scFv |
PSBL-533 | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PGT123); scFv Fragment | Neut | Human scFv |
PSBL-534 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone R20); scFv Fragment | WB, FuncS | Rabbit scFv |
MRO-3594CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 5E11); scFv Fragment | ELISA | Mouse scFv |
MRO-3595CQ-S(P) | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 7H3); scFv Fragment | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-125LC | Anti-HIV-1 gp120 Recombinant Antibody (N6) | Neut, ELISA | Human antibody |
MRO-130LC | Human Anti-HIV-1 gp120 Recombinant Antibody (MRO-130LC) | ELISA, Neut, SPR | Human IgG |
MRO-136LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900973) | ELISA | Human antibody |
MRO-137LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900972) | ELISA | Human antibody |
MRO-138LC | Anti-HIV-1 gp120 Recombinant Antibody (Ab900990) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-135LC | Anti-HIV-1 gp120 Recombinant Antibody (CAG1-51-4) | ELISA, WB, Neut | Chimeric antibody (mouse/human) |
MRO-135LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
MRO-135LC-F(E) | Anti-HIV-1 gp120 Recombinant Antibody Fab Fragment (CAG1-51-4) | ELISA, WB | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-041LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (D59/A2) | ELISA | Mouse antibody |
MRO-042LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (F58/H3) | ELISA | Mouse antibody |
MRO-043LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P1/D12) | ELISA | Mouse antibody |
MRO-044LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (P4/D10) | ELISA | Mouse antibody |
MRO-045LC-S(P) | Anti-HIV-1 gp120 Recombinant Antibody scFv Fragment (A47/B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4220 | Rabbit Anti-HIV-1 gp120 Recombinant Antibody (clone SI299DS) | WB, ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-3638CQ | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 9305) | Neut | Mouse IgG |
MRO-3639CQ | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone CRA-6 (CRA6)) | ELISA | Mouse IgG |
MRO-3640CQ | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone Chessie 6) | ELISA, WB | Mouse IgG |
MRO-3641CQ | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 101-342) | ELISA, WB | Mouse IgG2a, κ |
MRO-3642CQ | Mouse Anti-HIV-1 gp120 Recombinant Antibody (clone 101-451) | ELISA, WB | Mouse IgG2b, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-101-S(P) | Human Anti-HIV-1 gp120 Recombinant Antibody (clone PG9); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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