Human Anti-HIV-1 gp41 Recombinant Antibody (clone 13H11) (CAT#: PABL-190)

Figure 1 Comparison of binding of 2F5 and 13H11 Fabs to GCN4-gp41-inter, a mimic of pre-hairpin intermediate structure of gp41.

Dennison, S. M., Sutherland, L. L., Jaeger, F. H., Anasti, K. M., Parks, R., Stewart, S.,... & Scearce, R. M. (2011). Induction of antibodies in rhesus macaques that recognize a fusion-intermediate conformation of HIV-1 gp41. PloS one,6(11), e27824.

Figure 2 The JRFL gp140CF primed and MPER656 liposomes boosted rhesus macaque serum IgG bind scaffold proteins containing engrafted MPER in the 2F5 bound conformation.

A–C: SPR sensogram of 2F5, 11F10 and 13H11 mAbs at the indicated concentrations binding to ES2 (A), ES4 (B), and ES5 (C) scaffolds respectively are shown. D–F: The post MPER656 liposome immune serum IgG of rhesus macaques (150 µg/ml) binding to ES2 (D), ES4 (E), ES5 (F) are shown. For the sake of clarity, the scaffold binding responses of pre-MPER656 liposome serum IgG of only one animal (# 41546) is shown. The responses from other animal serum IgG were similar.

Dennison, S. M., Sutherland, L. L., Jaeger, F. H., Anasti, K. M., Parks, R., Stewart, S.,... & Scearce, R. M. (2011). Induction of antibodies in rhesus macaques that recognize a fusion-intermediate conformation of HIV-1 gp41. PloS one,6(11), e27824.

Figure 3 Binding of MAbs 2F5, 13H11, and 5A9 to HIV-1 MPER peptides. Biotinylated full-length HR-2 peptide, SP62, or 8926 was immobilized at 200 to 300 RU on a streptavidin (SA) sensor chip. Biotin-DP107 was used as a control to subtract nonspecific binding. Representative overlay of specific (control subtracted) binding curves show binding of each of the MAbs to HR-2 (solid line), SP62 (dotted line), or 8926 (broken line) peptides.

Alam, S. M., Scearce, R. M., Parks, R. J., Plonk, K., Plonk, S. G., Sutherland, L. L.,... & Xia, S. M. (2008). Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection.Journal of virology, 82(1), 115-125.

Figure 4 Cross-blocking of 2F5 and 13H11 MAb binding to HR-2 peptide.

Alam, S. M., Scearce, R. M., Parks, R. J., Plonk, K., Plonk, S. G., Sutherland, L. L.,... & Xia, S. M. (2008). Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection.Journal of virology, 82(1), 115-125.

Figure 5 Ability of plasma from 75 chronically HIV-1-positive patients (CD4 counts of >400; no antiretroviral therapy) and HIV-1-negative control plasma from 25 healthy donors to inhibit either the biotinylated MAb 2F5 or MAb 13H11. Twenty of 50 plasma samples from the chronically HIV-1-positive patients inhibited MAb 2F5 binding at ≥20% levels, while 62 of 75 plasma samples from the chronically HIV-1-positive patients inhibited MAb 13H11 binding at ≥20% levels. No significant inhibition of either MAb 2F5 or 13H11 binding by HIV-1-negative control plasma (n = 50) was observed. (B) Ability of plasma from 20 acutely HIV-1-infected patients and HIV-1-negative control plasma from 26 healthy donors to inhibit either the biotinylated MAb 2F5 or MAb 13H11. Two of 20 plasma samples from the acutely HIV-1-infected patients inhibited MAb 2F5 binding at ≥20% levels, while 19 of 20 plasma samples from the acutely HIV-1-infected patients inhibited MAb 13H11 binding at ≥20% levels. No significant inhibition of either MAb 2F5 or 13H11 binding by HIV-1-negative control plasma (n = 26) was observed.

Alam, S. M., Scearce, R. M., Parks, R. J., Plonk, K., Plonk, S. G., Sutherland, L. L.,... & Xia, S. M. (2008). Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection.Journal of virology, 82(1), 115-125.

Figure 6 Antibody responses to HIV-1 Env MPER epitopes in acutely HIV-1-infected patients.

Serial plasma samples were collected from 12 individuals from the clade C CAPRISA cohort from the initial study point through approximately 70 weeks, as shown on the x axis, and assayed for their ability to block the binding of biotinylated MAbs 2F5 or 13H11 to HIV-1 JRFL gp140 Env in ELISA. Mean values (n = 12) of microgram equivalents of either 2F5 or 13H11 antibody in plasma are shown on the y axis.

Alam, S. M., Scearce, R. M., Parks, R. J., Plonk, K., Plonk, S. G., Sutherland, L. L.,... & Xia, S. M. (2008). Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection.Journal of virology, 82(1), 115-125.