Recombinant Human Antibody (PGT127) is capable of binding to HIV Env gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV Env gp120 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV Env gp120 proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 Mutation of select residues interacting with the secondary glycan-binding site that recognizes the N301 glycan.
Mutation of VH H52aA results in a decrease in gp120 binding, whereas disruption of the CDR H1-H2 disulfide (VH C32A or C52bA, or a double mutant) greatly compromises both gp120 binding.
Pejchal, R., Doores, K. J., Walker, L. M., Khayat, R., Huang, P. S., Wang, S. K., ... & Depetris, R. (2011). A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield. Science, 334(6059), 1097-1103.
Figure 2 Neutralization properties of PGT 127 IgGs and Fabs.
Neutralizing activity of PGT 127 IgGs and Fabs against HIV-1JR-FL.
Pejchal, R., Doores, K. J., Walker, L. M., Khayat, R., Huang, P. S., Wang, S. K., ... & Depetris, R. (2011). A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield. Science, 334(6059), 1097-1103.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B053(A) | Recombinant Anti-HIV gp120 Intrabody [(D-Arg)9] | ELISA, ICC, FuncS | scFv-(D-Arg)9 |
IAB-B053(G) | Recombinant Anti-HIV gp120 Intrabody [+36 GFP] | PCA, Neut, FuncS | scFv-(+36GFP) |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-412 | Recombinant Human Anti-HIV gp120 Antibody | WB, Dot, FuncS | IgG |
HPAB-1859-FY | Human Anti-HIV gp120 Recombinant Antibody (clone pMT4-3) | Neut | Human IgG |
FAMAB-0080WJ | Human Anti-HIV gp120 Recombinant Antibody (clone NO.13) | ELISA, WB | Human IgG1, λ |
FAMAB-0081WJ | Human Anti-HIV gp120 Recombinant Antibody (clone S1-1) | ELISA, WB | Human IgG1, λ |
FAMAB-0083WJ | Human Anti-HIV gp120 Recombinant Antibody (clone HBW4-1) | ELISA, WB | Human IgG1, λ |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-412-S(P) | Recombinant Human Anti-HIV gp120 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
HPAB-0556YY-S(P) | Human Anti-HIV gp120 Recombinant Antibody (clone DRVIA7H+gDRVI01-L57); scFv Fragment | ELISA, Neut | Human scFv |
HPAB-0555YY-S(P) | Human Anti-HIV gp120 Recombinant Antibody (clone DRVIA7H+gDRVI01-L40); scFv Fragment | ELISA, Neut | Human scFv |
HPAB-0543YY-S(P) | Human Anti-HIV gp120 Recombinant Antibody; scFv Fragment (HPAB-0543YY-S(P)) | ELISA, FuncS | Human scFv |
PABJ-0031-S(P) | Bovine Anti-HIV gp120 Recombinant Antibody; scFv Fragment (PABJ-0031-S(P)) | ELISA, Neut | Bovine scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-507 | Human Anti-HIV gp120 Recombinant Antibody (clone PGT127); Fab Fragment | ELISA, Neut | Human Fab |
HPAB-1859-FY-S(P) | Human Anti-HIV gp120 Recombinant Antibody (clone pMT4-3); Fab Fragment | Neut | Human Fab |
FAMAB-0080WJ-F(E) | Human Anti-HIV gp120 Recombinant Antibody (clone NO.13); Fab Fragment | ELISA, WB | Human Fab |
FAMAB-0081WJ-F(E) | Human Anti-HIV gp120 Recombinant Antibody (clone S1-1); Fab Fragment | ELISA, WB | Human Fab |
FAMAB-0083WJ-F(E) | Human Anti-HIV gp120 Recombinant Antibody (clone HBW4-1); Fab Fragment | ELISA, WB | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-182LC | Anti-HIV gp120 Recombinant Antibody (58.2) | ELISA | Human antibody |
MRO-183LC | Anti-HIV gp120 Recombinant Antibody (59.1) | ELISA | Human antibody |
MRO-182LC-S(P) | Anti-HIV gp120 Recombinant Antibody scFv Fragment (58.2) | ELISA | Human antibody |
MRO-182LC-F(E) | Anti-HIV gp120 Recombinant Antibody Fab Fragment (58.2) | ELISA | Human antibody |
MRO-183LC-F(E) | Anti-HIV gp120 Recombinant Antibody Fab Fragment (59.1) | ELISA | Human antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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