Anti-Human HSP60 Recombinant Antibody scFv Fragment (prozumab) (CAT#: TAB-110CT-S(P))

Recombinant monoclonal antibody to HSP60. This antibody intended for the prophylaxis and treatment of Rheumatoid arthritis (RA).


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  • Size:
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  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 Dose response of prozumab binding to recombinant mammalian HSP60 in ELISA.

Figure 1 Dose response of prozumab binding to recombinant mammalian HSP60 in ELISA.

Ulmansky, R., Landstein, D., Moallem, E., Loeb, V., Levin, A., Meyuhas, R.,... & Naparstek, Y. (2015). A humanized monoclonal antibody against heat shock protein 60 suppresses murine arthritis and colitis and skews the cytokine balance toward an anti-inflammatory response. The Journal of Immunology, 194(11), 5103-5109.

Figure 2 Prozumab suppresses AA and CIA.

Figure 2 Prozumab suppresses AA and CIA.

(A) AA was induced in Lewis rats. Rats were treated i.p. with prozumab (2.5 mg/kg) or PBS on day 15. MPS (5 mg/kg) was administered s.c. on day 15. Disease severity was evaluated every few days. Arthritis score is the mean ± SE of seven animals. *p < 0.05 for prozumab compared with PBS-treated rats. (B) Representative joint images of formalin-fixed paraffin-embedded sections prepared from rats treated with PBS or prozumab, as described in (A). Arrows indicate inflammation and fibrosis. Scale bars, 1 mm. (C) CIA was induced in DBA/1 mice. Mice were treated with PBS, prozumab (20 mg/kg), rituximab (20 mg/kg), or Enbrel (10 mg/kg). Two injections were given i.p.: on the day of arthritis onset and 4 d later. Paw diameter change was calculated based on the difference between paw diameter on the specified day and the initial paw diameter on the day of arthritis onset, as assessed by caliper. *p < 0.05 for prozumab compared with rituximab. (D) IL-10 levels (mean ± SE) in the sera of eight to nine CIA-induced mice, treated as described in (C) and sacrificed at the end of the study. *p < 0.05 for prozumab compared with all other treatment groups.

Ulmansky, R., Landstein, D., Moallem, E., Loeb, V., Levin, A., Meyuhas, R.,... & Naparstek, Y. (2015). A humanized monoclonal antibody against heat shock protein 60 suppresses murine arthritis and colitis and skews the cytokine balance toward an anti-inflammatory response. The Journal of Immunology, 194(11), 5103-5109.

Figure 3 Prozumab ameliorates colitis in IBD mouse models.

Figure 3 Prozumab ameliorates colitis in IBD mouse models.

(A) Colitis was induced by TNBS. Reduction of body weight was measured in TNBS-induced colitis mice treated with prozumab (0.8 or 8 mg/kg), PBS, or rituximab (8 mg/kg). Treatment was administered i.p. twice, 2 d prior to and 1 d following intrarectal induction with TNBS. Body weight loss was calculated comparing mice weight at sacrifice to their weight at the intrarectal TNBS administration. Results are mean ± SE of eight mice per group. *p < 0.05 for prozumab (8 mg/kg) compared with PBS and rituximab-treated mice. (B) Representative images of formalin-fixed paraffin-embedded colon sections of mice treated with PBS and prozumab (8 mg/kg), as described in (A). Scale bars, 200 μm. (C) Histopathological analysis of the colon tissues taken from the TNBS-induced mice. Colon sections were scored for histopathologic changes. Results are the mean ± SE of seven to eight mice per group. *p < 0.05 for prozumab (8 mg/kg) compared with PBS and rituximab. (D) Disease score of prozumab (8 mg/kg) and control-treated mice in spontaneous colitis, developed in IL-10–deficient mice. Results are mean ± SE of six to seven mice per group. *p < 0.05 for prozumab compared with PBS and rituximab. (E) Mice survival in the various treatment groups of the spontaneous colitis model. *p < 0.05 for prozumab compared with PBS.

Ulmansky, R., Landstein, D., Moallem, E., Loeb, V., Levin, A., Meyuhas, R.,... & Naparstek, Y. (2015). A humanized monoclonal antibody against heat shock protein 60 suppresses murine arthritis and colitis and skews the cytokine balance toward an anti-inflammatory response. The Journal of Immunology, 194(11), 5103-5109.

Figure 4 Prozumab modulates cytokine secretion from human PBMCs.

Figure 4 Prozumab modulates cytokine secretion from human PBMCs.

IFN-γ (A) and IL-6 (B) levels in supernatants from anti–CD3-activated human PBMCs, incubated without (no Ab) or with 200 μg/ml prozumab or an isotype control Ab for 24 h, were determined by ELISA. Results obtained from each of the six naive donors tested are shown. The inset depicts the calculated mean ± SE of relative reduction in cytokine secretion from Ab-treated cells compared with nontreated cells (no Ab) for these six donors. For the calculation, cytokine secretion of nontreated cells (no Ab) for each donor was defined as 1. (C and D) Effect of prozumab dose on cytokine secretion (IFN-γ and IL-6, respectively) from anti–CD3-activated cryopreserved human PBMCs from two naive donors.

Ulmansky, R., Landstein, D., Moallem, E., Loeb, V., Levin, A., Meyuhas, R.,... & Naparstek, Y. (2015). A humanized monoclonal antibody against heat shock protein 60 suppresses murine arthritis and colitis and skews the cytokine balance toward an anti-inflammatory response. The Journal of Immunology, 194(11), 5103-5109.

Figure 5 Prozumab binding to Jurkat cells by FACS analysis.

Figure 5 Prozumab binding to Jurkat cells by FACS analysis.

(A) Jurkat cells (0.7–1 × 10⁶/ml) stained using prozumab and the isotype control mAbs, rituximab or Avastin, at a concentration of 100 μg/ml followed by allophycocyanin-conjugated anti-human Ab or the secondary Ab only. (B) Percentage binding of prozumab (80 μg/ml) or prozumab in the presence of 120 μg/ml soluble human HSP60 to Jurkat cells. Rituximab served as the isotype control. Detection was done using allophycocyanin-conjugated anti-human Ab.

Ulmansky, R., Landstein, D., Moallem, E., Loeb, V., Levin, A., Meyuhas, R.,... & Naparstek, Y. (2015). A humanized monoclonal antibody against heat shock protein 60 suppresses murine arthritis and colitis and skews the cytokine balance toward an anti-inflammatory response. The Journal of Immunology, 194(11), 5103-5109.


Specifications

  • Host Species
  • Human
  • Derivation
  • Hybridoma cell line
  • Type
  • Humanized antibody
  • Specificity
  • Human
  • Species Reactivity
  • Human
  • Clone
  • prozumab
  • Applications
  • ELISA, Inhib, FuncS
  • Related Disease
  • Rheumatoid arthritis (RA)

Applications

  • Application Notes
  • The antibody was validated for ELISA, Inhibition and Function assay. For details, refer to Published Data.

Target

  • Alternative Names
  • HSPD1; heat shock 60kDa protein 1 (chaperonin); HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; 60 kDa heat shock protein; mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1; anti-HSP65

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Humanized Antibody

CAT Product Name Application Type
TAB-110CT Anti-Human HSP60 Recombinant Antibody (prozumab) Inhibit, ELISA Humanized antibody
TAB-110CT-F(E) Anti-Human HSP60 Recombinant Antibody Fab Fragment (prozumab) Inhibit, ELISA Humanized antibody

Mouse Antibody

CAT Product Name Application Type
MRO-018CT Recombinant Mouse Anti-HSP60 Antibody WB, IHC, FC

Blocking Antibody

CAT Product Name Application Type
NEUT-920CQ Mouse Anti-HSPD1 Recombinant Antibody (clone 4B9/89) Block, ELISA, ICC, IF, IHC, IHC-P, IP, WB Mouse IgG2a

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1704 Rabbit Anti-HSPD1 Recombinant Antibody (clone DS1704AB) IHC-P, WB Rabbit IgG
MOR-0077-FY Rabbit Anti-HSPD1 Recombinant Antibody (clone AFY0048) ICC, IHC-P, WB Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC2077 PE-A*02:01/Human HSPD1 (KLSDGVAVL) MHC Tetramer FCM

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-1428LC Mouse Anti-HSPD1 Recombinant Antibody (clone 9C9) ELISA Mouse IgG1

MHC Tetramer for Other Applications

CAT Product Name Application Type
MHC-YF309 Qa-1b/Human Hsp60 (GMKFDRGYI) MHC Monomer MHC Multimer

scFv Fragment Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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