Anti-HTNV G1 Recombinant Antibody (8B6) (CAT#: MRO-1070LC)

Figure 1 Intracellular and cell surface expression of G1 and G2 glycoproteins in 293T cells.

Intracellular staining. Transfected 293T cells were resuspended in PBS, spotted onto a 24-well glass slide, and air dried. After being fixed with acetone, the cells were treated with the culture supernatant from hybridoma clones producing the MAb 8B6 for G1 and MAb HCO2 for G2

Use of Vesicular Stomatitis Virus Pseudotypes Bearing Hantaan or Seoul Virus Envelope Proteins in a Rapid and Safe Neutralization Test

Figure 2 Expression from pWRG/AND-M.

The indicated HTNV-specific MAbs, HTNV-specific mouse hyperimmune ascitic fluid (HTN-poly), or HPS convalescent-phase serum from Argentina (AND-poly) was used to immunoprecipitate proteins from COS cells transfected with pWRG/HTN-M(x) (H) or pWRG/AND-M (A). +, G1 or G2 was immunoprecipitated; −, no protein was immunoprecipitated; *, both G1 and G2 were immunoprecipitated.

Active and Passive Vaccination against Hantavirus Pulmonary Syndrome with Andes Virus M Genome Segment-Based DNA Vaccine

Figure 3 Incorporation of G1 and G2 glycoproteins into pseudotype VSVΔG*.

Detection of hantaviral G1 and G2 proteins by MAbs in pseudotype VSVΔG* after native 8% PAGE separation. Lanes: 1, VSV ΔG*G; 2, VSVΔ G*-HTN; 3, VSVΔG*-SEO.

Use of Vesicular Stomatitis Virus Pseudotypes Bearing Hantaan or Seoul Virus Envelope Proteins in a Rapid and Safe Neutralization Test