Anti-HTNV G2 Recombinant Antibody (16E6) (CAT#: MRO-1076LC)

Figure 1 Expression from pWRG/AND-M.

The indicated HTNV-specific MAbs, HTNV-specific mouse hyperimmune ascitic fluid (HTN-poly), or HPS convalescent-phase serum from Argentina (AND-poly) was used to immunoprecipitate proteins from COS cells transfected with pWRG/HTN-M(x) (H) or pWRG/AND-M (A). +, G1 or G2 was immunoprecipitated; −, no protein was immunoprecipitated; *, both G1 and G2 were immunoprecipitated. Molecular mass marker (M) sizes in kilodaltons are shown on the left, and the positions of G1, G2, and N are shown on the right.

Active and Passive Vaccination against Hantavirus Pulmonary Syndrome with Andes Virus M Genome Segment-Based DNA Vaccine

Figure 2 Polyacrylamide gel electrophoresis of Hantaan virus proteins.

The specificities of secreted MAbs were determined by immune precipitation of viral proteins radiolabelled with L-[3SS]methionine. The Mr values of L-t4C-methylated protein markers (lane 1) are listed on the left. Arrows indicate Hantaan viral envelope (G1 and G2) and nucleocapsid (NC) proteins which were precipitated with polyclonal, hyperimmune mouse ascitic fluid (lane 2). Precipitation by Gl-specific MAbs representing each of the two com- petition groups (1 OF 11, lane 3; 16D2, lane 4), or G2-specific MAbs representing three of five competition groups (5B7, lane 6; 1C6, lane 7; 16E6, lane 8) are shown. Although MAb 2D5 appeared to precipitate both G 1 and G2 (lane 5), competition analysis revealed it to be Gl-specific.

Characterization of Hantaan Virus Envelope Glycoprotein Antigenic Determinants Defined by Monoclonal Antibodies