Recombinant Human Antibody (C4) is capable of binding to HTT, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HTT mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HTT proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody inhibits amyloid formation by exon1 fragments of huntingtin in vitro.
Figure 1 Direct binding of C4 sFv to immobilized GST-fusion proteins analyzed by ELISA.
C4 sFv-myc-His6 antibodies were quantitated in microtiter plates coated with 70 nM of GST-fusion proteins and blocked with 1% BSA in PBS. Bound sFv were detected with anti-myc antibodies (9E10, 1 μg/ml), followed by alkaline phosphatase-conjugated goat anti-mouse IgG.
Lecerf, J. M., Shirley, T. L., Zhu, Q., Kazantsev, A., Amersdorfer, P., Housman, D. E., ... & Huston, J. S. (2001). Human single-chain Fv intrabodies counteract in situ huntingtin aggregation in cellular models of Huntington's disease. Proceedings of the National Academy of Sciences, 98(8), 4764-4769.
Figure 2 Immunofluorescent analysis of GFP-fusion HD fragments to the nucleus by anti-HD C4 sFv-HA-nls.
Immunofluorescence of HD-Q103-myc-His6 with C4 sFv-HA-nls (b, d, f) or a negative control sFv-HA-nls intrabody (a, c, e), after 48-h cotransfection in COS-7. HD-Q103-myc-His6 was detected by 9E10 mAb, followed by FITC-labeled goat anti-mouse IgG antibodies (c, d). The intrabodies were detected with polyclonal anti-HA antibodies, followed by rhodamine-labeled goat anti-rabbit IgG antibodies (a, b). (e, f) Dual staining of the same fields.
Lecerf, J. M., Shirley, T. L., Zhu, Q., Kazantsev, A., Amersdorfer, P., Housman, D. E., ... & Huston, J. S. (2001). Human single-chain Fv intrabodies counteract in situ huntingtin aggregation in cellular models of Huntington's disease. Proceedings of the National Academy of Sciences, 98(8), 4764-4769.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
NAB-1905-VHH | Recombinant Anti-human HTT VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
PABJ-0201 | Human Anti-HTT Recombinant Antibody (clone 3LRH) | ELISA, FuncS | Human VL |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-521 | Recombinant Anti-human HTT Antibody | WB, Neut, FuncS | IgG |
PABL-581 | Human Anti-HTT Recombinant Antibody (clone E51) | FC, ELISA | Human IgG |
HPAB-0122-YC | Mouse Anti-HTT Recombinant Antibody (HPAB-0122-YC) | FuncS, WB, Inhib, IHC | Mouse IgM |
HPAB-0123-YC | Mouse Anti-HTT Recombinant Antibody (HPAB-0123-YC) | IHC, WB, Inhib | Mouse IgG2a |
HPAB-0125-YC | Human Anti-HTT Recombinant Antibody (HPAB-0125-YC) | ELISA, IHC | Humanized IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-521-S(P) | Recombinant Anti-human HTT Antibody scFv Fragment | FC, Neut, Funcs | scFv |
MHH-521-S(P) | Recombinant Human Anti-human HTT Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
PSBL-575 | Human Anti-HTT Recombinant Antibody (clone C4); scFv Fragment | Block | Human scFv |
PSBL-576 | Human Anti-HTT Recombinant Antibody (clone E51); scFv Fragment | FC, ELISA | Human scFv |
HPAB-0121-YC-S(P) | Mouse Anti-HTT Recombinant Antibody (clone MW1); scFv Fragment | ELISA, FC, WB, IHC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B023(A) | Recombinant Anti-human HTT Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
IAB-B023(G) | Recombinant Anti-human HTT Intrabody [+36 GFP] | IF, FC, WB, FuncS | scFv-(+36GFP) |
IAB-B023(T) | Recombinant Anti-human HTT Intrabody [Tat] | WB, CO-IP, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-115CT | Mouse Anti-HTT Recombinant Antibody (TAB-115CT) | ELISA, WB | Mouse IgG2a |
TAB-116CT | Mouse Anti-HTT Recombinant Antibody (TAB-116CT) | ELISA, WB | Mouse IgG2a |
TAB-117CT | Mouse Anti-HTT Recombinant Antibody (TAB-117CT) | ELISA, WB | Mouse IgG2a |
PABX-108 | Recombinant Mouse Anti-Huntingtin (Htt) Antibody (3B5H10) | WB, ELISA, FuncS | IgG |
PABX-109-S (P) | Recombinant Human Anti-Huntingtin (Htt) Antibody scFv Fragment | WB, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-114CT-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (TAB-114CT-S(P)) | ELISA, WB | Human scFv |
TAB-114CT-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (TAB-114CT-F(E)) | ELISA, WB | Human Fab |
PABX-109 | Recombinant Human Anti-Huntingtin (Htt) Antibody | WB, Neut, FuncS | IgG |
PABX-108-S (P) | Recombinant Mouse Anti-Huntingtin (Htt) Antibody scFv Fragment (3B5H10) | WB, ELISA, FuncS | scFv |
PABX-109-F (E) | Recombinant Human Anti-Huntingtin(Htt) Antibody Fab Fragment | WB, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1714 | Rabbit Anti-HTT Recombinant Antibody (clone DS1714AB) | WB, IHC-P, ICC | Rabbit IgG |
MOR-0066-FY | Rabbit Anti-HTT Recombinant Antibody (clone AFY0037) | FC, ICC, IHC, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0121-YC-F(E) | Mouse Anti-HTT Recombinant Antibody (clone MW1); Fab Fragment | ELISA, FC, WB, IHC | Mouse Fab |
HPAB-0122-YC-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0122-YC-F(E)) | ELISA, FC, WB, IHC | Mouse Fab |
HPAB-0123-YC-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0123-YC-F(E)) | ELISA, FC, WB, IHC | Mouse Fab |
HPAB-0125-YC-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0125-YC-F(E)) | ELISA, IHC | Humanized Fab |
HPAB-0126-YC-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0126-YC-F(E)) | ELISA, IHC | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-039CN | Human Anti-HTT Recombinant Antibody (NS-039CN) | ELISA, DB, IHC, FuncS | Human IgG |
NS-039CN-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (NS-039CN-F(E)) | ELISA | Human Fab |
NS-039CN-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (NS-039CN-S(P)) | ELISA | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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