Recombinant Anti-human HTT Intrabody [(D-Arg)9] (CAT#: IAB-B023(A))
Recombinant anti-HTT intrabody linked to (D-Arg)9 works within cells and directs against htt and prevent aggregation, show promise as immunotherapeutics for HD, expressed in 293T cells. The anti-huntingtin intrabody can degrade mutant huntingtin proteins by directing them to the proteasome, while also increasing the negative charge.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg
Figure 6 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg
Figure 7 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg
Figure 9 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg
Specifications
- Immunogen
- human Huntingtin
- Host Species
- human
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Products can be used for: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies
Product Property
- Purity
- >90% assessed by SDS-PAGE
- Storage
- Store at -20°C. Avoid multiple freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HTT"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1905-VHH | Recombinant Anti-human HTT VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| PABJ-0201 | Human Anti-HTT Recombinant Antibody (clone 3LRH) | ELISA, FuncS | Human VL |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-521-S(P) | Recombinant Anti-human HTT Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| MHH-521-S(P) | Recombinant Human Anti-human HTT Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| PSBL-575 | Human Anti-HTT Recombinant Antibody (clone C4); scFv Fragment | Block | Human scFv |
| PSBL-576 | Human Anti-HTT Recombinant Antibody (clone E51); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-0121-YC-S(P) | Mouse Anti-HTT Recombinant Antibody (clone MW1); scFv Fragment | ELISA, FC, WB, IHC | Mouse scFv |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B023(G) | Recombinant Anti-human HTT Intrabody [+36 GFP] | IF, FC, WB, FuncS | scFv-(+36GFP) |
| IAB-B023(T) | Recombinant Anti-human HTT Intrabody [Tat] | WB, CO-IP, FuncS | scFv-Tat |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-521 | Recombinant Human Anti-human HTT Antibody | WB, Neut, FuncS | IgG |
| PABL-580 | Human Anti-HTT Recombinant Antibody (clone C4) | Block | Human IgG |
| PABL-581 | Human Anti-HTT Recombinant Antibody (clone E51) | FC, ELISA | Human IgG |
| MOB-0905CT | Recombinant Mouse anti-Human HTT Monoclonal antibody (3502D2b) | Dot, WB | |
| HPAB-0122-YC | Mouse Anti-HTT Recombinant Antibody (HPAB-0122-YC) | FuncS, WB, Inhib, IHC | Mouse IgM |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-521-F(E) | Recombinant Human Anti-human HTT Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| PFBL-575 | Human Anti-HTT Recombinant Antibody (clone C4); Fab Fragment | Block | Human Fab |
| PFBL-576 | Human Anti-HTT Recombinant Antibody (clone E51); Fab Fragment | FC, ELISA | Human Fab |
| HPAB-0121-YC-F(E) | Mouse Anti-HTT Recombinant Antibody (clone MW1); Fab Fragment | ELISA, FC, WB, IHC | Mouse Fab |
| HPAB-0122-YC-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0122-YC-F(E)) | ELISA, FC, WB, IHC | Mouse Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-114CT | Human Anti-HTT Recombinant Antibody (TAB-114CT) | ELISA, WB | Human IgG1, κ |
| TAB-114CT-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (TAB-114CT-S(P)) | ELISA, WB | Human scFv |
| TAB-114CT-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (TAB-114CT-F(E)) | ELISA, WB | Human Fab |
| PABX-109 | Recombinant Human Anti-Huntingtin (Htt) Antibody | WB, Neut, FuncS | IgG |
| PABX-109-F (E) | Recombinant Human Anti-Huntingtin(Htt) Antibody Fab Fragment | WB, Neut, FuncS | Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-115CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-115CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-116CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-116CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-117CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-117CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-115CT-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (TAB-115CT-F(E)) | ELISA, WB | Mouse Fab |
| PABX-109-S (P) | Recombinant Human Anti-Huntingtin (Htt) Antibody scFv Fragment | WB, Neut, FuncS | scFv |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1714 | Rabbit Anti-HTT Recombinant Antibody (clone DS1714AB) | WB, IHC-P, ICC | Rabbit IgG |
| MOR-0066-FY | Rabbit Anti-HTT Recombinant Antibody (clone AFY0037) | FC, ICC, IHC, WB | Rabbit IgG |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-039CN | Human Anti-HTT Recombinant Antibody (NS-039CN) | ELISA, DB, IHC, FuncS | Human IgG |
| NS-039CN-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (NS-039CN-F(E)) | ELISA | Human Fab |
| NS-039CN-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (NS-039CN-S(P)) | ELISA | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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