Mouse Anti-ABCB1 Recombinant Antibody (clone C219) (CAT#: PABC-052)
This antibody is a mouse monoclonal antibody C219 that binds specifically to ABCB1, and it can neutralize the bioactivity of ABCB1.
Specific Inquiry
IHC
Figure 1 Photomicrograph of untreated lymphoma in a dog utilizing C219 MAb immunohistochemistry technique.
Lack of red staining areas denotes lack of C219 staining (original magnification x400).
Bergman P J , Ogilvie G K , Powers B E. Monoclonal Antibody C219 Immunohistochemistry Against P-Glycoprotein: Sequential Analysis and Predictive Ability in Dogs With Lymphoma[J]. Journal of Veterinary Internal Medicine, 1996, 10(6):354-359.
IHC
Figure 2 Photomicrograph of relapsed lymphoma in a dog utilizing C219 MAb immunohistochemistry technique.
Note that red staining areas show C219 staining (original magnification x400).
Bergman P J , Ogilvie G K , Powers B E. Monoclonal Antibody C219 Immunohistochemistry Against P-Glycoprotein: Sequential Analysis and Predictive Ability in Dogs With Lymphoma[J]. Journal of Veterinary Internal Medicine, 1996, 10(6):354-359.
WB
Figure 3 Western blot analysis.
A: Western blot analysis of cell membrane proteins of BC19/3 (lane 1,25 ug), MCF-7 (lane 2, 200 ug), BALB/c-3T3 (lane 3, 200 ug), and KB-Vl cells (lane 4, 10 ug) with C219 antibody.
Han, E. S., Vanoye, C. G., Altenberg, G. A., & Reuss, L. U. I. S. (1996). P-glycoprotein-associated chloride currents revealed by specific block by an anti-P-glycoprotein antibody. American Journal of Physiology-Cell Physiology, 270(5), C1370-C1378.
IF
Figure 4 Immunofluorescence studies.
Immunofluorescence studies of PGP expression with fluorescem isothiocyanate-labeled C219 in MCF-7 and BC19/3 cells. Bars denote 10 um.
Han, E. S., Vanoye, C. G., Altenberg, G. A., & Reuss, L. U. I. S. (1996). P-glycoprotein-associated chloride currents revealed by specific block by an anti-P-glycoprotein antibody. American Journal of Physiology-Cell Physiology, 270(5), C1370-C1378.
WB
Figure 5 Western blot using C219 antibody.
(a) 50 μg of mouse brain capillary extract; (b) 25 μg proteins from MBCECs cocultivated with glial cells; (c) 50 μg proteins from mouse glial cells.
Coisne, C., Dehouck, L., Faveeuw, C., Delplace, Y., Miller, F., Landry, C., ... & Dehouck, B. (2005). Mouse syngenic in vitro blood–brain barrier model: a new tool to examine inflammatory events in cerebral endothelium. Laboratory investigation, 85(6), 734.
Specifications
- Immunogen
- Hamster ATP binding cassette subfamily B member 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Hamster Abcb1
- Species Reactivity
- Human, Mouse, Rat, Zebrafish, Bovine, Hamster
- Clone
- C219
- Applications
- WB, IF, IHC, FC, FuncS
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody C219 has been reported in applications of Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone C219"
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-F(E) | Recombinant Anti-human ABCB1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-22-F(E) | Recombinant Human Anti-human ABCB1 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); Fab Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse IgG |
| PFBC-444 | Human Anti-ABCB1 Recombinant Antibody (clone UIC2); Fab Fragment | FC, Block, IP, IHC | Human Fab |
| HPAB-M0001-YC-F(E) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); Fab Fragment | IHC | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-S(P) | Recombinant Anti-human ABCB1 Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| MHH-22-S(P) | Recombinant Human Anti-human ABCB1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| PSBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); scFv Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse scFv |
| PSBC-444 | Human Anti-ABCB1 Recombinant Antibody (clone UIC2); scFv Fragment | FC, Block, IP, IHC | Mouse scFv |
| HPAB-M0001-YC-S(P) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); scFv Fragment | IHC | Mouse scFv |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-001CQ | Mouse Anti-ABCB1 Recombinant Antibody (clone UIC2) | FC, Block, IP, IHC | Mouse IgG2a, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0009 | Hi-Affi™ Rabbit Anti-ABCB1 Recombinant Antibody (clone DS9AB) | WB, IHC-P | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1138-CN | Rabbit Anti-ABCB1 Recombinant Antibody (clone CBACN-413) | WB, IHC | Rabbit IgG |
| HPAB-0076-YJ | Mouse Anti-ABCB1 Recombinant Antibody (clone MM4.17) | IF, FC, IHC-P, ELISA | Mouse IgG2a, κ |
| VS3-XY2 | Mouse Anti-ABCB1 Recombinant Antibody (clone 2G1) | WB, IP, IF, IHC | Mouse IgG2a |
| VS3-XY3 | Mouse Anti-ABCB1 Recombinant Antibody (clone 6G11C12) | ELISA, WB | Mouse IgG1 |
| VS3-FY5 | Recombinant Rabbit Anti-ABCB1 Antibody (clone R02-6B2) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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