Anti-Hepatitis C virus antibody is a Human of scFv class that binds to an Hepatitis C virus.
Figure 1 Analysis of E1E2 of diverse HCV genotypes.
Radiolabeled proteins in the lysate of HEK 293T cells cotransfected with different plasmids as described in A above were immunoprecipitated using a mixture of anti-E2 MAbs AP33 and ALP98. The immune complexes were analyzed by SDS-10% PAGE under reducing conditions. Positions of protein size markers are shown (in kDa).
Owsianka, A., Tarr, A. W., Juttla, V. S., Lavillette, D., Bartosch, B., Cosset, F. L., ... & Patel, A. H. (2005). Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoprotein. Journal of virology, 79(17), 11095-11104.
Figure 2 Lysine residues upstream of the conserved epitope 412-423 are important for mAb AP33 binding.
The binding pattern of mAb 3/11 and AP33 was compared using wild type or mutated patientderived peptides spanning AA region 406-427. Serial dilutions of mAbs were incubated with surface-attached peptides (25 lg/mL), and bound antibodies were detected with a horseradish peroxidase–conjugated antispecies secondary antibody. Peptides were generated with K- or A-substitutions at positions 408 and/or 410 (H77c: K408A, N410K, N410A, and double mutants K408A-N410K and K408A-N410A; LT-P05_VF: T408A, T408K, K410A, and double mutants T408K-K410A and T408A-K410A; CC-P08_VA: A408K, K410A, and double mutant A408KK410A). The resulting peptide sequences are shown and changed AA marked. Dots indicate AA similarity.
Desombere, I., Fafi‐Kremer, S., Van Houtte, F., Pessaux, P., Farhoudi, A., Heydmann, L., ... & Baumert, T. F. (2016). Monoclonal anti‐envelope antibody AP33 protects humanized mice against a patient‐derived hepatitis C virus challenge. Hepatology, 63(4), 1120-1134.
Figure 3 mAb AP33 protects humanized mice against an HCV gt1b challenge.
Chimeric animals were challenged with 104 IU of HCV RNA of mLT_P05 without pretreatment (no IgG) or after passive immunization on day 23 with 1 mg of mAb 3/11 or AP33. Plasma HCV-RNA levels (IU/mL) were measured until 8 weeks after viral challenge. Mice that remained noninfected postinoculation are shown as HCV RNA <750 IU/mL (LOQ, dotted line). Animal B338RL died 4 weeks after viral challenge.
Desombere, I., Fafi‐Kremer, S., Van Houtte, F., Pessaux, P., Farhoudi, A., Heydmann, L., ... & Baumert, T. F. (2016). Monoclonal anti‐envelope antibody AP33 protects humanized mice against a patient‐derived hepatitis C virus challenge. Hepatology, 63(4), 1120-1134.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-444-S(P) | Recombinant Anti-HCV Antibody scFv Fragment | WB, IHC, FuncS | scFv |
PSBZ-057 | Recombinant Mouse Anti-HCV Antibody scFv Fragment (19D9D6) | ELISA | scFv |
HPAB-1118LY-S(P) | Humanized Anti-HCV Antibody scFv Fragment (CBL021) | ELISA, FC | |
HPAB-1119LY-S(P) | Humanized Anti-HCV Antibody scFv Fragment (CBL022) | ELISA, FC | |
HPAB-N0132-YC-S(P) | Recombinant Human Anti-HCV Antibody scFv Fragment (TRN-1002) | ELISA, Neut | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-079 | Recombinant Anti-HCV VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-444 | Recombinant Human Anti-HCV Antibody | IF, Neut, FuncS | IgG |
HPAB-0043LY | Recombinant Mouse Anti-HCV Antibody (PA-29) | WB | |
HPAB-0232-FY | Recombinant Human Anti-HCV Antibody (TRN1007) | WB, F, N | Human IgG |
HPAB-0233-FY | Recombinant Human Anti-HCV Antibody (TRN1009) | WB, E, N | Human IgG |
FAMAB-0024WJ | Human Anti-HCV Recombinant Antibody (clone B12.F8) | ELISA, IF, IHC, WB | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-344CL | Human Anti-HCV Recombinant Antibody (TAB-344CL) | FuncS | Human IgG |
PABX-071 | Recombinant Human Anti-HCV Antibody (HC33.4) | WB, ELISA, Neut, FuncS | IgG |
PABX-079 | Recombinant Human Anti-Hepatitis C virus Antibody (AP33) | WB, ELISA, FuncS | IgG |
PABX-071-F (E) | Recombinant Human Anti-HCV Antibody Fab Fragment (HC33.4) | WB, ELISA, Neut, FuncS | Fab |
PABX-079-F (E) | Recombinant Human Anti-Hepatitis C virus Antibody Fab Fragment (AP33) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-071-S (P) | Recombinant Human Anti-HCV Antibody scFv Fragment (HC33.4) | WB, ELISA, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1118LY-F(E) | Humanized Anti-HCV Antibody Fab Fragment (CBL021) | ELISA, FC | |
HPAB-1119LY-F(E) | Humanized Anti-HCV Antibody Fab Fragment (CBL022) | ELISA, FC | |
HPAB-1120LY-F(E) | Humanized Anti-HCV Antibody Fab Fragment (CBL023) | ELISA, FC | |
HPAB-1121LY-F(E) | Humanized Anti-HCV Antibody Fab Fragment (CBL024) | ELISA, FC | |
HPAB-0232-FY-F(E) | Recombinant Human Anti-HCV Antibody Fab Fragment (TRN1007) | WB, F, N | Human Fab |
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