Anti-Human CD40L Recombinant Antibody (Ruplizumab) (CAT#: TAB-027)

Recombinant monoclonal antibody to Human CD40L. Ruplizumab is a humanized monoclonal antibody intended for the treatment of rheumatic diseases like systemic lupus erythematosus and lupus nephritis. A study showed that the drug was associated with life-threatening thromboembolisms, while another study only found thrombocytopenia.


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SDS-PAGE

Figure 1 Anti-Human CD40L Recombinant Antibody (TAB-027) in SDS-PAGE

Figure 1 Anti-Human CD40L Recombinant Antibody (TAB-027) in SDS-PAGE

SDS-PAGE analysis of TAB-027 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180-275 kDa.

SEC-HPLC

Figure 2 Anti-Human CD40L Recombinant Antibody (TAB-027) in SEC-HPLC

Figure 2 Anti-Human CD40L Recombinant Antibody (TAB-027) in SEC-HPLC

The purity of TAB-027 was greater than 99% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

DB

Figure 3 Anti-Human CD40L Recombinant Antibody (TAB-027) in DB

Figure 3 Anti-Human CD40L Recombinant Antibody (TAB-027) in DB

Dot blot analysis of TAB-027 was performed by loading Recombinant Human CD40L (His Tag).

TAB-027 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

WB

Figure 4 Anti-Human CD40L Recombinant Antibody (TAB-027) in WB

Figure 4 Anti-Human CD40L Recombinant Antibody (TAB-027) in WB

Western blot analysis of TAB-027 was performed by loading Recombinant Human CD40L (His Tag).

TAB-027 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

ELISA

Figure 5 Anti-Human CD40L Recombinant Antibody (TAB-027) in ELISA

Figure 5 Anti-Human CD40L Recombinant Antibody (TAB-027) in ELISA

ELISA analysis of TAB-027 was performed by coating with Recombinant Human CD40L.
The secondary antibody: HRP-Anti-Human IgG (H+L)


Specifications

  • Immunogen
  • Animals were immunized with D1.1 cells.
  • Host Species
  • Mouse
  • Derivation
  • Humanized (from mouse)
  • Type
  • IgG1 - kappa
  • Specificity
  • Tested positive against native human antigen
  • Species Reactivity
  • Human
  • Applications
  • Suitable for use in IF, IP, Neut, FuncS, ELISA, FC, ICC and most other immunological methods.
  • CAS
  • 220651-94-5
  • Generic Name
  • Ruplizumab
  • Related Disease
  • Allograft rejection

Product Property

  • Purity
  • >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
  • Storage
  • Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.

Target

  • Alternative Names
  • Ruplizumab;220651-94-5;BG9588;hu5c8;CD40LG;CD40 ligand;HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome);CD40 antigen ligand;CD40L;CD154;gp39;hCD40L;hyper IgM syndrome;T B cell activati

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Ruplizumab"

Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Ruplizumab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human CD40L. Ruplizumab is a humanized monoclonal antibody intended for the treatment of rheumatic diseases like systemic lupus erythematosus and lupus nephritis.

See other products for "CD40LG"

Immunotoxin

CAT Product Name Application Type
AGTO-G080E Anti-Cd40lg immunotoxin LL2 (IgG)-PE Cytotoxicity assay, Function study
AGTO-G081E Anti-Cd40lg immunotoxin LL2 (Fab)-PE Cytotoxicity assay, Function study
AGTO-L051P anti-CD40LG immunotoxin MR1 (scFv)-PAP Cytotoxicity assay, Functional assay

Chimeric Antibody

CAT Product Name Application Type
TAB-225LC Anti-Human CD40LG Recombinant Antibody (YMF323.6) ELISA Chimeric antibody (hamster/human)
TAB-225LC-S(P) Anti-Human CD40LG Recombinant Antibody scFv Fragment (YMF323.6) ELISA Chimeric antibody (hamster/human)
TAB-225LC-F(E) Anti-Human CD40LG Recombinant Antibody Fab Fragment (YMF323.6) ELISA Chimeric antibody (hamster/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-368CQ Mouse Anti-CD40LG Recombinant Antibody (clone 24-31) FC, Block, IF Mouse IgG1, κ
NEUT-375CQ Hamster Anti-CD40LG Recombinant Antibody (clone MR1) IHC-Fr, FC, IP, Block Hamster IgG1, κ
NEUT-377CQ Hamster Anti-CD40LG Recombinant Antibody (clone MAB0820) Block, FC, IHC-Fr Hamster IgG

Neutralizing Antibody

Rabbit Monoclonal Antibody

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-0610LC Mouse Anti-CD40LG Recombinant Antibody (clone h5c8) ELISA Mouse IgG1

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-027 Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Ruplizumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-876 Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Toralizumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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