Recombinant monoclonal antibody to Human CD40L. Ruplizumab is a humanized monoclonal antibody intended for the treatment of rheumatic diseases like systemic lupus erythematosus and lupus nephritis. A study showed that the drug was associated with life-threatening thromboembolisms, while another study only found thrombocytopenia.
Figure 1 Anti-Human CD40L Recombinant Antibody (TAB-027) in SDS-PAGE
SDS-PAGE analysis of TAB-027 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180-275 kDa.
Figure 2 Anti-Human CD40L Recombinant Antibody (TAB-027) in SEC-HPLC
The purity of TAB-027 was greater than 99% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Anti-Human CD40L Recombinant Antibody (TAB-027) in DB
Dot blot analysis of TAB-027 was performed by loading Recombinant Human CD40L (His Tag).
TAB-027 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 4 Anti-Human CD40L Recombinant Antibody (TAB-027) in WB
Western blot analysis of TAB-027 was performed by loading Recombinant Human CD40L (His Tag).
TAB-027 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
Figure 5 Anti-Human CD40L Recombinant Antibody (TAB-027) in ELISA
ELISA analysis of TAB-027 was performed by coating with Recombinant Human CD40L.
The secondary antibody: HRP-Anti-Human IgG (H+L)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Ruplizumab), ADCC EnhancedThis product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human CD40L. Ruplizumab is a humanized monoclonal antibody intended for the treatment of rheumatic diseases like systemic lupus erythematosus and lupus nephritis.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-876 | Anti-Human CD40 ligand Recombinant Antibody (Toralizumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
TAB-211LC | Human Anti-CD40LG Recombinant Antibody (TAB-211LC) | ELISA | Human IgG, κ |
TAB-210LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-210LC-S(P)) | ELISA | Humanized scFv |
TAB-211LC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (TAB-211LC-S(P)) | ELISA | Human scFv |
TAB-210LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-210LC-F(E)) | ELISA | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G080E | Anti-Cd40lg immunotoxin LL2 (IgG)-PE | Cytotoxicity assay, Function study | |
AGTO-G081E | Anti-Cd40lg immunotoxin LL2 (Fab)-PE | Cytotoxicity assay, Function study | |
AGTO-L051P | anti-CD40LG immunotoxin MR1 (scFv)-PAP | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBW-020 | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (PFBW-020) | Neut | Human Fab |
HPAB-0179-YC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0179-YC-F(E)) | ELISA | Human Fab |
FAMAB-0135-YC-F(E) | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1); Fab Fragment | ELISA | Hamster Fab |
HPAB-0744LY-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0744LY-F(E)) | ELISA | Human Fab |
HPAB-0880LY-F(E) | Mouse Anti-CD40LG Recombinant Antibody; Fab Fragment (HPAB-0880LY-F(E)) | FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBW-020 | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (PSBW-020) | Neut | Human scFv |
HPAB-0179-YC-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0179-YC-S(P)) | ELISA | Human scFv |
FAMAB-0135-YC-S(P) | Hamster Anti-CD40LG Recombinant Antibody (clone mr-1); scFv Fragment | ELISA | Hamster scFv |
HPAB-0744LY-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0744LY-S(P)) | ELISA | Human scfv |
HPAB-0585-WJ-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (HPAB-0585-WJ-S(P)) | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-212LC | Mouse Anti-Human CD40LG Recombinant Antibody (ABI793) | Block | |
TAB-212LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (ABI793) | ELISA | |
TAB-212LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (ABI793) | ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-225LC | Anti-Human CD40LG Recombinant Antibody (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
TAB-225LC-S(P) | Anti-Human CD40LG Recombinant Antibody scFv Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
TAB-225LC-F(E) | Anti-Human CD40LG Recombinant Antibody Fab Fragment (YMF323.6) | ELISA | Chimeric antibody (hamster/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-213LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-213LC-F(E)) | FC, ELISA, RBA | |
TAB-214LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-214LC-F(E)) | FC, ELISA, RBA | |
TAB-215LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-215LC-F(E)) | FC, ELISA, RBA | |
TAB-216LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-216LC-F(E)) | FC, ELISA, RBA | |
TAB-217LC-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (TAB-217LC-F(E)) | FC, ELISA, RBA |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-368CQ | Mouse Anti-CD40LG Recombinant Antibody (clone 24-31) | FC, Block, IF | Mouse IgG1, κ |
NEUT-375CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MR1) | IHC-Fr, FC, IP, Block | Hamster IgG1, κ |
NEUT-377CQ | Hamster Anti-CD40LG Recombinant Antibody (clone MAB0820) | Block, FC, IHC-Fr | Hamster IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-369CQ | Mouse Anti-CD40LG Recombinant Antibody (clone CBL300) | FC, IHC, CyTOF, Neut, WB | Mouse IgG2b |
NEUT-370CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL2) | Neut, ELISA, FC, FuncS, IF | Mouse IgG1 |
NEUT-371CQ | Mouse Anti-CD40LG Recombinant Antibody (clone LL48) | Neut, FuncS, IF | Mouse IgG2a |
NEUT-372CQ | Mouse Anti-CD40LG Recombinant Antibody (clone 13H5) | Neut | Mouse IgG2b |
NEUT-374CQ | Rat Anti-CD40LG Recombinant Antibody (clone CBL719) | FC, CyTOF, Neut | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0561 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS561AB) | WB, FC | Rabbit IgG |
MOR-0562 | Hi-Affi™ Rabbit Anti-CD40LG Recombinant Antibody (clone DS562AB) | ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0610LC | Mouse Anti-CD40LG Recombinant Antibody (clone h5c8) | ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-022CN | Human Anti-CD40LG Recombinant Antibody (NS-022CN) | ELISA, FC | Human IgG |
NS-022CN-F(E) | Human Anti-CD40LG Recombinant Antibody; Fab Fragment (NS-022CN-F(E)) | ELISA, FC | Human Fab |
NS-022CN-S(P) | Human Anti-CD40LG Recombinant Antibody; scFv Fragment (NS-022CN-S(P)) | ELISA, FC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0744LY | Human Anti-CD40LG Recombinant Antibody (HPAB-0744LY) | Inhib | Human IgG |
MRO-0304-CN | Rabbit Anti-CD40LG Recombinant Antibody (clone JM11-34) | WB, IF, IHC, FC | Rabbit IgG |
HPAB-0585-WJ | Human Anti-CD40LG Recombinant Antibody (HPAB-0585-WJ) | ELISA | Human IgG |
VS3-CJ861 | Mouse Anti-CD40LG Recombinant Antibody (clone M91) | IF, FC | Mouse IgG1 |
VS3-XY339 | Mouse Anti-CD40LG Recombinant Antibody, FITC (clone 4AM91) | FC, IF | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-027 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Ruplizumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-876 | Afuco™ Anti-CD40LG ADCC Recombinant Antibody (Toralizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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