Anti-Human ERBB2 Recombinant Antibody (Trastuzumab Emtansine) (CAT#: TAB-H70)

Recombinant Humanized (from mouse) antibody to Human ERBB2. Trastuzumab emtansine (ado-trastuzumab emtansine, trade name trastuzumab emtansine) is an antibody-drug conjugate consisting of the monoclonal antibody trastuzumab (trastuzumab) linked to the cytotoxic agent mertansine (DM1). Trastuzumab alone stops growth of cancer cells by binding to the HER2/neu receptor, whereas mertansine enters cells and destroys them by binding to tubulin. Because the monoclonal antibody targets HER2, and HER2 is only over-expressed in cancer cells, the conjugate delivers the toxin specifically to tumor cells. The conjugate is abbreviated T-DM1.


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Figure 1 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.

Figure 1 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.

Human epidermal growth factor receptor 2 (HER2) expression in N87 and N87-16-8 cells was analyzed by Western blotting.

Wang, H., Wang, W., Xu, Y., Yang, Y., Chen, X., Quan, H., & Lou, L. (2017). Aberrant intracellular metabolism of T‐DM 1 confers T‐DM 1 resistance in human epidermal growth factor receptor 2‐positive gastric cancer cells. Cancer science, 108(7), 1458-1468.

Figure 2 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.

Figure 2 Binding, internalization, and externalization of trastuzumab emtansine (T-DM1) are not significantly different between N87 and N8716-8 cells.

N87 and N87-16-8 cells were treated with 1 ug/mL DyLight 488 NHS Ester-labeled T-DM1 on ice for 1 h and washed three times with cold FACS buffer, after which binding of T-DM1 to cells was analyzed by flow cytometry.

Wang, H., Wang, W., Xu, Y., Yang, Y., Chen, X., Quan, H., & Lou, L. (2017). Aberrant intracellular metabolism of T‐DM 1 confers T‐DM 1 resistance in human epidermal growth factor receptor 2‐positive gastric cancer cells. Cancer science, 108(7), 1458-1468.


Specifications

  • Host Species
  • Mouse
  • Derivation
  • Humanized (from mouse)
  • Type
  • IgG1 - kappa
  • Specificity
  • ERBB2(epidermal growth factor receptor 2, receptor tyrosine-protein kinase erbB-2, EGFR2, HER2, HER-2, p185c-erbB2, NEU, CD340) [Homo sapiens]
  • Species Reactivity
  • Human
  • Applications
  • ELISA, IP, FC, FuncS, Neut, IF, ICC
  • Trade name
  • trastuzumab emtansine
  • CAS
  • 1018448-65-1
  • Generic Name
  • trastuzumab emtansine
  • Biological Half-Life
  • 4 days
  • ATC Code
  • L01XC14
  • UNII
  • SE2KH7T06F
  • MW
  • 148.5 kDa
  • Related Disease
  • Breast cancers overexpressing ERBB2, metastatic

Product Property

  • Purity
  • >95.0% as determined by analysis by RP-HPLC.

Applications

  • Application Notes
  • The ERBB2 antibody has been reported in applications of WB, FC.

Target

  • Alternative Names
  • trastuzumab emtansine;trastuzumab emtansine;1018448-65-1;PRO132365;trastuzumab-MCC-DM1;Trastuzumab-DM1;T-DM1;RG3502;trastuzumab emtansine;ado-trastuzumab emtansine;ERBB2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian);NG

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ERBB2"

Intrabody

CAT Product Name Application Type
IAB-B008(A) Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] IF, FC, FuncS scFv-(D-Arg)9
IAB-B008(G) Recombinant Anti-human ERBB2 Intrabody [+36 GFP] WB, ICC, FuncS scFv-(+36GFP)
IAB-B008(T) Recombinant Anti-human ERBB2 Intrabody [Tat] ICC, Neut, FuncS scFv-Tat

Chimeric Antibody

CAT Product Name Application Type
TAB-761 Anti-ERBB2 Recombinant Antibody (Margetuximab) IF, IP, Neut, FuncS, ELISA, FC, WB IgG1 - kappa
TAB-049CT Anti-Human HER2/neu Recombinant Antibody (ChA21) IHC, Inhib, WB, Activ, FC Chimeric antibody (mouse/human)
TAB-049CT-S(P) Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) WB Chimeric antibody (mouse/human)
TAB-049CT-F(E) Anti-Human HER2/neu Recombinant Antibody Fab Fragment (ChA21) WB Chimeric antibody (mouse/human)

Immunotoxin

CAT Product Name Application Type
AGTO-G022E Anti-ERBB2 immunotoxin 4D5 (scFv)-PE Cytotoxicity assay, Function study
AGTO-G022D Anti-ERBB2 immunotoxin 4D5 (scFv)-DT Cytotoxicity assay, Function study
AGTO-G022G Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel Cytotoxicity assay, Function study
AGTO-G022R Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA Cytotoxicity assay, Function study
AGTO-G022S Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap Cytotoxicity assay, Function study

Humanized Antibody

CAT Product Name Application Type
TAB-033CT Human Anti-ERBB2 Recombinant Antibody (TAB-033CT) ELISA, WB Human IgG1, κ
TAB-038CT Human Anti-ERBB2 Recombinant Antibody (TAB-038CT) FuncS, FC Humanized IgG1
TAB-039CT Anti-Human HER2/neu Recombinant Antibody (h1E11) WB Humanized antibody
TAB-041CT Human Anti-ERBB2 Recombinant Antibody (TAB-041CT) SPR, ELISA, WB Humanized antibody
TAB-050CT Anti-Human HER2/neu Recombinant Antibody (HuA21) Inhib, Cty, IHC, WB, IF, FC, ELISA Humanized antibody

Fab Glycosylation

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-177LC Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) ELISA Humanized antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0195MZ Chicken Anti-ERBB2 Polyclonal IgY WB Chicken antibody
BRD-0695MZ Chicken Anti-ERBB2 Polyclonal IgY WB Chicken antibody
BRD-0779MZ Chicken Anti-ErbB2 Polyclonal IgY ICC, IF, IHC, IP Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-737CQ Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) Block, IP, IF, FC Mouse IgG2a

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-468CQ Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Timigutuzumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-053 Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Pertuzumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-761 Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Margetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-005 Afuco™ Anti-ERBB2 ADCC Recombinant Antibody (Trastuzumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

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