Anti-Human IL4 Recombinant Antibody (Pascolizumab) (CAT#: TAB-221)

Figure 1 Effect of pascolizumab binding of IL‐4 inhibits IL‐4 interaction with the alpha chain of the IL‐4 receptor.

IL=interleukin; anti‐IL‐4=antibody against IL‐4; γ C=gamma signalling chain of the IL‐4 receptor; IL‐4Rα=IL‐4 receptor alpha; IL‐13Rα=IL‐13 receptor alpha.

Hart, T. K. , Blackburn, M. N. , Brigham-Burke, M. , Dede, K. , Al-Mahdi, N. , & Zia-Amirhosseini, P. , et al. (2010). Preclinical efficacy and safety of pascolizumab (sb 240683): a humanized anti‐interleukin‐4 antibody with therapeutic potential in asthma. Clin.exp.immunol, 130(1), 93-100.

Figure 2 Effect of MoAb 3B9 and pascolizumab on IL‐4‐dependent IgE synthesis from human PBMCs.

(a) and surface expression of CD23 on purified human tonsillar B cells (b). Results are expressed as percentage of the control response obtained with IL‐4‐stimulated cells. •, MoAb 3B9; , pascolizumab.

Hart, T. K. , Blackburn, M. N. , Brigham-Burke, M. , Dede, K. , Al-Mahdi, N. , & Zia-Amirhosseini, P. , et al. (2010). Preclinical efficacy and safety of pascolizumab (sb 240683): a humanized anti‐interleukin‐4 antibody with therapeutic potential in asthma. Clin.exp.immunol, 130(1), 93-100.

Figure 1 Anti-Human IL4 Recombinant Antibody (TAB-221) in ELISA

ELISA analysis of TAB-221 was performed by coating with human IL4 protein (His tag). Then blocked with BSA and incubated with TAB-221. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL4 Recombinant Antibody (TAB-221) in WB

Western blot analysis of TAB-221 was performed by loading human IL4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-221 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL4 Recombinant Antibody (TAB-221) in DB

Dot Blot analysis of TAB-221 was performed by coating with human IL4 protein (His tag).
TAB-221 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control