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Recombinant Mouse anti-Human MYC Monoclonal antibody (4H40) (CAT#: MOB-0268CT)

Recombinant mouse monoclonal antibody to human MYC, expressed in Chinese Hamster Ovary cells(CHO). This antibody recognizes proteins tagged with c-myc at either the C-terminus or the N-terminus as well as Human c-Myc protein. The immunogen of this product is a synthetic peptide corresponding to amino acids 408-439 of human c-Myc.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg
Figure 2 IF staining of human cell line SK-MEL-30
Figure 2 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg
Figure 3 IHC staining of human cerebellum
Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg
Figure 4 IHC staining of human rectum
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg
Figure 5 IHC staining of human caudate
Figure 5 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg
Figure 6 IF staining of human cell line PC-3
Figure 6 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg
Figure 7 IF staining of human cell line U-251 MG
Figure 7 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Figure 8 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg
Figure 9 Liver
Figure 9 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg
Figure 10 Kidney
Figure 10 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg
Figure 11 Cervix
Figure 11 Cervix
(Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg
Figure 12 Lymph node
Figure 12 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC

Specifications

  • Immunogen
  • Synthetic peptide corresponding to amino acids 408-439 of human c-Myc.
  • Host Species
  • Mouse
  • Specificity
  • This antibody recognizes proteins tagged with c-myc at either the C-terminus or the N-terminus as well as Human c-Myc protein.
  • Species Reactivity
  • Human
  • Clone
  • 4H40
  • Applications
  • Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Immunocytochemistry/Immunofluorescence; Immunohistochemistry paraffin embedded sections; Immunoprecipitation; Western Blot
  • Conjugate
  • FITC

Product Property

  • Purity
  • Protein A purified
  • Format
  • Liquid
  • Concentration
  • 0.5 mg/ml
  • Buffer
  • Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Storage
  • Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Target

  • Alternative Names
  • MYC; v-myc avian myelocytomatosis viral oncogene homolog; MRTL; MYCC; c-Myc; bHLHe39; myc proto-oncogene protein; proto-oncogene c-Myc; transcription factor p64; class E basic helix-loop-helix protein 39; avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; myc-related translation/localization regulatory factor;
  • Function
  • DNA binding; E-box binding; protein binding; sequence-specific DNA binding transcription factor activity;

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "MYC"

Recombinant Antibody

CAT Product Name Application Type
PABL-281 Mouse Anti-MYC Recombinant Antibody (clone 9E10) WB, IHC, FuncS Mouse IgG
ZG-0713F Mouse Anti-MYC Recombinant Antibody (clone 33) WB, IP, IHC Mouse IgG1
VS3-CJ230 Mouse Anti-MYC Recombinant Antibody (VS3-CJ230) WB, ELISA
VS3-CJ231 Rabbit Anti-MYC Recombinant Antibody (VS3-CJ231) WB, ICC, IF, FC Rabbit IgG
VS3-CJ232 Rabbit Anti-MYC Recombinant Antibody (VS3-CJ232) WB, ICC, IF, IHC, IP Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
PSBL-281 Mouse Anti-MYC Recombinant Antibody (clone 9E10); scFv Fragment WB, IHC, FuncS Mouse scFv
FAMAB-0112-CN-S(P) Mouse Anti-MYC Recombinant Antibody (clone CT14); scFv Fragment ELISA, WB, FC Mouse scFv

Fab Fragment Antibody

CAT Product Name Application Type
PFBL-281 Mouse Anti-MYC Recombinant Antibody (clone 9E10); Fab Fragment WB, IHC, FuncS Mouse Fab
FAMAB-0112-CN-F(E) Mouse Anti-MYC Recombinant Antibody (clone CT14); Fab Fragment ELISA, WB, FC Mouse Fab

Mouse Antibody

CAT Product Name Application Type
MOB-0400MC Anti-c-Myc antibody (HRP) ELISA, IHC-P, IHC-Fr, WB

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0380MZ Chicken Anti-c-Myc (ab1) Polyclonal IgY Indirect ELISA, WB Chicken antibody
BRD-0381MZ Chicken Anti-c-Myc (ab2) Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2336 Hi-Affi™ Recombinant Rabbit Anti-MYC Monoclonal Antibody (DS2336AB) WB, IP, ICC IgG
MOR-4554 Hi-Affi™ Recombinant Rabbit Anti-MYC Monoclonal Antibody (TH64DS) WB, IF, ICC, FC IgG
MOR-0050-FY Rabbit Anti-MYC Recombinant Antibody (clone AFY0021) ICC, IHC, WB Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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