Recombinant Mouse Anti-Huntingtin (Htt) Antibody (3B5H10) (CAT#: PABX-108)

Anti-Huntingtin (Htt) antibody is a Mouse antibody of IgG class that binds to an Huntingtin (Htt). This antibody recognizes a species of htt in neurons.


Specific Inquiry
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  • Endotoxin:
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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 Higher temperature sensitivity of the Q25 TR-FRET signal is due to structural or spatial changes of HTT polyQ.

Figure 1 Higher temperature sensitivity of the Q25 TR-FRET signal is due to structural or spatial changes of HTT polyQ.

(a) Western-blots of HEK293T cells transfected with different HTT-exon1 Q25 and Q72 constructs with point mutations as indicated. The last lane on each blot is from nontransfected HEK293T lysate. (b) Temperature sensitivity of the 4C9/MW1 TR-FRET signals. All signals are normalized to the signal at room temperature (RT), n53. Q25 has higher temperature sensitivity for all the HTT-exon1 constructs with or without point mutations. (c) Temperature sensitivity of the 2B7/3B5H10 TR-FRET signals for Q25 and Q72. All signals are normalized to the signal at room temperature (RT), n56. Between 4uC and RT, Q25 has higher temperature sensitivity than Q72.

Cui, X., Liang, Q., Liang, Y., Lu, M., Ding, Y., & Lu, B. (2014). TR-FRET assays of Huntingtin protein fragments reveal temperature and polyQ length-dependent conformational changes. Scientific reports, 4, 5601.

Figure 2 Monitoring disassembly of single htt aggregates incubated withMW7or 3B5H11 by in situ AFM.

Figure 2 Monitoring disassembly of single htt aggregates incubated withMW7or 3B5H11 by in situ AFM.

Samples of HD53Q were incubated for 6–8 h after removal of the GST moiety to form a large population of fibrils. These fibrils were deposited on mica and imaged using in situ AFM, which allows for the tracking of the fate of individual fibrils as a function of time. These fibrils were imaged in the absence or presence of anti-htt antibodies. Fibrils appeared to be stable after treatment with buffer, MW1-MW5, or MW8 (location of stable fibrils indicated by black arrows). However, treatment with MW7or 3B5H10 caused fibrils to disaggregate and/or shorten in length (location of disaggregating fibrils indicated by green arrows). Scale bar represents 500 nm and is applicable to all images.

Legleiter, J., Lotz, G. P., Miller, J., Ko, J., Ng, C., Williams, G. L., ... & Muchowski, P. J. (2009). Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. Journal of Biological Chemistry, 284(32), 21647-21658.

Figure 3 Size analysis of aggregate observed with MW7 or 3B5H10 added to monomeric or fibrillar HD54Q.

Figure 3 Size analysis of aggregate observed with MW7 or 3B5H10 added to monomeric or fibrillar HD54Q.

A, A, HD53Q oligomers (HD53Q incubated alone) after 5 h of incubation were predominantly 4 –5 nm in height with several as tall as 6 – 8 nm. B, when MW7 was incubated (added
at t = 0 h) with monomeric HD53Q (black diamonds), the height of globular aggregates formed after 5 h of co-incubation were predominantly 3– 4 nm tall, although there was a large portion of taller globular aggregates(shoulder on the right of the histogram). In contrast, when MW7 was incubated with pre-aggregated fibrillar HD53Q (gray circles), globular aggregates (conditions where fibrils disaggregated) observed when imaged 3 h after addition of MW7 were much taller (4 –5 nm) in comparison to those formed by adding MW7 to monomeric HD53Q, with a larger portion of aggregates being 5–10 nm tall. C, when 3B5H10 was incubated (added at t = 0 h) with monomeric HD53Q (black diamonds), the majority of globular aggregates observed after 5 h co-incubation were 2–3 nm in height. Similarly, when 3B5H10 was incubated with pre-aggregated fibrillar
HD53Q (gray circles), globular species (conditions where fibrils disaggregated) observed 3 h after the addition of 3B5H10 again were predominantly 2–3 nm tall.

Legleiter, J., Lotz, G. P., Miller, J., Ko, J., Ng, C., Williams, G. L., ... & Muchowski, P. J. (2009). Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. Journal of Biological Chemistry, 284(32), 21647-21658.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human Huntingtin (Htt)
  • Species Reactivity
  • Human
  • Clone
  • 3B5H10
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS (assay-depended)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • HTT; huntingtin; HD; IT15; huntington disease protein;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 3B5H10"

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Single-domain Antibody

CAT Product Name Application Type
NAB-1905-VHH Recombinant Anti-human HTT VHH Single Domain Antibody IHC, ICC, FC, FA, FUNC Llama VHH
PABJ-0201 Human Anti-HTT Recombinant Antibody (clone 3LRH) ELISA, FuncS Human VL

Intrabody

CAT Product Name Application Type
IAB-B023(A) Recombinant Anti-human HTT Intrabody [(D-Arg)9] ELISA, WB, ICC, FuncS scFv-(D-Arg)9
IAB-B023(G) Recombinant Anti-human HTT Intrabody [+36 GFP] IF, FC, WB, FuncS scFv-(+36GFP)
IAB-B023(T) Recombinant Anti-human HTT Intrabody [Tat] WB, CO-IP, FuncS scFv-Tat

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1714 Rabbit Anti-HTT Recombinant Antibody (clone DS1714AB) WB, IHC-P, ICC Rabbit IgG
MOR-0066-FY Rabbit Anti-HTT Recombinant Antibody (clone AFY0037) FC, ICC, IHC, WB Rabbit IgG

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