Recombinant monoclonal antibody specifically binds to IAV HA, expressed in Chinese Hamster Ovary cells(CHO).
Figure 1 Low pH induced conformational changes of H3 rHA were inhibited by the human serum samples.
Inhibition of low pH induced H3 rHA conformation change by a convenient human serum pool (Pool) in HCCIA. H3 rHAs bound to nickel-coated plates were treated with 100 μl of 200 ng/ml trypsin to cleave HA0 into HA1 and HA2. rHA coated plates were incubated with diluent only, 1:4000, 1:400, or 1:40 diluted Pool for 1 hour. The plate was washed and treated with a range of pH buffers followed by fixation with 0.05% glutaraldehyde/PBS. An ELISA was performed using a pH-specific mAb, HC31, and detected by measuring the OD at 450 nm.
Trost, J. F., LeMasters, E. H., Liu, F., Carney, P., Lu, X., Sugawara, K., ... & Katz, J. M. (2018). Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin. PloS one, 13(6), e0199683.
Figure 2 CCI showed virus neutralizing activity in TMN after removal of head-binding antibodies.
Correct folding of major epitopes for GH HA1 from A/Hong Kong/1/68 (H3N2) was confirmed by appropriate Ab binding profiles. GH HA1 from A/Hong Kong/1/68 (H3N2) and ectodomain H3 rHA from A/Aichi/1/68 (H3N2) were coated on a nickel-coated plate, an ELISA was performed by using rabbit antisera and a panel of conformation specific mAbs.
Trost, J. F., LeMasters, E. H., Liu, F., Carney, P., Lu, X., Sugawara, K., ... & Katz, J. M. (2018). Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin. PloS one, 13(6), e0199683.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
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• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
PFBL-206 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1F1) | WB, ELISA, FuncS | Fab |
PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABZ-076 | Recombinant Mouse Anti-IAV HA1 Antibody (26/9) | ELISA | IgG |
PABC-078 | Recombinant Mouse Anti-IAV HA (T131I) Antibody | Neut | IgG |
PABZ-078 | Recombinant Mouse Anti-IAV HA heptapeptide (DVPDYAS) Antibody (17/9) | ELISA | IgG |
PABL-482 | Recombinant Human Anti-H1N1 HA Antibody (CH65) | FuncS, Neut, WB | IgG |
PABL-483 | Recombinant Human Anti-H1N1 HA Antibody (CH67) | IF, FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-076 | Recombinant Mouse Anti-IAV HA1 Antibody scFv Fragment (26/9) | ELISA | scFv |
PSBC-078 | Recombinant Mouse Anti-IAV HA (T131I) Antibody scFv Fragment | Neut | scFv |
HPAB-M0099-YC-S(P) | Recombinant Human Anti-HA Antibody scFv Fragment (Ab 044) | ELISA, FuncS | Human scFv |
HPAB-M0100-YC-S(P) | Recombinant Human Anti-HA Antibody scFv Fragment (FI6) | ELISA, FuncS | Human scFv |
HPAB-S0058-YC-S(P) | Mouse Anti-HA Recombinant Antibody (clone HC19); scFv Fragment | Neut | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-268CT | Anti-IAV HA Recombinant Antibody (D6-1) | WB, FC, IP | Human antibody |
MRO-269CT | Anti-IAV HA Recombinant Antibody (IgA 59) | WB, Neut | Human antibody |
MRO-270CT | Anti-IAV HA Recombinant Antibody (IgA 119) | WB, Neut | Human antibody |
MRO-271CT | Anti-IAV HA Recombinant Antibody (IgA 340) | WB, Neut | Human antibody |
MRO-272CT | Anti-IAV HA Recombinant Antibody (IgG 134) | WB, Neut | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-303CT | Anti-IAV HA Recombinant Antibody (2242) | WB, ELISA | |
PABX-068 | Recombinant Mouse Anti-Hemagglutinin (HA) Antibody (1F1) | FuncS | IgG |
PABX-069 | Recombinant Mouse Anti-Hemagglutinin (HA) Antibody (S139/1) | Neut, FuncS | IgG |
PABX-070 | Recombinant Mouse Anti-HA1 Antibody (Fab17/9) | WB, ELISA, Neut, FuncS | IgG |
PABX-068-S (P) | Recombinant Mouse Anti-Hemagglutinin (HA) Antibody scFv Fragment (1F1) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-070-S (P) | Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) | WB, ELISA, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4209 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI287DS) | ELISA, ICC/IF, IF, FC | IgG |
MOR-4210 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI288DS) | ELISA, ICC/IF, IF, FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1169-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1169-FY) | Neut | Llama VHH |
HPAB-1170-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1170-FY) | Neut | Llama VHH |
HPAB-1171-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1171-FY) | Neut | Llama VHH |
HPAB-1172-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1172-FY) | Neut | Llama VHH |
HPAB-1173-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1173-FY) | Neut | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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