Recombinant Mouse Anti-IAV N9 NA Antibody (NC10) (CAT#: PABW-078)

Figure 1 Inhibition of wild-type and mutant NA activity by NC10.

Binding curve over the full range of antibody titration to show saturation. Cell lysate aliquots containing equal amounts of NA activity were preincubated with varying amounts of NC10; then enzyme activity was measured using fetuin as substrate. A549 readings were averaged for each antibody dilution used, and for wild-type, N329Q, S372A, N400L, K432M multiple experiments were averaged. Results are plotted as percent inhibition of NA activity.

Lee, J. T., & Air, G. M. (2002). Contacts between influenza virus N9 neuraminidase and monoclonal antibody NC10. Virology, 300(2), 255-268.

Figure 2 Inhibition of wild-type and mutant NA activity by NC10.

Expansion of the binding curves at low antibody concentrations to see the differences at 50% inhibition. Cell lysate aliquots containing equal amounts of NA activity were preincubated with varying amounts of NC10; then enzyme activity was measured using fetuin as substrate. A549 readings were averaged for each antibody dilution used, and for wild-type, N329Q, S372A, N400L, K432M multiple experiments were averaged. Results are plotted as percent inhibition of NA activity.

Lee, J. T., & Air, G. M. (2002). Contacts between influenza virus N9 neuraminidase and monoclonal antibody NC10. Virology, 300(2), 255-268.

Figure 3 ELISA measuring binding of mutant NAs to NC10.

The plates were coated with NC10 antibody and titrated with the cell lysates containing known amounts of NA. Bound NA was detected with anti-G70c NA rabbit antiserum and anti-rabbit IgG conjugated with alkaline phosphatase.

Lee, J. T., & Air, G. M. (2002). Contacts between influenza virus N9 neuraminidase and monoclonal antibody NC10. Virology, 300(2), 255-268.

Figure 4 Correlation between A405 ELISA reading and amount of NA measured by enzyme activity using the fluorogenic substrate 4-methylumbelliferyl-Nacetylneuraminic acid.

Lee, J. T., & Air, G. M. (2002). Contacts between influenza virus N9 neuraminidase and monoclonal antibody NC10. Virology, 300(2), 255-268.

Figure 5 Scatchard plots of inhibition of wild-type and mutant NAs by NC10.

B/F ratio of bound NC10 to free NC10. The total concentration of NA was from Western blot. The slope of the least-squares linear fit is equal to -1/K_d.

Lee, J. T., & Air, G. M. (2002). Contacts between influenza virus N9 neuraminidase and monoclonal antibody NC10. Virology, 300(2), 255-268.

Figure 6 Scatchard plots of the ELISA results to determine apparent K_d in the solid phase assay.

Lee, J. T., & Air, G. M. (2002). Contacts between influenza virus N9 neuraminidase and monoclonal antibody NC10. Virology, 300(2), 255-268.