Recombinant Human Anti-IFN-gamma Antibody (HuZAF) (CAT#: PABX-113)

Anti-IFN-gamma antibody is a Humanized antibody of IgG class that binds to an IFN-gamma.


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  • Published Data
  • Gene Expression
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Figure 1 Competitive binding analysis of AF2 and HuZAF.

Figure 1 Competitive binding analysis of AF2 and HuZAF.

A, Graph represents the relative ability of AF2 and HuZAF to compete with bAF2 for binding to human IFN-g in an ELISA format. Increasing concentrations of either AF2 (F) or HuZAF (■) were added to wells coated with human IFN-g, after which bAF2 was added. Error bars indicate the SE of triplicate samples. B, Neutralization of IFN-g-mediated induction of MHC class II
molecule expression on Hs294T cells. Graph represents the median fluorescent intensity of HLA-DR expression on Hs294T cells incubated with IFN-g and increasing concentrations of either AF2 (F) or HuZAF (■).
Median fluorescent intensity of Hs294T cells incubated in the absence of IFN-g was 3.74; median fluorescent intensity in the presence of IFN-g, but in the absence of Ab was 9.65.

Landolfi, N. F., Thakur, A. B., Fu, H., Vásquez, M., Queen, C., & Tsurushita, N. (2001). The integrity of the ball-and-socket joint between V and C domains is essential for complete activity of a humanized antibody. The Journal of Immunology, 166(3), 1748-1754.

Figure 2 Involvement of AF2 VH residues 11 and 38 in neutralization of IFN-g activity.

Figure 2 Involvement of AF2 VH residues 11 and 38 in neutralization of IFN-g activity.

Graph represents the median fluorescent intensity of HLA-DR expression on Hs294T cells incubated with IFN-g and increasing concentrations of either EuAF2 (F), or EuAF2 with the following substitutions: R38K (l), V11L (triangles), or V11L, R38K (■).

Landolfi, N. F., Thakur, A. B., Fu, H., Vásquez, M., Queen, C., & Tsurushita, N. (2001). The integrity of the ball-and-socket joint between V and C domains is essential for complete activity of a humanized antibody. The Journal of Immunology, 166(3), 1748-1754.

Figure 3 Stereo illustration of the three-dimensional structure of the HuZAF Fab, prepared with the program MOLSCRIPT (Kraulis, 1991).

Figure 3 Stereo illustration of the three-dimensional structure of the HuZAF Fab, prepared with the program MOLSCRIPT (Kraulis, 1991).

Secondary elements are superimposed on backbone tracings, which are colored yellow in the L chain and steel blue in the H chain. -Strands are represented by directional arrows: cyan for the four-stranded -pleated sheets and magenta for the ®ve-stranded (VL) and three-stranded layers (CL) of the L chain and red and white for the corresponding -pleated sheets in the H chain. Helices are identi®ed by red and yellow spirals. CDRs are marked 1, 2 and 3.

Bourne, P. C., Terzyan, S. S., Cloud, G., Landolfi, N. F., Vásquez, M., & Edmundson, A. B. (2004). Three-dimensional structures of a humanized anti-IFN-γ Fab (HuZAF) in two crystal forms. Acta Crystallographica Section D: Biological Crystallography, 60(10), 1761-1769.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human IFN-gamma
  • Species Reactivity
  • Human
  • Clone
  • HuZAF
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS (assay-depended)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • IFNG; interferon; gamma; IFG; IFI; interferon gamma; IFN-gamma; immune interferon; NI-0501

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Humanized Antibody

CAT Product Name Application Type
TAB-041 Anti-Human IFNG Recombinant Antibody (Fontolizumab) IP, IF, FuncS, FC, Neut, ELISA, IHC IgG1
PABX-113-F (E) Recombinant Human Anti-IFN-gamma Antibody Fab Fragment (HuZAF) WB, ELISA, FuncS Fab

Mouse Antibody

CAT Product Name Application Type
MOB-0289MC Recombinant Anti-Canine IFN-gamma Antibody. WB, ELISA

Neutralizing Antibody

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-0882LC Mouse Anti-IFNG Recombinant Antibody (clone MIF3009) ELISA Mouse IgG1

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-041 Afuco™ Anti-IFNG ADCC Recombinant Antibody (Fontolizumab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody
AFC-TAB-423CQ Afuco™ Anti-IFNG ADCC Recombinant Antibody (Emapalumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody

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