Recombinant Antagonist Human Antibody (MEDI546) is capable of binding to IFNAR1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IFNAR1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IFNAR1 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 Mean (±SD) serum MEDI-546 concentration.
A. Single IV Administration; B. Multiple IV Administrations. LLOQ, lower limit of quantitation; SD, standard deviation. Mean data below LLOQ are not plotted.
Goldberg, A., Geppert, T., Schiopu, E., Frech, T., Hsu, V., Simms, R. W.,... & Wang, B. (2014). Dose-escalation of human anti-interferon-α receptor monoclonal antibody MEDI-546 in subjects with systemic sclerosis: a phase 1, multicenter, open label study. Arthritis research & therapy,16(1), R57.
Figure 2 Membrane binding of A488-labelled anifrolumab to IFNAR1, and the APC-labelled membrane marker CD11b antibody, on primary human monocytes at 0 and 30min after placing cells under internalisation conditions of 37°C and 5% carbon dioxide (CO2).
Riggs, J. M., Hanna, R. N., Rajan, B., Zerrouki, K., Karnell, J. L., Sagar, D.,... & de los Reyes, M. (2018). Characterisation of anifrolumab, a fully human anti-interferon receptor antagonist antibody for the treatment of systemic lupus erythematosus.Lupus science & medicine, 5(1), e000261.
Figure 3 Anifrolumab inhibits type I interferon (IFN)-induced IFN-stimulated response element (ISRE) signalling.
Type I IFNinduced luciferase activity in HEK293-ISRE reporter cells elicited by (A) recombinant IFN-α2 protein (100 (triangles), 1000 (squares), 10 000 (circles) or 100 000 (diamonds) U/mL); (B and C) CpG-A-stimulated and DNA/IgG–immune complexes (DNAIC)-stimulated pDC supernatant (diluted 1:10 (triangles), 1:100 (squares) or 1:1000 (circles)), with preincubation with anifrolumab (black symbols) or isotype control antibody (white open circles). Type I IFN-induced luciferase activity in pIL5-ISRE reporter cells elicited by sera from patients with SLE with and without preincubation with anifrolumab or isotype control antibody; (D) representative assay with a single serum sample and anifrolumab IC50 of 0.05nM and (E) relative IFN activity of 20 serum samples. Mean IC50 for sera from three patients with SLE was 0.32±0.50nM.
Riggs, J. M., Hanna, R. N., Rajan, B., Zerrouki, K., Karnell, J. L., Sagar, D.,... & de los Reyes, M. (2018). Characterisation of anifrolumab, a fully human anti-interferon receptor antagonist antibody for the treatment of systemic lupus erythematosus.Lupus science & medicine, 5(1), e000261.
Figure 4 Anifrolumab inhibits cytokine production, plasmacytoid dendritic cell (pDC) activation and the type I (interferon gene signature) IFN gene signature.
IFN-α production by CpG-A-stimulated and DNA/IgG–immune complexes (DNA-IC)-stimulated (A) peripheral blood mononuclear cells (PBMCs) and (B) pDCs with and without preincubation with anifrolumab or isotype control antibody. Dose–response curve of antibody inhibition of IFN-α from a representative donor stimulated with (A and B, left panel) CpG-A or (A and B, centre panel) DNA-IC and (A and B, right panel) quantification of IFN inhibition in multiple donors at 10µg/mL (67.7 nM) anifrolumab or isotype control antibody. (C, left panel) Induction of the 21-gene IFN gene signature in CpG-A-stimulated PBMCs from three donors. (C, right panel) Inhibition of the 21-gene IFN gene signature in CpG-A-stimulated PBMCs from seven donors with and without preincubation with anifrolumab or isotype control antibody. (D) CD80 activation and CD83 maturation surface marker expression in CpG-A-stimulated pDCs with and without preincubation with 10µg/mL (67.7 nM) anifrolumab or isotype control antibody. (E) TNF-α, interleukin (IL)-6 and IL-8 secretion from CpG-A-stimulated pDCs with and without preincubation with 10 µg/mL (67.7 nM) anifrolumab or isotype control antibody.
Riggs, J. M., Hanna, R. N., Rajan, B., Zerrouki, K., Karnell, J. L., Sagar, D.,... & de los Reyes, M. (2018). Characterisation of anifrolumab, a fully human anti-interferon receptor antagonist antibody for the treatment of systemic lupus erythematosus.Lupus science & medicine, 5(1), e000261.
Figure 5 Anifrolumab suppresses plasmacytoid dendritic cell (pDC)-dependent plasma cell differentiation.
(A) Representative flow cytometry plots and quantification of plasma cell (CD19+ CD27highCD38high) differentiation in untreated, interleukin (IL)-21- stimulated (positive control) or CpG-A-stimulated B cell/pDC co-cultures. (B) Representative flow cytometry plot and (C and D) quantification of plasma cell differentiation in CpG-A-stimulated B cell/pDC co-cultures with and without preincubation with anifrolumab or control antibody.
Riggs, J. M., Hanna, R. N., Rajan, B., Zerrouki, K., Karnell, J. L., Sagar, D.,... & de los Reyes, M. (2018). Characterisation of anifrolumab, a fully human anti-interferon receptor antagonist antibody for the treatment of systemic lupus erythematosus.Lupus science & medicine, 5(1), e000261.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1305z | Mouse Anti-IFNAR1 Recombinant Antibody (MOB-1305z) | ELISA, WB, IP, IF, IHC, FC | Mouse IgG1, κ |
HPAB-0158-CN | Human Anti-IFNAR1 Recombinant Antibody (clone S3A5) | FC | Human IgG |
HPAB-0159-CN | Human Anti-IFNAR1 Recombinant Antibody (clone S5B4) | FC | Human IgG |
HPAB-0160-CN | Human Anti-IFNAR1 Recombinant Antibody (clone AmS3A5-1) | ELISA | Human IgG1, κ |
HPAB-0161-CN | Human Anti-IFNAR1 Recombinant Antibody (clone AmS3A5-2) | ELISA | Human IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1305z-S(P) | Recombinant Anti-Human IFNAR1 Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
PSBL-228 | Human Anti-IFNAR1 Recombinant Antibody (clone MEDI546); scFv Fragment | WB, ELISA, FuncS | Human scFv |
HPAB-0158-CN-S(P) | Human Anti-IFNAR1 Recombinant Antibody (clone S3A5); scFv Fragment | ELISA | Human scFv |
HPAB-0159-CN-S(P) | Human Anti-IFNAR1 Recombinant Antibody (clone S5B4); scFv Fragment | ELISA | Human scFv |
HPAB-0160-CN-S(P) | Human Anti-IFNAR1 Recombinant Antibody (clone AmS3A5-1); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-130CT | Anti-Human IFNAR-1 Recombinant Antibody (64G12) | WB, IF, FuncS | |
TAB-130CT-S(P) | Anti-Human IFNAR-1 Recombinant Antibody scFv Fragment (64G12) | ELISA, FC | |
TAB-130CT-F(E) | Anti-Human IFNAR-1 Recombinant Antibody Fab Fragment (64G12) | ELISA, FC |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-131CT | Anti-Human IFNAR-1 Recombinant Antibody (CPI-1697) | ELISA, FC | Humanized antibody |
TAB-131CT-S(P) | Anti-Human IFNAR-1 Recombinant Antibody scFv Fragment (CPI-1697) | ELISA, FC | Humanized antibody |
TAB-131CT-F(E) | Anti-Human IFNAR-1 Recombinant Antibody Fab Fragment (CPI-1697) | ELISA, FC | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-976CQ | Mouse Anti-Ifnar1 Recombinant Antibody (clone MAR1-5A3) | FC, Neut, IP, WB, B | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1740 | Rabbit Anti-IFNAR1 Recombinant Antibody (clone DS1740AB) | WB, FC, IP | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0158-CN-F(E) | Human Anti-IFNAR1 Recombinant Antibody (clone S3A5); Fab Fragment | ELISA | Human Fab |
HPAB-0159-CN-F(E) | Human Anti-IFNAR1 Recombinant Antibody (clone S5B4); Fab Fragment | ELISA | Human Fab |
HPAB-0160-CN-F(E) | Human Anti-IFNAR1 Recombinant Antibody (clone AmS3A5-1); Fab Fragment | ELISA | Human Fab |
HPAB-0161-CN-F(E) | Human Anti-IFNAR1 Recombinant Antibody (clone AmS3A5-2); Fab Fragment | ELISA | Human Fab |
HPAB-0203CQ-F(E) | Human Anti-IFNAR1 Recombinant Antibody (clone H3K1); Fab Fragment | ELISA, FC, Inhib | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-722 | Afuco™ Anti-IFNAR ADCC Recombinant Antibody (Anifrolumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
There are currently no Customer reviews or questions for PABL-228. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.