Human Anti-IFNG Recombinant Antibody (clone AF-2) (CAT#: PABW-079)

Figure 1 Effect of AF-2, a neutralizing Ab to human IFN-γ, on PMA-induced E-selectin expression on HUVEC, pretreated with IFN-γ.

HUVEC were cultured with 200 ng/ml IFN-γ ▪ or medium alone □ for 24 h in the presence or absence of the indicated Abs at 10 μg/ml. PMA, 1 ng/ml, was then added for an additional 4 h, and cells were analyzed for expression of E-selectin by a cell-based ELISA, as described in Materials and Methods. The relative amount of E-selectin measured is indicated by the OD at 450 nm. The mean ± SD from triplicate samples in a single, representative experiment is shown. **Indicates p < 0.005; *indicates p < 0.05; NS indicates not significant, comparing with and without IFN-γ.

Melrose, J. , Tsurushita, N. , Liu, G. , & Berg, E. L. . (1998). Ifn-γ inhibits activation-induced expression of e- and p-selectin on endothelial cells. Journal of Immunology, 161(5), 2457-64.