Anti-Influenza A Virus H1N1 Hemagglutinin HA1 subunit Recombinant Antibody (R2a-G9) (CAT#: MRO-246LC-VHH)

This product is a recombinant alpaca anti-influenza A virus H1N1 single domain antibody. R2a-G9 specifically binds to hemagglutinin HA1 subunit and can be potentially usd in the treatment of influenza virus infection.


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ELISA

Figure 1 Specificity of single domain antibodies to different influenza antigen reference reagents.

Figure 1 Specificity of single domain antibodies to different influenza antigen reference reagents.

ELISA comparing binding of purified VHH antibodies at 30 mg/ml against H1N1, seasonal H1N1 and H5N1.

Hufton, S. E., Risley, P., Ball, C. R., Major, D., Engelhardt, O. G., & Poole, S. (2014). The breadth of cross sub-type neutralisation activity of a single domain antibody to influenza hemagglutinin can be increased by antibody valency. PLoS One, 9(8), e103294.

SPR

Figure 2 Antibody affinity on recombinant HA by surface plasmon resonance.

Figure 2 Antibody affinity on recombinant HA by surface plasmon resonance.

Affinity on recombinant H1-HA, H1N1.

Hufton, S. E., Risley, P., Ball, C. R., Major, D., Engelhardt, O. G., & Poole, S. (2014). The breadth of cross sub-type neutralisation activity of a single domain antibody to influenza hemagglutinin can be increased by antibody valency. PLoS One, 9(8), e103294.

FuncS

Figure 3 Viral neutralisation activity of cross-reactive sdAbs.

Figure 3 Viral neutralisation activity of cross-reactive sdAbs.

Hufton, S. E., Risley, P., Ball, C. R., Major, D., Engelhardt, O. G., & Poole, S. (2014). The breadth of cross sub-type neutralisation activity of a single domain antibody to influenza hemagglutinin can be increased by antibody valency. PLoS One, 9(8), e103294.

ELISA

Figure 4 Characterisation of antibody epitopes.

Figure 4 Characterisation of antibody epitopes.

ELISA showing reactivity of purified antibodies at 30 mg/ml to HA antigen standard H1N1 either treated with low pH or neutral pH.

Hufton, S. E., Risley, P., Ball, C. R., Major, D., Engelhardt, O. G., & Poole, S. (2014). The breadth of cross sub-type neutralisation activity of a single domain antibody to influenza hemagglutinin can be increased by antibody valency. PLoS One, 9(8), e103294.

ELISA

Figure 5 Characterisation of antibody epitopes.

Figure 5 Characterisation of antibody epitopes.

ELISA showing binding of phage displayed HA gene fragments to purified sdAbs.

Hufton, S. E., Risley, P., Ball, C. R., Major, D., Engelhardt, O. G., & Poole, S. (2014). The breadth of cross sub-type neutralisation activity of a single domain antibody to influenza hemagglutinin can be increased by antibody valency. PLoS One, 9(8), e103294.

SPR

Figure 6 Grouping of antibody epitopes.

Figure 6 Grouping of antibody epitopes.

SPR co-injection experiments were used to determine if pairs of VHH antibodies can bind to recombinant H1-HA simultaneously. The antibodies R1a-A5, R1a-B6, R2b-D9, R2b-E8, R1a-C5 and R2a-G9 were tested in combination with each other. Example series of sensorgrams of R2b-D9 injected as the first antibody followed by injection of each of the other antibodies as indicated R2b-D9/R2a-G9.

Hufton, S. E., Risley, P., Ball, C. R., Major, D., Engelhardt, O. G., & Poole, S. (2014). The breadth of cross sub-type neutralisation activity of a single domain antibody to influenza hemagglutinin can be increased by antibody valency. PLoS One, 9(8), e103294.


Specifications

  • Host Species
  • Alpaca
  • Derivation
  • Phage display library
  • Type
  • Single domain antibody
  • Specificity
  • Influenza A virus H1N1
  • Clone
  • R2a-G9
  • Applications
  • ELISA, FuncS, SPR
  • Related Disease
  • Influenza virus infections

Applications

  • Application Notes
  • The antibody was validated for ELISA, Functional Assay, Surface plasmon resonance. For details, refer to Published Data.

Target

  • Alternative Names
  • Influenza A Virus H1N1 Hemagglutinin HA1 subunit

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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