Mouse Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) (CAT#: MRO-280LC)

Figure 1 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in SDS-PAGE

SDS-PAGE analysis of MRO-280LC in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-245 kDa.

Figure 2 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in SEC-HPLC

SEC-HPLC analysis of MRO-280LC shows that the purity is more than 95%.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in ELISA

ELISA analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with MRO-280LC. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in ELISA

ELISA analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with MRO-280LC. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 5 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in WB

Western blot analysis of MRO-280LC was performed with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MRO-280LC and HRP goat anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.8 μg)

Figure 6 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in WB

Western blot analysis of MRO-280LC was performed with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MRO-280LC and HRP goat anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.8 μg)

Figure 7 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in DB

Dot Blot analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).

MRO-280LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 8 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in DB

Dot Blot analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).

MRO-280LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)