This product is a recombinant anti-Japanese encephalitis virus monoclonal antibody 2B4. 2B4 specifically targets JEV and can be potentially used in the treatment study and diagnosis of Japanese encephalitis virus infection.
Figure 1 The mAb 2B4 recognized JEV-infected BHK-21 cells.
The cells were infected with JEV NJ-2008 strain. At 72 hpi, the cells were fixed and primed with mAb 2B4 (1) and rabbit polyclonal antibody (pAb) (2), respectively; uninfected BHK-21cells, CSFV-infected PK-15 cells, PRRSV-infected Marc-145cells, and PCV2-infected PK-15 cells were also primed with mAb 2B4 as the negative controls (3–6).
Fine mapping of a linear epitope on EDIII of Japanese encephalitis virus using a novel neutralizing monoclonal antibody
Figure 2 Identification of mAb 2B4 binding to JEV viral proteins by ELISA and Western blot analyses.
mAb 2B4 reacted with both rEDIII and rMEP proteins and the results showed a single band, respectively.
Fine mapping of a linear epitope on EDIII of Japanese encephalitis virus using a novel neutralizing monoclonal antibody
Figure 3 Identification of mAb 2B4 binding to JEV viral proteins by ELISA and Western blot analyses.
The ELISA data showed that mAb 2B4 had strong interactions with rEDIII and Rmep.
Fine mapping of a linear epitope on EDIII of Japanese encephalitis virus using a novel neutralizing monoclonal antibody
Figure 4 The PRNT result of mAb 2B4.
The data showed that a PRNT50 titer of mAb 2B4 was 0.5 g/mL.
Fine mapping of a linear epitope on EDIII of Japanese encephalitis virus using a novel neutralizing monoclonal antibody
Figure 5 The survival curve of mice in the analysis of mAb neutralization of JEV.
The mice were monitored daily for 30 days. The survival percentages were shown and the numbers of mice (N) in each experimental group were shown in the legend to each panel. Each descent means one occurrence of death in mice. Survival curve comparisons were performed using Graphpad Prism 5.0 software statistical analysis.
Fine mapping of a linear epitope on EDIII of Japanese encephalitis virus using a novel neutralizing monoclonal antibody
Figure 6 Identification of the displayed epitopes by the phage displayed peptide library.
MAb recognition of clones selected from the phage displayed peptide library. Ten clones selected after three rounds of biopanning from phage display peptide library were tested for binding to mAb by phage ELISA. C1–C10 for binding to mAb 2B4; the anti-CSFV mAb WH303 served as a negative control.
Fine mapping of a linear epitope on EDIII of Japanese encephalitis virus using a novel neutralizing monoclonal antibody
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0158MZ | Recombinant Mouse Anti-Japanese Encephalitis Antibody (clone C926M) | ELISA, IF | Mouse antibody |
PABC-364 | Recombinant Mouse Anti-JEV Antibody (2F2) | Neut, IF, WB, ELISA | Mouse IgG |
PABC-365 | Recombinant Mouse Anti-JEV Antibody (2H4) | WB, Neut, ELISA | Mouse IgG |
HPAB-1276-FY | Human Anti-JEV Recombinant Antibody (HPAB-1276-FY) | ELISA, Neut, WB | Human IgG1 |
HPAB-1277-FY | Human Anti-JEV Recombinant Antibody (HPAB-1277-FY) | ELISA, Neut, WB | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-1162LC-S(P) | Anti-JEV Recombinant Antibody scFv Fragment (2B4) | ELISA, WB | Mouse Antibody |
MRO-1162LC-F(E) | Anti-JEV Recombinant Antibody Fab Fragment (2B4) | ELISA, WB | Mouse Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0335LC | Recombinant Mouse Anti-JEV Antibody (2H2) | WB | IgG3 |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBC-364 | Recombinant Mouse Anti-JEV Antibody Fab Fragment (2F2) | ELISA, Neut | Mouse Fab |
PFBC-365 | Recombinant Mouse Anti-JEV Antibody Fab Fragment (2H4) | ELISA, Neut | Mouse Fab |
HPAB-1276-FY-S(P) | Human Anti-JEV Recombinant Antibody; scFv Fragment (HPAB-1276-FY-S(P)) | WB, ELISA | Humanized scFv |
HPAB-1277-FY-S(P) | Human Anti-JEV Recombinant Antibody; scFv Fragment (HPAB-1277-FY-S(P)) | WB, ELISA | Human scFv |
HPAB-1278-FY-S(P) | Human Anti-JEV Recombinant Antibody; scFv Fragment (HPAB-1278-FY-S(P)) | WB, ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBC-364 | Recombinant Mouse Anti-JEV Antibody scFv Fragment (2F2) | ELISA, Neut | Mouse scFv |
PSBC-365 | Recombinant Mouse Anti-JEV Antibody scFv Fragment (2H4) | ELISA, Neut | Mouse scFv |
HPAB-1276-FY-F(E) | Human Anti-JEV Recombinant Antibody; Fab Fragment (HPAB-1276-FY-F(E)) | WB, ELISA | Humanized Fab |
HPAB-1277-FY-F(E) | Human Anti-JEV Recombinant Antibody; Fab Fragment (HPAB-1277-FY-F(E)) | WB, ELISA | Human Fab |
HPAB-1278-FY-F(E) | Human Anti-JEV Recombinant Antibody; Fab Fragment (HPAB-1278-FY-F(E)) | WB, ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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