Recombinant Human Anti-Jag1 Antibody scFv Fragment (JAG2.b33) (CAT#: PABX-126-S (P))

Anti-Jag1 antibody is a Human of scFv class that binds to an Jag1.


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Figure 1 Anti-JAG1.b70 and anti-JAG2.b33 specifically block signalling induced by their cognate ligands.

Figure 1 Anti-JAG1.b70 and anti-JAG2.b33 specifically block signalling induced by their cognate ligands.

a, ELISA measuring antibody binding to purified protein fragments of human (h) and murine (m) canonical Notch ligands.

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.

Figure 2 Anti-JAG1.b70 and anti-JAG2.b33 specifically block signalling induced by their cognate ligands.

Figure 2 Anti-JAG1.b70 and anti-JAG2.b33 specifically block signalling induced by their cognate ligands.

Side-view of anti-JAG1 Fab bound to the human JAG1 DSL–EGF1 region. JAG1 residues within 4.5Å of the Fab fragment (HC, heavy chain; LC, light chain) paratope are pink or yellow, with yellow designating residues unique to JAG1, relative to JAG2. Inset shows an ionic interaction between Asp204 (hJAG1) and His35 (HC) that anchors the JAG1–Fab interaction.

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.

Figure 3  Immunofluorescent staining for the club cell marker CC10 (green) and the ciliated cell marker acetylated-α-tubulin (acet-α-Tub; red) in distal airway epithelium.

Figure 3 Immunofluorescent staining for the club cell marker CC10 (green) and the ciliated cell marker acetylated-α-tubulin (acet-α-Tub; red) in distal airway epithelium.

Nuclei counterstained with DAPI. c, Quantification of club and ciliated cell numbers as assessed by immunofluorescent staining for CC10 and FOXJ1, respectively (percentages of each cell type over the total number of airway cells counted, n=5; unpaired t-test, **P<0.01, ***P<0.001).

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.

Figure 4 JAG blockade reverses goblet cell metaplasia in vivo.

Figure 4 JAG blockade reverses goblet cell metaplasia in vivo.

a, Mice were sensitized during a 34-day period after intraperitoneal (i.p.) injection of ovalbumin (OVA) or vehicle (non-sensitized, b) and then challenged with inhaled ovalbumin to induce inflammation and goblet cell metaplasia. D, day. c–f, Alcian blue/periodic acid-Schiff (PAS) staining for mucin from lung sections of sensitized mice treated with control antibody at the first (day 42) (c) or second (day 48) (d) dose of control antibody, or at the second dose of anti-JAG1.b70.

Lafkas, D., Shelton, A., Chiu, C., de Leon Boenig, G., Chen, Y., Stawicki, S. S., ... & Eastham-Anderson, J. (2015). Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung. Nature, 528(7580), 127.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • scFv
  • Specificity
  • Tested positive against native Human Huntingtin (Htt)
  • Species Reactivity
  • Human
  • Clone
  • JAG2. b33
  • Applications
  • Can be useful in applications such as: Western blot; Neutralization; Functional Study

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • AGS; AHD; AWS; HJ1; CD339; JAGL1

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone JAG2. b33"

See other products for "JAG1"

Human Antibody

CAT Product Name Application Type
PABX-126-F (E) Recombinant Human Anti-Jag1 Antibody Fab Fragment (JAG2.b33) WB, Neut, FuncS Fab

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1895 Hi-Affi™ Recombinant Rabbit Anti-JAG1 Monoclonal Antibody (DS1895AB) WB, IHC-P, ICC/IF IgG

Neuroscience Antibody

CAT Product Name Application Type
NS-049CN Human Anti-JAG1 Recombinant Antibody (NS-049CN) ELISA, WB, IF Human IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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