Recombinant Human Antibody (Fab19) is capable of binding to Kit, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Kit mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Kit mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 NIH 3T3 cells expressing WT KIT were incubated for 5 h with the indicated concentrations (Upper) of Fabs or IgGs following stimulation with 25 ng/mL of SCF for 5 min at 37℃. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation (IP) with anti-KIT antibodies followed by SDS/PAGE and immunoblotting (IB) with anti-KIT or anti-phosphotyrosine (pY) antibodies.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M.,... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences,110(44), 17832-17837.
Figure 2 (B and C) Ba/F3 cells expressing WT KIT were plated in six-well plates and grown in the presence of 250 ng/mL SCF and different concentrations as indicated (Lower) of Fabs (B) or IgGs (C). Cell numbers were measured after 72 h relative to 0 h time points.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M.,... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences,110(44), 17832-17837.
Figure 3 Proliferation of Ba/F3 cells expressing A502,Y503 duplication oncogenic KIT mutant is inhibited by anti-D4 antibodies. Ba/F3 cells expressing the dupA502,Y503 KIT mutant were plated in 6 well plates. Cells were grown in the presence of different concentrations of Fabs (A) or IgGs (B), in the absence of IL-3 or SCF (as indicated). Folds increase of cell number was measured after 72 hours.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-470CL | Human Anti-KIT Recombinant Antibody (TAB-470CL) | ELISA, WB | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-450CL | Afuco™ Anti-KIT Recombinant Antibody (AFC-450CL), ADCC Enhanced | ELISA, WB | Humanized IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-255 | Human Anti-KIT Recombinant Antibody (clone Fab19); scFv Fragment | WB, ELISA, FuncS | Human scFv |
HPAB-3398LY-S(P) | Human Anti-KIT Recombinant Antibody (clone 37M); scfv Fragment | ELISA, FC, WB | Human scfv |
HPAB-1948-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1948-FY-S(P)) | Block, ELISA, FC, IF | Humanized scFv |
HPAB-1949-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1949-FY-S(P)) | Block, ELISA, FC, IF | Humanized scFv |
HPAB-1950-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1950-FY-S(P)) | Block, ELISA, FC, IF | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-255 | Human Anti-KIT Recombinant Antibody (clone Fab19); Fab Fragment | WB, ELISA, FuncS | Human Fab |
HPAB-0019-FY-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-0019-FY-F(E)) | FC | Humanized Fab |
HPAB-1570-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1570-FY-S(P)) | WB, ELISA | Human scFv |
HPAB-1571-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1571-FY-S(P)) | WB, ELISA | Human scFv |
HPAB-1572-FY-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (HPAB-1572-FY-S(P)) | WB, ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-467LC | Anti-Human KIT Recombinant Antibody (SR1) | WB | |
TAB-467LC-S(P) | Anti-Human KIT Recombinant Antibody scFv Fragment (SR1) | WB | |
TAB-467LC-F(E) | Anti-Human KIT Recombinant Antibody Fab Fragment (SR1) | WB |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-465LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-465LC-S(P)) | FC, WB, SPR, ELISA | Human scFv |
TAB-466LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-466LC-S(P)) | FC, WB, SPR, ELISA | Human scFv |
TAB-468LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-468LC-S(P)) | FC, WB, ELISA | Human scFv |
TAB-465LC-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (TAB-465LC-F(E)) | FC, WB, SPR, ELISA | Human Fab |
TAB-466LC-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (TAB-466LC-F(E)) | FC, WB, SPR, ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0316MZ | Chicken Anti-KIT Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1629CQ | Recombinant Mouse Anti-KIT Antibody (A3C6E2) | FC, BL | IgG1, κ |
NEUT-1633CQ | Recombinant Rat Anti-Kit Antibody (ACK2) | FC, BL, Depletion | IgG2b, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1630CQ | Recombinant Mouse Anti-KIT Antibody (CBL788) | WB, FC, CyTOF, ELISA(Cap), Neut | IgG1 |
NEUT-1631CQ | Recombinant Mouse Anti-KIT Antibody (14G5) | Neut, WB | IgG1 |
NEUT-1632CQ | Recombinant Mouse Anti-KIT Antibody (12A8) | WB, IP, FC, Neut | IgG1 |
NEUT-1634CQ | Recombinant Mouse Anti-KIT Antibody (CBL158) | Neut, WB | IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1948 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (DS1948AB) | IHC-P, IHC-Fr | IgG |
MOR-4551 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH61DS) | WB, IF, ICC | IgG |
MOR-4552 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH62DS) | WB, IF, ICC, FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-0366-CN | Recombinant Rabbit Anti-KIT Monoclonal Antibody (CBACN-149) | WB, IHC | Rabbit IgG |
HPAB-N0156-YC | Mouse Anti-KIT Recombinant Antibody (HPAB-N0156-YC) | ELISA, Inhib, FuncS | Mouse IgG |
HPAB-N0157-YC | Human Anti-KIT Recombinant Antibody (HPAB-N0157-YC) | ELISA, Inhib, FuncS | Humanized IgG1 |
HPAB-3398LY | Human Anti-KIT Recombinant Antibody (clone 37M) | ELISA, FC, WB | Human IgG |
HPAB-3399LY | Human Anti-KIT Recombinant Antibody (clone 37C) | ELISA, FC, WB | Human IgG |
There are currently no Customer reviews or questions for PABL-255. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.