Anti-Human L1CAM Recombinant Antibody (L1-9.3) (CAT#: TAB-0636CL)

This recombinant monoclonal antibody L1-9.3 is a mouse monoclonal antibody that specifically binds to human L1CAM. It is recommended for the detection of human L1CAM by ELISA, WB, IHC, and FC.


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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Characterization of novel mAb to L1.

Figure 1 Characterization of novel mAb to L1.

Western blot analysis of cell lysate from the indicated cells.

Wolterink, S., Moldenhauer, G., Fogel, M., Kiefel, H., Pfeifer, M., Lüttgau, S., ... & Altevogt, P. (2010). Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer research, 70(6), 2504-2515.

IHC

Figure 2 Characterization of novel mAb to L1.

Figure 2 Characterization of novel mAb to L1.

Immunohistochemical staining of human ovarian carcinoma tissues with anti-L1 mAbs.

Wolterink, S., Moldenhauer, G., Fogel, M., Kiefel, H., Pfeifer, M., Lüttgau, S., ... & Altevogt, P. (2010). Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer research, 70(6), 2504-2515.

ELISA

Figure 3 Characterization of novel mAb to L1.

Figure 3 Characterization of novel mAb to L1.

The purified Fc protein was coated, and mAb binding was analyzed by ELISA.

Wolterink, S., Moldenhauer, G., Fogel, M., Kiefel, H., Pfeifer, M., Lüttgau, S., ... & Altevogt, P. (2010). Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer research, 70(6), 2504-2515.

ELISA

Figure 4 Anti-L1 mAbs to the intracellular but not extracellular domain cross-react with human CHL1.

Figure 4 Anti-L1 mAbs to the intracellular but not extracellular domain cross-react with human CHL1.

Purified CHL1-Fc was tested in ELISA for cross-reactivity with anti-L1 mAbs. L1-Fc was used as positive control and POX-conjugated goat anti-human Fc for equal coating.

Wolterink, S., Moldenhauer, G., Fogel, M., Kiefel, H., Pfeifer, M., Lüttgau, S., ... & Altevogt, P. (2010). Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer research, 70(6), 2504-2515.

FC

Figure 5 Characterization of humanized L1-9.3 mAb.

Figure 5 Characterization of humanized L1-9.3 mAb.

Wolterink, S., Moldenhauer, G., Fogel, M., Kiefel, H., Pfeifer, M., Lüttgau, S., ... & Altevogt, P. (2010). Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer research, 70(6), 2504-2515.


Specifications

  • Host Species
  • Mouse
  • Specificity
  • Human
  • Clone
  • L1-9.3
  • Applications
  • WB, IHC, ELISA, FC
  • Related Disease
  • Tumorigenic disease

Applications

  • Application Notes
  • The L1CAM antibody has been reported in applications of Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Flow Cytometry.

Target

  • Alternative Names
  • L1CAM; L1 cell adhesion molecule; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1; neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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