Llama Anti-human LC-kappa VHH Single Domain Antibody [Biotin] (CAT#: NABG-118)

Anti-LC-kappa VHH Single Domain Antibody [Biotin]


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WB

Figure 1 Llama Anti-human LC-kappa VHH Single Domain Antibody (VHH-His-Cys) (NABG-118-UN) in WB

Figure 1 Llama Anti-human LC-kappa VHH Single Domain Antibody (VHH-His-Cys) (NABG-118-UN) in WB

Western blot analysis of NABG-118-UN was performed with Human IgG1, kappa antibody onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with NABG-118-UN and HRP Mouse Anti-His tag IgG as a secondary antibody. Chemiluminescent detection was performed.

ELISA

Figure 2 Llama Anti-human LC-kappa VHH Single Domain Antibody (VHH-His-Cys) (NABG-118-UN) in ELISA

Figure 2 Llama Anti-human LC-kappa VHH Single Domain Antibody (VHH-His-Cys) (NABG-118-UN) in ELISA

ELISA analysis of NABG-118-UN was performed by coating with Human IgG1, kappa. Then blocked with BSA, and incubated with NABG-118-UN. The HRP-conjugated mouse anti-His tag IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

DB

Figure 3 Llama Anti-human LC-kappa VHH Single Domain Antibody (VHH-His-Cys) (NABG-118-UN) in DB

Figure 3 Llama Anti-human LC-kappa VHH Single Domain Antibody (VHH-His-Cys) (NABG-118-UN) in DB

Antigen: Human IgG1, kappa antibody
Antibody incubation concentration: 2ng/μL.


Specifications

  • Immunogen
  • Human LC-kappa
  • Host Species
  • Llama
  • Species Reactivity
  • Chimpanzee, Rhesus, Cynomolgus
  • Applications
  • This product is suitable for use in ELISA, WB, BLI, SPR and most other immunological methods.
  • Conjugate
  • Biotin

Product Property

  • Purity
  • Purity >97% by ELISA and WB.
  • Buffer
  • 1 mg/mL protein in PBS, pH 7.4 (no preservatives added)
  • Storage
  • • 4°C for up to 1 month
    • –20°C for up to 1 year (aliquot to prevent repeated thaw/freeze steps)

Target

  • Alternative Names
  • LC-kappa; LC-κ; κ; LC; immunoglobulin light chain; immunoglobulin light chain kappa

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Single-domain Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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