Recombinant Mouse Anti-M2e Antibody (MAb148) (CAT#: PABX-140)

Anti-M2e antibody is a Mouse antibody of IgG1 class that binds to an M2e.


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Figure 1 Properties of binding of MAb 65 to M2e peptide and M2-expressing cells.

Figure 1 Properties of binding of MAb 65 to M2e peptide and M2-expressing cells.

(A) Binding of MAb 65 and MAb 148 to different M2e peptides. ELISA plates were coated with wild-type (WT), M2eW15G, or M2eP10L M2e peptides at 20 ng/ml or 200 ng/ml.

Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H., ... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.

Figure 2 Western blot analysis of cell lysates prepared 24 h after transfection to evaluate expression of NP, M2.

Figure 2 Western blot analysis of cell lysates prepared 24 h after transfection to evaluate expression of NP, M2.

Western blot analysis of cell lysates prepared 24 h after transfection to evaluate expression of NP, M2 (revealed by staining with MAb 148 and MAb 65) and M1, as indicated. WT, wild type; NC, negative control (i.e., transfected with only pCAXL-PA, pCAXL-PB1, pCAXL-PB2, and pCAXL-NP); ST, mutant with stop codons introduced after M2-Asp24; NT, nontransfected.

Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H., ... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.

Figure 2 Binding of MAb 65 to cells infected with influenza A virus.

Figure 2 Binding of MAb 65 to cells infected with influenza A virus.

Confocal images of immunofluorescence staining of MDCK cells infected with influenza virus. M2 staining is shown in red fluorescence and was revealed using MAb 65 or MAb 148 (a TCN-031-like antibody that binds to the N terminus of M2). Goat polyclonal antibody against influenza virus ribonucleoprotein (BEI Resources, no. NR-3133) was used to stain viral RNPs (green).

Cho, K. J., Schepens, B., Seok, J. H., Kim, S., Roose, K., Lee, J. H., ... & Fiers, W. (2015). Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody. Journal of virology, 89(7), 3700-3711.


Specifications

  • Immunogen
  • Recombinant 45 tetrameric protein of M2e
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native Influenza a virus M2e
  • Species Reactivity
  • Influenza a virus
  • Clone
  • MAb148
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • M2 protein; matrix protein 2; M2e

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Human Antibody

CAT Product Name Application Type
TAB-508CL Anti-Influenza Virus M2e Recombinant Antibody (TCN-032) FuncS Antibody

Chimeric Antibody

CAT Product Name Application Type
PABX-140-S (P) Recombinant Mouse Anti-M2e Antibody scFv Fragment (MAb148) WB, ELISA, FuncS scFv

Mouse Antibody

CAT Product Name Application Type
PABX-140-F (E) Recombinant Mouse Anti-M2e Antibody Fab Fragment (MAb148) WB, ELISA, FuncS Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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