Anti-MET immunotoxin (Fab)-PE (CAT#: AGTO-G049E)
This immunotoxin was achieved by conjugating/fusing the Anti-MET Fab to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to gastric cancer cells. Once bind, the toxin enters cells by receptor-mediated endocytosis. Then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treating gastric cancer.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55607/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55607/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/if_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18577/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18577/if_selected.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_7_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_6_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (A-431, CAPAN-2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105976-MET
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105976-MET
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Molecule Class
- Fab-PE
- Toxin Description
- Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- gastric cancer
Target
- Alternative Names
- MET; MET proto-oncogene, receptor tyrosine kinase; HGFR; AUTS9; RCCP2; c-Met; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE75
- Gene ID
- 4233
- UniProt ID
- A0A024R759
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MET"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-190-F(E) | Anti-Human Met(Onartuzumab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | Fab / Fc - G1 - kappa |
| TAB-0332CL-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-0332CL-F(E)) | ELISA | Humanized Fab |
| TAB-026MZ-F(E) | Anti-Human MET Recombinant Antibody Fab Fragment (73R009) | WB | Humanized antibody |
| TAB-0881CLV | Human Anti-MET Recombinant Antibody (TAB-0881CLV) | ELISA, FuncS | Humanized IgG1 |
| TAB-0883CLV | Human Anti-MET Recombinant Antibody (TAB-0883CLV) | ELISA, FuncS | Humanized IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-271 | Human Anti-MET Recombinant Antibody (clone 5D5); scFv Fragment | WB, Block, FuncS | Human scFv |
| HPAB-0123-FY-S(P) | Camelid Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0123-FY-S(P)) | ELISA | Camelid scFv |
| HPAB-0117-WJ-S(P) | Mouse Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0117-WJ-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-0486-FY-S(P) | Mouse Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0486-FY-S(P)) | Inhib | Mouse scFv |
| HPAB-0487-FY-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0487-FY-S(P)) | Inhib | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-155 | Afuco™ Anti-MET ADCC Recombinant Antibody (Onartuzumab), ADCC Enhanced | ELISA, Neut | ADCC enhanced antibody |
| AFC-TAB-H24 | Afuco™ Anti-MET ADCC Recombinant Antibody (Emibetuzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0530CL | Anti-Human MET Recombinant Antibody | ELISA, FC, IF, FuncS | |
| TAB-536LC | Human Anti-MET Recombinant Antibody (TAB-536LC) | Inhib | Human IgG2 |
| TAB-537LC | Human Anti-MET Recombinant Antibody (TAB-537LC) | Inhib | Human IgG2 |
| TAB-538LC | Human Anti-MET Recombinant Antibody (TAB-538LC) | Inhib | Human IgG2 |
| TAB-539LC | Human Anti-MET Recombinant Antibody (TAB-539LC) | Inhib | Human IgG2 |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002MZ | Anti-Human MET Recombinant Antibody (MvDN30) | IHC, PET imaging | Chimeric antibody (mouse/human) |
| TAB-002MZ-S(P) | Anti-Human MET Recombinant Antibody scFv Fragment (MvDN30) | IHC, PET imaging | Chimeric antibody (mouse/human) |
| TAB-0880CLV | Human Anti-MET Recombinant Antibody (TAB-0880CLV) | ELISA, FuncS | Chimeric (mouse/human) IgG1 |
| TAB-0880CLV-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-0880CLV-F(E)) | ELISA, FuncS | Chimeric (mouse/human) Fab |
| TAB-0880CLV-S(P) | Mouse Anti-MET Recombinant Antibody; scFv Fragment (TAB-0880CLV-S(P)) | ELISA, FuncS | Mouse scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-011MZ | Mouse Anti-MET Recombinant Antibody (TAB-011MZ) | ELISA, WB, FC | Mouse IgG |
| TAB-012MZ | Mouse Anti-MET Recombinant Antibody (TAB-012MZ) | ELISA, WB, FC | Mouse IgG |
| TAB-027MZ | Mouse Anti-MET Recombinant Antibody (TAB-027MZ) | WB, IP, IF | Mouse lgG1 |
| TAB-028MZ | Mouse Anti-MET Recombinant Antibody (TAB-028MZ) | IP | Mouse lgG1 |
| TAB-029MZ | Mouse Anti-MET Recombinant Antibody (TAB-029MZ) | IP, WB | Mouse lgG1 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1726CQ | Recombinant Rabbit Anti-MET Antibody (CBL1008) | Neut | IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2216 | Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (DS2216AB) | ICC/IF, IHC-P, WB | IgG |
| MOR-4519 | Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH27DS) | WB, IF, ICC | IgG |
| MOR-4645 | Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH158DS) | WB, IF, ICC, FC, ELISA | IgG |
| MOR-4694 | Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH208DS) | WB, ELISA | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0760-CN | Mouse Anti-MET Recombinant Antibody (HPAB-0760-CN) | WB, ELISA, IHC, IP | Mouse IgG |
| HPAB-0761-CN | Human Anti-MET Recombinant Antibody (HPAB-0761-CN) | WB, ELISA, IHC, IP | Chimeric (mouse/human) IgG1, κ |
| HPAB-0762-CN | Human Anti-MET Recombinant Antibody (HPAB-0762-CN) | WB, ELISA, IHC, IP | Humanized IgG1, κ |
| HPAB-1298LY | Human Anti-MET Recombinant Antibody (HPAB-1298LY) | ELISA, IP | Human IgG2 |
| HPAB-1299LY | Human Anti-MET Recombinant Antibody (HPAB-1299LY) | ELISA, IP | Human IgG2 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1298LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1298LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1299LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1299LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1300LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1300LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1301LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1301LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1302LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1302LY-F(E)) | ELISA, IP | Human Fab |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF474 | A*02:01/Human Hepatocyte growth factor receptor (YVDPVITSI) MHC Pentamer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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