Recombinant Mouse Anti-MK2-23 idiotype Antibody scFv Fragment (GH1002) (CAT#: PABX-142-S (P))

Anti-MK2-23 idiotype antibody is a Mouse of scFv class that binds to an MK2-23 idiotype. The antibody binds specifically to MK2-23, an idiotypic monoclonal antibody known to induce immunity in patients with malignant melanoma.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Packing of the anti-anti-id Fab fragment of mAb GH1002 in tetragonal unit cell viewed in stereo approximately down the z axis of the coordinate system.

Figure 1 Packing of the anti-anti-id Fab fragment of mAb GH1002 in tetragonal unit cell viewed in stereo approximately down the z axis of the coordinate system.

There are eight asymmetric units in this unit cell, each formed by one Fab. The two 2-fold crystallographically related Fab fragments forming a complex are colored in red, green, blue and yellow, and the coordinate axes are in red. The Figure was generated with ''O'' (Jones & Kjeldgaard, 1990), and displayed on an SGI 320 VGX workstation.

Ban, N., Day, J., Wang, X., Ferrone, S., & McPherson, A. (1996). Crystal structure of an anti-anti-idiotype shows it to be self-complementary. Journal of molecular biology, 255(4), 617-627.

Figure 2 Particle size distribution determined using light scattering.

Figure 2 Particle size distribution determined using light scattering.

Two different measurements are combined in this Figure. The distribution indicated by a broken line was obtained by measuring a solution containing a Fab fragment of an anti-anti-idiotopic mAb GH1002; as a control, the continuous line represents the distribution of mass in a solution containing a Fab fragment of idiotopic mAb 763-74. The average hydrodynamic radius of particles is 69A° and 51A°, respectively, corresponding to a dimer formed by a head-to-head interaction of two Fab fragments and a monomer. The
measurement was performed for a duration of 60 seconds at 295 K.

Ban, N., Day, J., Wang, X., Ferrone, S., & McPherson, A. (1996). Crystal structure of an anti-anti-idiotype shows it to be self-complementary. Journal of molecular biology, 255(4), 617-627.

Figure 3 Powell minimized Fo - Fc omit-map for CDRs of anti-anti-id mAb GH1002.

Figure 3 Powell minimized Fo - Fc omit-map for CDRs of anti-anti-id mAb GH1002.

The model bias was minimized by refining the model lacking CDRs and subsequently calculating the map. CDR loops are stick model in white, the rest of the molecule is shown as a Ca trace, and symmetry-related atoms are in red. The Figure was generated with ''O'' (Jones & Kjeldgaard, 1990), and displayed on an SGI 320 VGX workstation.

Ban, N., Day, J., Wang, X., Ferrone, S., & McPherson, A. (1996). Crystal structure of an anti-anti-idiotype shows it to be self-complementary. Journal of molecular biology, 255(4), 617-627.


Specifications

  • Immunogen
  • mAb MK2-23
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • scFv
  • Specificity
  • Tested positive against native BVDV
  • Species Reactivity
  • Mouse
  • Clone
  • GH1002
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone GH1002"

See other products for "MK2-23 idiotype"

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for PABX-142-S (P). Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare