Recombinant Human Antibody (E2) is capable of binding to MT-SP1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-MT-SP1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-MT-SP1 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 Inhibitors displace pAB from the MT-SP1 active site.
pAB (270 μM) incubated with 1 μM MT-SP1 emits a strong emission peak with a maximum at 361 nm when excited at 325 nm, due to hydrophobic interactions between pAB and the P1 pocket of the protease. When one equivalent of either S4 (blue trace) or E2 (green trace) is added to 1 μM MT-SP1 saturated with pAB, the fluorescence decreases, suggesting pAB is released into the aqueous environment, where it is weakly fluorescent. Therefore, binding of both S4 and E2 are competitive with pAB binding, and both inhibitors bind in or near the P1 pocket in a manner that precludes binding of pAB.
Farady, C. J., Sun, J., Darragh, M. R., Miller, S. M., & Craik, C. S. (2007). The mechanism of inhibition of antibody-based inhibitors of membrane-type serine protease 1 (MT-SP1). Journal of molecular biology, 369(4), 1041-1051.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-679 | Recombinant Anti-MT-SP1 Antibody | ELISA, WB, FC, FuncS | IgG |
MHH-679 | Recombinant Human Anti-MT-SP1 Antibody | FC, IP, FuncS | IgG |
PABL-660 | Human Anti-MT-SP1 Recombinant Antibody (clone S4) | ELISA, BL | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-679-F(E) | Recombinant Anti-MT-SP1 Antibody Fab Fragment | IF, FuncS | Fab |
MHH-679-F(E) | Recombinant Human Anti-MT-SP1 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
PFBZ-090 | Human Anti-MT-SP1 Recombinant Antibody (clone E2); Fab Fragment | Block | Human Fab |
PFBL-654 | Human Anti-MT-SP1 Recombinant Antibody (clone S4); Fab Fragment | ELISA, BL | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-679-S(P) | Recombinant Anti-MT-SP1 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
MHH-679-S(P) | Recombinant Human Anti-MT-SP1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
PSBZ-090 | Human Anti-MT-SP1 Recombinant Antibody (clone E2); scFv Fragment | Block | Human scFv |
PSBL-654 | Human Anti-MT-SP1 Recombinant Antibody (clone S4); scFv Fragment | ELISA, BL | Human scFv |
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