Provided here is a humanized antibody that recognizes human MUC-1. This antibody internalization from the cell surface occurs in an efficient temperature-dependent manner. It also exerts specific cytotoxicity against cancer cells.
Figure 1 Immunoreactivity of DMB mAbs with MUC1-Xex.
ELISA plates were coated with 1 of 3 recombinant bacterial MUC1 proteins extracellular domain of the MUC1-X protein (dotted lines), MUC1 SEA module (dashed lines), or the uncleaved MUC1 4G-SEA module protein (dashed and dotted lines). The indicated mAbs were applied and bound antibody was detected as described in Materials and Methods.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
Figure 2 Titration analysis of DMB monoclonal anti-MUC1 SEA module α/β junction antibodies to human breast cancer cells expressing MUC1 and affinity analysis.
Antibody DMB5F3 was shown to have the highest affinity.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
Figure 3 Flow cytometry analyses were carried out with the chDMB5F3 antibody at the indicated concentrations using MUC1-negative parental DA3 cells (DA3-PAR) and mouse cell transfectants (DA3-TM) expressing human MUC1-TM (E and F, respectively).
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
Figure 4 MUC1+ cells were incubated with DyLight649-labeled DMB5F3 mAb at 4°C and at 37°C (G and H, respectively) and photographed by confocal laser microscopy.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
Figure 5 Specificity and affinity of chDMB5F3 immunotoxin (IT) for cell killing of cancer cells.
Pichinuk, E., Benhar, I., Jacobi, O., Chalik, M., Weiss, L., Ziv, R., ... & Wreschner, D. H. (2012). Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer research, 72(13), 3324-3336.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
NAB-2049-VHH | Recombinant Anti-human MUC1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
HPAB-AP504-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (5-24) | FC | Camel VHH |
HPAB-AP505-YC | Recombinant Camel Anti-MUC1 Single Domain Antibody (aMUC1) | ELISA | Camel VHH |
HPAB-0736-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (ER46) | ELISA | Camelid VHH |
HPAB-0737-YJ-VHH | Camelid Anti-MUC1 Recombinant Single Domain Antibody (AR-32) | ELISA | Camelid VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-173 | Anti-Human MUC1 Recombinant Antibody (Cantuzumab Ravtansine) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
TAB-166 | Humanized Anti-MUC1 Recombinant Antibody (clone Cantuzumab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | Humanized (from mouse) IgG1, κ |
TAB-413MZ | Human Anti-MUC1 Recombinant Antibody (TAB-413MZ) | ELISA | Humanized IgG |
TAB-430MZ | Anti-Human MUC1 Recombinant Antibody (BLC595) | ELISA, FC | Humanized antibody |
TAB-026ML | Anti-Human MUC1 Recombinant Antibody (Gatipotuzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H56 | Anti-Human MUC1 Recombinant Antibody (Pemtumomab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG |
TAB-0388CL-S(P) | Mouse Anti-Human MUC1 Recombinant Antibody scFv Fragment | WB, IHC | |
TAB-411MZ | Mouse Anti-MUC1 Recombinant Antibody (TAB-411MZ) | ELISA | Mouse IgG |
TAB-439MZ-S(P) | Anti-Human MUC1 Recombinant Antibody scFv Fragment (1B2) | FC | |
PABX-183-S (P) | Recombinant Mouse Anti-MUC1 Antibody scFv Fragment (CTM01) | Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-L036E | anti-MUC1 immunotoxin C242 (Fab)-PE | Cytotoxicity assay, Functional assay | |
AGTO-L064E | anti-MUC1 immunotoxin H23 (scFv)-PE | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-656 | Mouse Anti-MUC1 Recombinant Antibody; Fab Fragment | WB, IF, FuncS | Mouse Fab |
FAMAB-0225-YC-F(E) | Mouse Anti-MUC1 Recombinant Antibody (clone MUSE11); Fab Fragment | IHC | Mouse Fab |
HPAB-0670-CN-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0670-CN-F(E)) | FC | Human Fab |
HPAB-0495-FY-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0495-FY-F(E)) | ELISA | Human Fab |
HPAB-0496-FY-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (HPAB-0496-FY-F(E)) | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-412MZ | Human Anti-MUC1 Recombinant Antibody (TAB-412MZ) | ELISA | Chimeric (mouse/human) IgG |
TAB-412MZ-S(P) | Mouse Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-412MZ-S(P)) | ELISA | Mouse scFv |
TAB-412MZ-F(E) | Human Anti-MUC1 Recombinant Antibody; Fab Fragment (TAB-412MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-431MZ-S(P) | Anti-Human MUC1 Recombinant Antibody scFv Fragment (PH1) | ELISA, WB, FC, IHC | Human antibody |
TAB-432MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-432MZ-S(P)) | ELISA, FC | Human scFv |
TAB-433MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-433MZ-S(P)) | ELISA, FC | Human scFv |
TAB-434MZ-S(P) | Human Anti-MUC1 Recombinant Antibody; scFv Fragment (TAB-434MZ-S(P)) | ELISA, FC | Human scFv |
PABX-143 | Recombinant Human Anti-MUC1 Antibody (CTM01) | WB, ELISA, FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-012LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
Gly-120LC | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-012LC-1 | Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) | ELISA, FC, IHC | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-109LC | Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-121LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Gly-122LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Gly-123LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
Gly-124LC | Recombinant Anti-Human MUC1 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0379MZ | Chicken Anti-MUC1 Polyclonal IgY | IHC, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC054 | PE-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
MHC-LC055 | APC-A*02:01/Human MUC1 (LLLTVLTVV) MHC Tetramer | FCM | |
MHC-LC1950 | PE-A2/Human MUC1 (NLTISDVSV) MHC Tetramer | FCM | |
MHC-YF272 | A*02:01/Human MUC1 (LLLLTVLTV) MHC Monomer | MHC Multimer | |
MHC-YF570 | A*02:01/Human Mucin (TLAPATEPA) MHC Pentamer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2320 | Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) | IHC-P, IHC-Fr | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0838LC | Recombinant Mouse Anti-Human MUC1 Antibody (SM3) | ELISA | IgG1 |
EPAF-0581CQ | Recombinant Mouse Anti-Human MUC1 Antibody (B27.29) | Neut, FC | IgG1 |
EPAF-0583CQ | Recombinant Mouse Anti-Human MUC1 Antibody (BC4E549) | Neut, FC | IgG1 |
EPAF-0584CQ | Recombinant Mouse Anti-Human MUC1 Antibody (DF3) | Neut, FC | IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0091-YC | Mouse Anti-MUC1 Recombinant Antibody (HPAB-0091-YC) | ELISA, Neut | Mouse IgG |
HPAB-0094-YC | Mouse Anti-MUC1 Recombinant Antibody (clone 12D10) | ELISA, FC | Mouse IgG2a |
FAMAB-0225YC | Mouse Anti-MUC1 Recombinant Antibody (clone MUSE11) | IHC | Mouse IgG1 |
MRO-1039-CN | Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (CBACN-385) | WB, IF, IHC, IP, FC | Rabbit IgG |
HPAB-0495-FY | Human Anti-MUC1 Recombinant Antibody (HPAB-0495-FY) | ELISA, WB, IP | Human IgE |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBC-399 | Mouse Anti-MUC1 Recombinant Antibody (clone AR20.5); scFv Fragment | ELISA, FC, FuncS | Mouse scFv |
PSBC-400 | Mouse Anti-MUC1 Recombinant Antibody (clone SM3); scFv Fragment | FC, IHC-Fr, IF, ELISA, IHC-P | Mouse scFv |
HPAB-J0212-YC-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone BW835); scFv Fragment | ELISA, IHC | Mouse scFv |
HPAB-1159WJ-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone 12E); scFv Fragment | ELISA, ICC | Mouse scFv |
HPAB-1160WJ-S(P) | Mouse Anti-MUC1 Recombinant Antibody (clone 3D); scFv Fragment | ELISA, ICC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H56 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Pemtumomab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-H65 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Sontuzumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-026ML | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Gatipotuzumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-166 | Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Cantuzumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
AFC-TAB-H77 | Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) | ELISA, FC, IP, FuncS, IF | IgG1, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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