This product was developed by immunization of mice with human ovarian cancer cell line OVCAR-3 and then was produced as a recombinant antibody. It identifies the epitope in the CA125 protein.
Figure 1 Immunofluorescence micrographs demonstrating the presence of the membrane-associated mucin MUC16 in corneal and conjunctival epithelial cells.
Binding of the monoclonal antibody OC125 (A) or VK-8 (B) or the polyclonal antibody R16 (C) raised against the MUC16 mucin was observed in apical cells of the corneal epithelium. There was no background binding associated with the secondary antibodies, anti-mouse IgG (D), and anti-rabbit IgG (E). Binding of the OC125 antibody to the suprabasal cells of the conjunctival epithelium is demonstrated in (F). Bottom inset: Intracellular binding of the OC125 antibody was observed in some goblet cells. Top inset: Phase-contrast image of the section in the bottom inset demonstrating that the cell shown in the bottom inset is a goblet cell. Binding of the OC125 antibody to scattered islands of cells in HCLE cultures is shown in (G). Propidium iodide was included in the mounting medium to localize the position of the nuclei of cells in culture (G, arrows). Dashed lines: basement membrane region of the epithelia. Scale bar, 25 μm.
Argüeso, P., Spurr-Michaud, S., Russo, C. L., Tisdale, A., & Gipson, I. K. (2003). MUC16 mucin is expressed by the human ocular surface epithelia and carries the H185 carbohydrate epitope. Investigative ophthalmology & visual science, 44(6), 2487-2495.
Figure 2 OC125 weakly binds to cells that are generally considered negative for MUC16.
SKOV-3 cells (identity confirmed by STR analysis) were stained with the primary (1°) antibodies OC125 or VK8 followed by incubation with allophycocyanine (APC)-labeled donkey anti-mouse (DAM) secondary antibody. Binding of the antibodies to SKOV-3 cells was determined on a LSR-II flow cytometer. RT-PCR using MUC16 primers previously reported, was used to determine expression of MUC16 in OVCAR-3, ECC-1 and SKOV-3 cells.
Felder, M., Kapur, A., Gonzalez-Bosquet, J., Horibata, S., Heintz, J., Albrecht, R.,... & Whelan, R. J. (2014). MUC16 (CA125): tumor biomarker to cancer therapy, a work in progress. Molecular cancer, 13(1), 129.
Figure 3 Cell surface MUC16 is sensitive to proteolysis.
A, Binding of OC125 and VK8 to OVCAR-3 cells that were harvested with trypsin or EDTA containing media was determined by flow cytometry. A significant decrease in VK8 binding to cells harvested after trypsin treatment was observed whereas under these conditions, OC125 binding was less affected.
Felder, M., Kapur, A., Gonzalez-Bosquet, J., Horibata, S., Heintz, J., Albrecht, R.,... & Whelan, R. J. (2014). MUC16 (CA125): tumor biomarker to cancer therapy, a work in progress. Molecular cancer, 13(1), 129.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H63 | Humanized Anti-MUC16 Recombinant V-kappa Antibody (clone Sofituzumab) | IF, IP, Neut, FuncS, ELISA, FC, WB | Humanized IgG1, κ |
TAB-1397CL-S(P) | Human Anti-MUC16 Recombinant Antibody; scFv Fragment (TAB-1397CL-S(P)) | FuncS | Humanized scFv |
TAB-1399CL-S(P) | Human Anti-MUC16 Recombinant Antibody; scFv Fragment (TAB-1399CL-S(P)) | FuncS | Humanized scFv |
TAB-0339CL-F(E) | Human Anti-MUC16 Recombinant Antibody; Fab Fragment (TAB-0339CL-F(E)) | DB, WB, IP, ELISA, FC | Humanized Fab |
TAB-1397CL-F(E) | Human Anti-MUC16 Recombinant Antibody; Fab Fragment (TAB-1397CL-F(E)) | FuncS | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-114 | Anti-Human CA-125 Recombinant Antibody (Abagovomab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | IgG1 - kappa |
TAB-1401CL-S(P) | Anti-Human MUC16 Recombinant Antibody scFv Fragment (OC125) | IF, IHC, IP, FC | |
TAB-1402CL-S(P) | Anti-Human MUC16 Recombinant Antibody scFv Fragment (VK-8) | IF, FC | |
TAB-1401CL-F(E) | Anti-Human MUC16 Recombinant Antibody Fab Fragment (OC125) | IF, IHC, IP, FC | |
TAB-1402CL-F(E) | Anti-Human MUC16 Recombinant Antibody Fab Fragment (VK-8) | IF, FC |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1398CL | Human Anti-MUC16 Recombinant Antibody (TAB-1398CL) | FuncS | Chimeric (mouse/human) IgG |
TAB-1400CL | Human Anti-MUC16 Recombinant Antibody (TAB-1400CL) | FuncS | Chimeric (mouse/human) IgG |
TAB-1398CL-S(P) | Mouse Anti-MUC16 Recombinant Antibody; scFv Fragment (TAB-1398CL-S(P)) | FuncS | Mouse scFv |
TAB-1398CL-F(E) | Human Anti-MUC16 Recombinant Antibody; Fab Fragment (TAB-1398CL-F(E)) | FuncS | Chimeric (mouse/human) Fab |
TAB-1400CL-F(E) | Human Anti-MUC16 Recombinant Antibody; Fab Fragment (TAB-1400CL-F(E)) | FuncS | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2321 | Hi-Affi™ Recombinant Rabbit Anti-MUC16 Monoclonal Antibody (DS2321AB) | ICC, IHC-P, WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0043-YC-S(P) | Mouse Anti-MUC16 Recombinant Antibody (clone 196-14); scFv Fragment | FuncS | Mouse scFv |
FAMAB-0044-YC-S(P) | Mouse Anti-MUC16 Recombinant Antibody (clone ch196-14); scFv Fragment | FuncS | Mouse scFv |
HPAB-0120LY-S(P) | Mouse Anti-MUC16 Recombinant Antibody; scFv Fragment (HPAB-0120LY-S(P)) | WB, ELISA, FC, IHC | Mouse scfv |
HPAB-0121LY-S(P) | Mouse Anti-MUC16 Recombinant Antibody; scFv Fragment (HPAB-0121LY-S(P)) | WB, ELISA, FC, IHC | Mouse scfv |
HPAB-0122LY-S(P) | Mouse Anti-MUC16 Recombinant Antibody; scFv Fragment (HPAB-0122LY-S(P)) | WB, ELISA, FC, IHC | Mouse scfv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0043-YC-F(E) | Mouse Anti-MUC16 Recombinant Antibody (clone 196-14); Fab Fragment | FuncS | Mouse Fab |
FAMAB-0044-YC-F(E) | Human Anti-MUC16 Recombinant Antibody (clone ch196-14); Fab Fragment | FuncS | Human Fab |
HPAB-1114LY-F(E) | Mouse Anti-MUC16 Recombinant Antibody; Fab Fragment (HPAB-1114LY-F(E)) | ELISA | Mouse Fab |
HPAB-1115LY-F(E) | Mouse Anti-MUC16 Recombinant Antibody; Fab Fragment (HPAB-1115LY-F(E)) | ELISA | Mouse Fab |
HPAB-1116LY-F(E) | Mouse Anti-MUC16 Recombinant Antibody; Fab Fragment (HPAB-1116LY-F(E)) | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-114 | Afuco™ Anti-MUC16 ADCC Recombinant Antibody (Abagovomab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
AFC-TAB-H63 | Afuco™ Anti-MUC16 ADCC Recombinant Antibody (Sofituzumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0185-YC | Human Anti-MUC16 Recombinant Antibody (HPAB-N0185-YC) | FuncS | Humanized IgG |
ZG-0475U | Rabbit Anti-MUC16 Recombinant Antibody (clone 10E9) | ELISA, IHC | Rabbit IgG |
ZG-0476U | Rabbit Anti-MUC16 Recombinant Antibody (clone 10F10) | ELISA, IHC | Rabbit IgG |
VS3-FY977 | Recombinant Mouse Anti-MUC16 Antibody (clone 2E9C2) | ELISA, IHC, FC | Mouse IgG1 |
VS3-FY978 | Recombinant Mouse Anti-MUC16 Antibody (clone 4C8C8) | ELISA, IHC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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