Anti-Human MUC16 Recombinant Antibody (VK-8) (CAT#: TAB-1402CL)

This product was developed by immunization of mice with human ovarian cancer cell line OVCAR-3 and then was produced as a recombinant antibody. It identifies the epitope in the CA125 protein.


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IF

Figure 1 Immunofluorescence micrographs demonstrating the presence of the membrane-associated mucin MUC16 in corneal and conjunctival epithelial cells.

Figure 1 Immunofluorescence micrographs demonstrating the presence of the membrane-associated mucin MUC16 in corneal and conjunctival epithelial cells.

Binding of the monoclonal antibody OC125 (A) or VK-8 (B) or the polyclonal antibody R16 (C) raised against the MUC16 mucin was observed in apical cells of the corneal epithelium. There was no background binding associated with the secondary antibodies, anti-mouse IgG (D), and anti-rabbit IgG (E). Binding of the OC125 antibody to the suprabasal cells of the conjunctival epithelium is demonstrated in (F). Bottom inset: Intracellular binding of the OC125 antibody was observed in some goblet cells. Top inset: Phase-contrast image of the section in the bottom inset demonstrating that the cell shown in the bottom inset is a goblet cell. Binding of the OC125 antibody to scattered islands of cells in HCLE cultures is shown in (G). Propidium iodide was included in the mounting medium to localize the position of the nuclei of cells in culture (G, arrows). Dashed lines: basement membrane region of the epithelia. Scale bar, 25 μm.

Argüeso, P., Spurr-Michaud, S., Russo, C. L., Tisdale, A., & Gipson, I. K. (2003). MUC16 mucin is expressed by the human ocular surface epithelia and carries the H185 carbohydrate epitope. Investigative ophthalmology & visual science, 44(6), 2487-2495.

FC

Figure 2 OC125 weakly binds to cells that are generally considered negative for MUC16.

Figure 2 OC125 weakly binds to cells that are generally considered negative for MUC16.

SKOV-3 cells (identity confirmed by STR analysis) were stained with the primary (1°) antibodies OC125 or VK8 followed by incubation with allophycocyanine (APC)-labeled donkey anti-mouse (DAM) secondary antibody. Binding of the antibodies to SKOV-3 cells was determined on a LSR-II flow cytometer. RT-PCR using MUC16 primers previously reported, was used to determine expression of MUC16 in OVCAR-3, ECC-1 and SKOV-3 cells.

Felder, M., Kapur, A., Gonzalez-Bosquet, J., Horibata, S., Heintz, J., Albrecht, R.,... & Whelan, R. J. (2014). MUC16 (CA125): tumor biomarker to cancer therapy, a work in progress. Molecular cancer, 13(1), 129.

FC

Figure 3 Cell surface MUC16 is sensitive to proteolysis.

Figure 3 Cell surface MUC16 is sensitive to proteolysis.

A, Binding of OC125 and VK8 to OVCAR-3 cells that were harvested with trypsin or EDTA containing media was determined by flow cytometry. A significant decrease in VK8 binding to cells harvested after trypsin treatment was observed whereas under these conditions, OC125 binding was less affected.

Felder, M., Kapur, A., Gonzalez-Bosquet, J., Horibata, S., Heintz, J., Albrecht, R.,... & Whelan, R. J. (2014). MUC16 (CA125): tumor biomarker to cancer therapy, a work in progress. Molecular cancer, 13(1), 129.


Specifications

  • Immunogen
  • Human ovarian cancer cell line OVCAR-3
  • Host Species
  • Mouse
  • Specificity
  • Human
  • Clone
  • VK-8
  • Applications
  • IF, FC

Applications

  • Application Notes
  • Immunohistochemistry
    Immunohistochemistry with H185 (1:10 dilution), OC125 (1:50), R16 (1:100), or VK-8 (1:10) used as primary antibodies. To determine whether H185 antigen colocalizes with MUC16, double-labeling studies were conducted. A mixture of H185 antibody and R16 antibody was applied to the sections, followed by a mixture of TRITC-conjugated donkey anti-mouse IgG and FITC-conjugated donkey anti-rabbit IgG. Coverslips were applied to the slides using antifade mounting medium with DAPI (Vectashield; Vector Laboratories) to visualize the nuclei of the cells. Incubation with the primary antibody was omitted in control experiments.

Target

  • Alternative Names
  • MUC16; mucin 16, cell surface associated; CA125; mucin-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone VK-8"

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Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2321 Hi-Affi™ Recombinant Rabbit Anti-MUC16 Monoclonal Antibody (DS2321AB) ICC, IHC-P, WB IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-114 Afuco™ Anti-MUC16 ADCC Recombinant Antibody (Abagovomab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody
AFC-TAB-H63 Afuco™ Anti-MUC16 ADCC Recombinant Antibody (Sofituzumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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