Mouse Anti-MYC Recombinant Antibody (clone 9E10) (CAT#: PABL-281)

Figure 1 MYC expression in normal human colon.

Representative haematoxylin and eosin staining, in‐situ hybridization (MYCmRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminal MYC) and Ki67 antibodies (brown) in normal human colon. The lower panels focus on the surface epithelium, which appears negative for Y69 and Ki67 expression, but strongly positive for 9E10 expression. Scale bars: 100 μm (upper panels) and 50 μm (lower panels).

Baker, A. M., Van Noorden, S., Rodriguez‐Justo, M., Cohen, P., Wright, N. A., & Lampert, I. A. (2016). Distribution of the c‐MYC gene product in colorectal neoplasia. Histopathology,69(2), 222-229.

Figure 2 MYC expression in conventional adenomas.

A, Representative haematoxylin and eosin (H&E) staining, in‐situhybridization (MYC mRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminalMYC) and Ki67 antibodies (brown) in adenoma with low‐grade dysplasia. A 'reverse gradient' of MYC mRNA and Y69 expression, with highest expression at the luminal surface, is apparent. Scale bars: 200 μm. B, Representative H&E staining, in‐situ hybridization (MYC mRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminal MYC) and Ki67 antibodies (brown) in adenoma, focusing on a gland with a highly proliferative phenotype. Again, a reverse gradient is apparent, although, in the proliferative gland, the MYC mRNA, Y69 and Ki67 expression can be seen extending to the base of the crypt, in contrast to the surrounding glands and normal crypts. Scale bars: 500 μm (upper panels) and 200 μm (lower panels). C, Representative H&E staining, in‐situ hybridization (MYC mRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminal MYC) and Ki67 antibodies (brown) in high‐grade dysplasia. Extensive positivity for MYC mRNA, Y69 and Ki67 is apparent, whereas 9E10 staining is remarkably weak. Scale bars: 200 μm.

Baker, A. M., Van Noorden, S., Rodriguez‐Justo, M., Cohen, P., Wright, N. A., & Lampert, I. A. (2016). Distribution of the c‐MYC gene product in colorectal neoplasia. Histopathology,69(2), 222-229.

Figure 3 MYC expression in traditional serrated adenomas.

Representative haematoxylin and eosin staining, in‐situhybridization (MYC mRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminalMYC) and Ki67 antibodies (brown) in a traditional serrated adenoma. Strong positivity for MYC mRNA, Y69 and Ki67 is apparent in ectopic crypt foci. Scale bars: 200 μm (upper panels) and 100 μm (lower panels).

Baker, A. M., Van Noorden, S., Rodriguez‐Justo, M., Cohen, P., Wright, N. A., & Lampert, I. A. (2016). Distribution of the c‐MYC gene product in colorectal neoplasia. Histopathology,69(2), 222-229.

Figure 4 MYC expression in hyperplastic polyps.

Representative haematoxylin and eosin staining, in‐situ hybridization (MYCmRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminal MYC) and Ki67 antibodies (brown) in a hyperplastic polyp. The lower panels focus on the surface epithelium, which appears negative for Y69 and Ki67 expression, but strongly positive for 9E10 expression. Scale bars: 500 μm (upper panels) and 100 μm (lower panels).

Baker, A. M., Van Noorden, S., Rodriguez‐Justo, M., Cohen, P., Wright, N. A., & Lampert, I. A. (2016). Distribution of the c‐MYC gene product in colorectal neoplasia. Histopathology,69(2), 222-229.

Figure 5 MYC expression in invasive carcinoma.

A, Representative haematoxylin and eosin (H&E) staining, in‐situhybridization (MYC mRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminalMYC) and Ki67 antibodies (brown) in a region of invasive carcinoma. The lower panels highlight the reciprocal relationship between the expression of MYC mRNA, Y69, and Ki67, and the staining of the 9E10 antibody. B, Representative H&E staining, in‐situ hybridization (MYC mRNA, pink) and immunohistochemical staining with Y69 (N‐terminal MYC), 9E10 (C‐terminal MYC) and Ki67 antibodies (brown) in a region of invasive carcinoma. The lower panels focus on an invasive gland showing flattened non‐proliferative cells at the invasive edge, which are notably Y69‐negative and 9E10‐positive. Scale bars: 200 μm (upper panels) and 50 μm (lower panels).

Baker, A. M., Van Noorden, S., Rodriguez‐Justo, M., Cohen, P., Wright, N. A., & Lampert, I. A. (2016). Distribution of the c‐MYC gene product in colorectal neoplasia. Histopathology,69(2), 222-229.

Figure 6 Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining.

Figure 7 Western blot analysis of c-Myc expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates.