Recombinant Anti-Methotrexate (MTX) VHH Single Domain Antibody (R3.40) (CAT#: PNBL-091)
Anti-Methotrexate (MTX) VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Specific Inquiry
ELISA
Figure 1 Competition ELISA.
Representative curves showing the inhibition of VHH binding to MTX–BSA of R3.25, R3.03, R3.40, R3.21, R3.27, R3.12, R3.04 (*) by increasing amounts of free MTX. MTX–BSA was immobilized on microtiter plates, and competition mixtures were then added to replicate wells.
Alvarez-Rueda, N., Behar, G., Ferré, V., Pugniere, M., Roquet, F., Gastinel, L., ... & Birklé, S. (2007). Generation of llama single-domain antibodies against methotrexate, a prototypical hapten. Molecular immunology, 44(7), 1680-1690.
Specifications
- Host Species
- Llama
- Derivation
- Llama
- Type
- Llama VHH
- Clone
- R3.40
- Applications
- ELISA, WB
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for ELISA, Western Blot. For details, refer to Published Data.
Target
- Alternative Names
- METHOTREXATE; METHYLAMINOPTERIN; METHYLAMINOPTERINE; ANTIFOLAN; L-(+)-AMETHOPTERIN; 4-AMINO-N 10-METHYLPTEROYL-L-GLUTAMIC ACID; 4-AMINO-10-METHYLPTEROYLGLUTAMIC ACID; (+)-4-AMINO-10-METHYLFOLIC ACID
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MTX"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-680-F(E) | Recombinant Anti-MTX Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-680-F(E) | Recombinant Human Anti-MTX Antibody Fab Fragment | IP, Neut, FuncS | Fab |
| PFBL-273 | Mouse Anti-MTX Recombinant Antibody (clone ADD056); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| HPAB-0725-FY-S(P) | Mouse Anti-MTX Recombinant Antibody (clone AB119); Fab Fragment | ELISA | Mouse Fab |
| HPAB-AP396-YC-F(E) | Mouse Anti-MTX Recombinant Antibody; Fab Fragment (HPAB-AP396-YC-F(E)) | ECL | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-680-S(P) | Recombinant Anti-MTX Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| HPAB-0725-FY-F(E) | Mouse Anti-MTX Recombinant Antibody (clone AB119); scFv Fragment | ELISA | Mouse scFv |
| HPAB-AP396-YC-S(P) | Mouse Anti-MTX Recombinant Antibody; scFv Fragment (HPAB-AP396-YC-S(P)) | ECL | Mouse scFv |
| HPAB-AP397-YC-S(P) | Mouse Anti-MTX Recombinant Antibody; scFv Fragment (HPAB-AP397-YC-S(P)) | ECL | Mouse scFv |
| HPAB-AP398-YC-S(P) | Mouse Anti-MTX Recombinant Antibody; scFv Fragment (HPAB-AP398-YC-S(P)) | ECL | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-273 | Mouse Anti-MTX Recombinant Antibody (clone ADD056) | WB, ELISA, FuncS | Mouse IgG |
| HPAB-0725-FY | Mouse Anti-MTX Recombinant Antibody (clone AB119) | ELISA | Mouse IgG |
| HPAB-AP396-YC | Mouse Anti-MTX Recombinant Antibody (HPAB-AP396-YC) | ECL | Mouse IgG |
| HPAB-AP397-YC | Mouse Anti-MTX Recombinant Antibody (HPAB-AP397-YC) | ECL | Mouse IgG |
| HPAB-AP398-YC | Mouse Anti-MTX Recombinant Antibody (HPAB-AP398-YC) | ECL | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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