Anti-Mouse Abcb1a VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Figure 1 Nb592 is a strong inhibitor of P-gp’s ATPase activity.
Nb592 inhibits verapamil-stimulated ATPase activity with an IC50 of 520 ± 57 nM. Data points indicate the average activity ± SEM from four independent experiments, relative to P-gp's activity in the absence of Nb592.
Ward, A. B., Szewczyk, P., Grimard, V., Lee, C. W., Martinez, L., Doshi, R., ... & Swartz, D. J. (2013). Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Proceedings of the National Academy of Sciences, 110(33), 13386-13391.
Figure 2 Nb592 is a strong inhibitor of P-gp’s ATPase activity.
Inhibition of verapamil-stimulated ATPase activity in the presence of 5 mM DTT, without or with 7.5 μM Nb592 (n = 4).
Ward, A. B., Szewczyk, P., Grimard, V., Lee, C. W., Martinez, L., Doshi, R., ... & Swartz, D. J. (2013). Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Proceedings of the National Academy of Sciences, 110(33), 13386-13391.
Figure 3 Nb592 is a strong inhibitor of P-gp’s ATPase activity.
Nb592 prevents vanadate-induced 8-azido-[α³²P]-ADP trapping in P-gp's catalytic sites. P-gp was incubated with 100 μM verapamil (VER) and 200 μM orthovanadate (Vi) in the 8-azido-[α³²P]-ATP hydrolysis/trapping reaction as indicated above the lanes.
Ward, A. B., Szewczyk, P., Grimard, V., Lee, C. W., Martinez, L., Doshi, R., ... & Swartz, D. J. (2013). Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Proceedings of the National Academy of Sciences, 110(33), 13386-13391.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
There are currently no Customer reviews or questions for PNBL-079. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.