Rabbit Anti-AGER Recombinant Antibody (clone H4) (CAT#: PABZ-175)

Figure 4 Surface plasmon resonance runs showing the interaction of the recombinant ectoplasmic region of AMA1 as analyte and IgG F8.12.19 covalently immobilised on the Biacore sensor chip.

The runs were corrected for non-specific binding by subtraction of the curves obtained by passage of the same protein solution through a blank channel (no immobilised IgG) on the same senor chip. The curves labelled buffer show the results for passage of the running buffer alone. Analyte was injected for 60 s for the association phase. This was followed by injection of the running buffer alone at the same flow-rate to give the dissociation phase. The response in resonance units (RU) is plotted as a function of time (in s.). PvAMA1 at concentrations 5.0 μg/ml, 2.5 μg/ml, 1.25 μg/ml and 0.62 μg/ml, as indicated.

Igonet, S., Vulliez-Le Normand, B., Faure, G., Riottot, M. M., Kocken, C. H., Thomas, A. W., & Bentley, G. A. (2007). Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation. Journal of molecular biology, 366(5), 1523-1537.