Rat Anti-CD8A Recombinant Antibody (clone YTS 105.18) (CAT#: PABZ-144)
Recombinant Rat Antibody (YTS 105.18) is capable of binding to Mouse CD8a, expressed in Chinese Hamster Ovary cells (CHO). Analysis of the YTS 105.18 Fab epitope on CD8alpha reveals that this antibody blocks CD8 activity by hydrogen bonding to residues that are critical for interaction with both class I pMHC and TL.
Specific Inquiry
Figure 1 The effect of injection of rat IgG2, anti-CD4 and anti-CD8 mAb on the peripheral blood Tcells.
Normal CBA/Ca mice (n = 4) were given injections of the rat IgG2a, anti-CD4 mAb (YTS 177.9 epitope a) alone or together with a rat IgG2a, anti-CD8 mAb (YTS 105.18 epitope c) for 3 weeks. Animals received three antibody injections per week to a total antibody dose of 7 mg. One week after the last mAb injection, mice were bled and PBL stained with biotinylated anti-Thy-1 (open bars), anti-CD4 epitope b (solid bars), anti-CD8 eptiopes a and b (shaded bars) followed by FITC-streptavidin, or FITC-anti-rat Ig to detect in vivo bound mAb (broken bars).The results were analyzed by FCM with linear amplifiers and mean fluorescence values are indicated.
Qin, S., Wise, M., Cobbold, S. P., Leong, L., M. Kong, Y. C., Parnes, J. R., & Waldmann, H. (1990). Induction of tolerance in peripheral T cells with monoclonal antibodies. European journal of immunology, 20(12), 2737-2745.
Specifications
- Immunogen
- Mouse CD8 antigen, alpha chain; Cd8a
- Host Species
- Rat
- Type
- Rat IgG
- Specificity
- Mouse CD8A
- Species Reactivity
- Mouse
- Clone
- YTS 105.18
- Applications
- Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody YTS 105.18 has been reported in applications of Neutralization, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-369-VHH | Recombinant Anti-Mouse Cd8a VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| NABG-041 | Recombinant Anti-Human CD8A VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-184-S(P) | Recombinant Anti-human CD8A Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| MHH-184-S(P) | Recombinant Human Anti-human CD8A Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| PFBZ-144 | Rat Anti-CD8A Recombinant Antibody (clone YTS 105.18); Fab Fragment | Neut | Rat Fab |
| HPAB-0188-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody; scFv Fragment (HPAB-0188-CN-S(P)) | FC | Mouse scFv |
| HPAB-S0183-YC-S(P) | Rat Anti-Cd8a Recombinant Antibody (clone YTS105.18); scFv Fragment | ELISA | Rat scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-144 | Rat Anti-CD8A Recombinant Antibody (clone YTS 105.18); scFv Fragment | Neut | Rat scFv |
| FAMAB-0009-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone CT8); Fab Fragment | FC | Mouse Fab |
| FAMAB-0010-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone EP72); Fab Fragment | FC | Mouse Fab |
| FAMAB-0011-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone 3-298); Fab Fragment | FC | Mouse Fab |
| FAMAB-0037-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone 6G5); Fab Fragment | WB, IP, IF | Mouse Fab |
Mouse Antibody
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-428CQ | Mouse Anti-CD8A Recombinant Antibody (clone UCHT4) | FC, IHC-Fr, IHC-P, IP, Separation, Block | Mouse IgG2a, κ |
| NEUT-431CQ | Rat Anti-CD8A Recombinant Antibody (clone 53-6.7) | ICC, IF, FC, IHC-Fr, IP, FuncS, Block | Rat IgG2a, κ |
| NEUT-432CQ | Rat Anti-CD8A Recombinant Antibody (clone MAB0811) | Block, Depletion | Rat IgG2a |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-429CQ | Mouse Anti-CD8A Recombinant Antibody (clone CT-8) | Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC | Mouse IgG1, κ |
| NEUT-430CQ | Mouse Anti-CD8A Recombinant Antibody (clone LT8) | WB, Neut | Mouse IgG1 |
| NEUT-433CQ | Mouse Anti-CD8A Recombinant Antibody (clone 76-2-11) | Neut, FC, IHC-Fr, IHC-P, IP | Mouse IgG2a, κ |
| NEUT-434CQ | Mouse Anti-Cd8a Recombinant Antibody (clone OX-8) | WB, Neut, FC, IHC, IHC-Fr, IHC-P, IP, CyTOF® | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0582 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS582AB) | IHC | Rabbit IgG |
| MOR-0589 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS589AB) | IHC-P | Rabbit IgG |
| MOR-0590 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS590AB), PE | FC | Rabbit IgG |
| MOR-0057-FY | Rabbit Anti-CD8A Recombinant Antibody (clone AFY0028) | ICC, IF, IHC-P, WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0317-CN | Rabbit Anti-CD8A Recombinant Antibody (clone CBACN-121) | WB, IF, IHC | Rabbit IgG |
| HPAB-0037-YJ | Human Anti-CD8A Recombinant Antibody (HPAB-0037-YJ) | IA | Humanized IgG |
| MOB-247YJL | Rabbit Anti-CD8A Recombinant Antibody (clone CBC231) | FC, ICC, IF | Rabbit IgG |
| MOB-0248YJL | Rabbit Anti-CD8A Recombinant Antibody (clone CBC232) | ELISA | Rabbit IgG |
| MOB-248YJL | Rabbit Anti-CD8A Recombinant Antibody (clone CBC233) | FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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