Mouse Anti-Gypa Recombinant Antibody (clone NNA7) (CAT#: PABZ-089)
Recombinant Mouse Antibody (NNA7) is capable of binding to Mouse Gypa, expressed in Chinese Hamster Ovary cells (CHO). The NNA7 antibody recognizes the human N-type blood-group antigen comprised of the N-terminal glycopeptide of glycophorin A (GPA).
Specific Inquiry
Figure 1 Detection of glycophorin A on Western blots using N92 mAb, N92 Fab, and NNA7 Fab.
M-type glycophorin A (lanes 1, 3, and 5) and N-type glycophorin A (lanes 2, 4, and 6) were purified from human RBCs, separated by SDS-PAGE, and blotted onto nitrocellulose paper. The blots were then probed with 100 nM N92 mAb (lanes 1 and 2), 200 nM N92 Fab (lanes 3 and 4), and 10 nM NNA7 Fab (lanes 5 and 6). The migration positions of the glycophorin A monomer and dimer and of the glycophorin B monomer and dimer are shown. Glycophorin B, which is also present in small amounts in these preparations, encodes the N blood group Ag even when it is isolated from individuals who are homozygous for the M blood group Ag; this explains the reactivity in lanes 1 and 5.
Czerwinski, M., Siemaszko, D., Siegel, D. L., & Spitalnik, S. L. (1998). Only selected light chains combine with a given heavy chain to confer specificity for a model glycopeptide antigen. The Journal of Immunology, 160(9), 4406-4417.
Figure 2 Comparison of binding to glycophorin A of the parental N92 mAb, parental N92-derived soluble Fab, and soluble Fab derived from the N92 Fd:light chain libraries.
MAbs and soluble Fab were purified, and microtiter plates were coated with N-type glycophorin A as described in Materials and Methods. Defined concentrations of the N92 parental mAb (closed circles), the N92 parental-derived soluble Fab (open squares), and the G11 (open circles), NNA7 (triangles) and C1 (closed squares) soluble Fab isolated from the N92 Fd:light chain libraries were added to corresponding wells. The bound Ig proteins were then detected as described in Figure 5. In A, all the soluble Fab are compared with each other; in B, the library-derived Fab are compared with the parental, hybridoma-derived mAb. The experiments in A and B were performed on separate occasions.
Czerwinski, M., Siemaszko, D., Siegel, D. L., & Spitalnik, S. L. (1998). Only selected light chains combine with a given heavy chain to confer specificity for a model glycopeptide antigen. The Journal of Immunology, 160(9), 4406-4417.
Specifications
- Immunogen
- Mouse glycophorin A
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Mouse Gypa
- Species Reactivity
- Mouse
- Clone
- NNA7
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody NNA7 has been reported in applications of WB, ELISA. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- GPA; CD235a; AI853584
- Gene ID
- 14934
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1586z | Mouse Anti-GYPA Recombinant Antibody (clone 26C8) | WB, FC, IF, IHC | Mouse IgG1, κ |
| MOB-0295MZ | Recombinant Mouse Anti-Human Glycophorin A Antibody (clone KC160) | IHC | Mouse antibody |
| MOB-078CQ | Rat Anti-GYPA Recombinant Antibody (clone YTH89.1) | FC, IHC-P, IHC-Fr | Rat IgG2b |
| MOB-137CQ | Mouse Anti-GYPA Recombinant Antibody (clone YC100) | WB, IF, IP, IHC-P | Mouse IgG1 |
| FAMAB-1150CQ | Human Anti-GYPA Recombinant Antibody (clone JC159) | WB, IHC | Human IgG1 |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-073 | Recombinant Anti-Human GYPA VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-151CT | Human Anti-GYPA Recombinant Antibody (TAB-151CT) | ELISA, FC, WB | VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-089 | Mouse Anti-Gypa Recombinant Antibody (clone NNA7); scFv Fragment | WB, ELISA | Mouse scFv |
| FAMAB-0159CQ-S(P) | Mouse Anti-GYPA Recombinant Antibody (clone 6A7); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0160CQ-S(P) | Mouse Anti-GYPA Recombinant Antibody (clone A09); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0161CQ-S(P) | Mouse Anti-GYPA Recombinant Antibody (clone M2A1); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-1164-FY-F(E) | Human Anti-GYPA Recombinant Antibody; Fab Fragment (HPAB-1164-FY-F(E)) | Activ | Humanized Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-150CT | Mouse Anti-GYPA Recombinant Antibody (TAB-150CT) | Funcs, PD, PK, ELISA | Mouse IgG1 |
| TAB-150CT-S(P) | Mouse Anti-GYPA Recombinant Antibody; scFv Fragment (TAB-150CT-S(P)) | ELISA | Mouse scFv |
| TAB-150CT-F(E) | Mouse Anti-GYPA Recombinant Antibody; Fab Fragment (TAB-150CT-F(E)) | ELISA | Mouse Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1548 | Rabbit Anti-GYPA Recombinant Antibody (clone DS1548AB) | WB, IHC | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0159CQ-F(E) | Mouse Anti-GYPA Recombinant Antibody (clone 6A7); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0160CQ-F(E) | Mouse Anti-GYPA Recombinant Antibody (clone A09); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0161CQ-F(E) | Mouse Anti-GYPA Recombinant Antibody (clone M2A1); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0162CQ-F(E) | Mouse Anti-GYPA Recombinant Antibody (clone AH7); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-1164-FY-S(P) | Human Anti-GYPA Recombinant Antibody; scFv Fragment (HPAB-1164-FY-S(P)) | Activ | Humanized scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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